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Beta-defensins-2 expressions in gingival epithelium cells after probiotic Lactobacillus reuteri induction

Dental Journal (Majalah Kedokteran Gigi) · Vol. 49 No. 1 (2016) · hlm. 49–53

DOI
10.20473/j.djmkg.v49.i1.p49-53
ISSN
2442-9740, 1978-3728
Terbit
2016-03-31
Penerbit
Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en
Bahasa
eng

Abstrak

Background: Beta-defensins (BD) are antimicrobial peptides that play a role in defense against pathogens. Beta-defensins (BD) are expressed by a variety of epithelial cells, including gingival epithelium, salivary glands, saliva and salivary duct. BD-1 is expressed constitutively, while BD-2 and BD-3 expressions can be induced by commensal bacteria. Probiotics are commensal bacteria, thus L. reuteri as probiotic bacteria may act as "inducer” for BD-2 in epithelial gingiva. S. mutans is the main bacteria causing dental caries and sensitive to BD-2. Purpose: This study was aimed to prove that the administration of probiotic L. reuteri may improve BD-2 expressions in the gingiva epithelium. Method: This study was conducted in vivo using twenty-four male Rattus norvegicus Wistar strains aged 10-12 weeks and weighed 120-150 g. Those rats were randomly divided into four groups, namely negative control group (not induced with L. reuteri or S. mutans), positive control group (induced with S. mutans for 14 days), treatment group 1 (induced with L. reuteri for 14 days and S. mutans for 7 days), and treatment group 2 (induced with L. reuteri and S. mutans for 14 days concurrently). The concentration of L. reuteri used was 4x108cfu/ml, while the concentration of S. mutans was 1x 1010cfu/ml. 0.1 ml of each was dropped in the region of the mandibular incisors. BD-2 expression was calculated using immunohistochemical method. The difference of BD-2 expressions in gingival epithelial cells in the respective groups was analyzed by Anova/SPSS. Results: There were significant differences in BD-2 expressions in gingival epithelial cells in each group based on the results of Anova test (p=0.001). Conclusion: The administration of probiotic L. reuteri is able to increase BD-2 expressions in gingival epithelial cells.

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Rekan penulisOrganisasiArtikel bersama
Ayu Rafania AtikahUniversitas Airlangga1
Hendrik Setia BudiUniversitas Airlangga1
Muhammad LuthfiUniversitas Airlangga1
Rini Devijanti RidwanAfiliasi tidak diketahui1
Sidarningsih SidarningsihUniversitas Airlangga1
Oktaviani Suci LestariUniversitas Airlangga1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/MKG/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Beta-defensins-2 expressions in gingival epithelium cells after probiotic Lactobacillus reuteri induction</dc:title>
	<dc:creator>Kusumaningsih, Tuti</dc:creator>
	<dc:subject xml:lang="en-US">beta defensins-2 expression</dc:subject>
	<dc:subject xml:lang="en-US">gingival epithelium</dc:subject>
	<dc:subject xml:lang="en-US">probiotic</dc:subject>
	<dc:subject xml:lang="en-US">L. reuteri</dc:subject>
	<dc:subject xml:lang="en-US">S. mutans</dc:subject>
	<dc:description xml:lang="en-US">Background: Beta-defensins (BD) are antimicrobial peptides that play a role in defense against pathogens. Beta-defensins (BD) are expressed by a variety of epithelial cells, including gingival epithelium, salivary glands, saliva and salivary duct. BD-1 is expressed constitutively, while BD-2 and BD-3 expressions can be induced by commensal bacteria. Probiotics are commensal bacteria, thus L. reuteri as probiotic bacteria may act as "inducer” for BD-2 in epithelial gingiva. S. mutans is the main bacteria causing dental caries and sensitive to BD-2. Purpose: This study was aimed to prove that the administration of probiotic L. reuteri may improve BD-2 expressions in the gingiva epithelium. Method: This study was conducted in vivo using twenty-four male Rattus norvegicus Wistar strains aged 10-12 weeks and weighed 120-150 g. Those rats were randomly divided into four groups, namely negative control group (not induced with L. reuteri or S. mutans), positive control group (induced with S. mutans for 14 days), treatment group 1 (induced with L. reuteri for 14 days and S. mutans for 7 days), and treatment group 2 (induced with L. reuteri and S. mutans for 14 days concurrently). The concentration of L. reuteri used was 4x108cfu/ml, while the concentration of S. mutans was 1x 1010cfu/ml. 0.1 ml of each was dropped in the region of the mandibular incisors. BD-2 expression was calculated using immunohistochemical method. The difference of BD-2 expressions in gingival epithelial cells in the respective groups was analyzed by Anova/SPSS. Results: There were significant differences in BD-2 expressions in gingival epithelial cells in each group based on the results of Anova test (p=0.001). Conclusion: The administration of probiotic L. reuteri is able to increase BD-2 expressions in gingival epithelial cells.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en</dc:publisher>
	<dc:date>2016-03-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/MKG/article/view/2014</dc:identifier>
	<dc:identifier>10.20473/j.djmkg.v49.i1.p49-53</dc:identifier>
	<dc:source xml:lang="en-US">Dental Journal (Majalah Kedokteran Gigi); Vol. 49 No. 1 (2016): March 2016; 49-53</dc:source>
	<dc:source>2442-9740</dc:source>
	<dc:source>1978-3728</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/MKG/article/view/2014/2191</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 Dental Journal (Majalah Kedokteran Gigi)</dc:rights>
</oai_dc:dc>