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Analysis of anti-Streptococcus sanguinis IgY ability to inhibit Streptococcus sanguinis adherence

Dental Journal (Majalah Kedokteran Gigi) · Vol. 51 No. 1 (2018) · hlm. 33–36

DOI
10.20473/j.djmkg.v51.i1.p33-36
ISSN
2442-9740, 1978-3728
Terbit
2018-03-31
Penerbit
Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en
Bahasa
eng

Abstrak

Background: Streptococcus sanguinis (S. sanguinis), an oral commensal bacterium, is often implicated in infective endocarditis. Its adherence to the tooth surface is the initial step in dental plaque formation. In addition to the important role of S. sanguinis in systemic disease and antimicrobial resistance, it is necessary to develop methods to control dental plaque formation. Immunoglobulin Y (IgY) has been used to prevent bacterial infection. Purpose: The purpose of this study is to analyze the ability of anti-S. sanguinis IgY antibodies to inhibit S. sanguinis adherence to hydroxyapatite (HA) discs as a model of the tooth surface. Methods: Antibodies were produced by immunizing hens with S. sanguinis suspension. Boosters were given three times following the first injection. An agar gel precipitation test (AGPT) was used to detect the presence of anti-S. sanguinis IgY. A bacterial adherence assay was performed twice to analyze the ability of IgY and the optimal concentration required to inhibit bacterial adherence. Results: The formation of a precipitation line using AGPT confirmed the presence of the antibody. In addition, it was shown that the anti-S. sanguinis IgY antibody could inhibit bacterial adherence to HA. Statistical analysis using One-way ANOVA revealed a significant difference in the optical density (OD) value between the groups (p<0.05). The results of electron microscopy scanning confirmed the quantitative analysis by means of a bacterial adherence test. Conclusion: Anti-S. sanguinis IgY has the ability to inhibit adherence of S. sanguinis to HA discs at an optimal concentration of 30%. The inhibitive effect was stronger in the presence of saliva.

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  • Universitas Gadjah Mada
  • Duta Wacana Christian University
  • Sebelas Maret University
  • IPB University
  • Universitas Airlangga
  • Sriwijaya University
  • Taipei Medical University
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Rekan penulisOrganisasiArtikel bersama
Michael Haryadi WibowoUniversitas Gadjah Mada4
Wayan Tunas ArtamaUniversitas Gadjah Mada4
Marsetyawan H. N. E. SoesatyoUniversitas Gadjah Mada3
Denise Utami PutriTaipei Medical University3
Arum SetiawanSriwijaya University2
Mammed SagiUniversitas Gadjah Mada2
Istriyati IstriyatiUniversitas Gadjah Mada2
Sofia Mubarika HaryanaUniversitas Gadjah Mada2
Christiane Marlene SooaiDuta Wacana Christian University2
Ceny Gloria LaropeDuta Wacana Christian University2
Haryo Dimasto KristiyantoDuta Wacana Christian University2
Triyanto TriyantoUniversitas Gadjah Mada2
Nursyirwani NursyirwaniRiau University2
A. E. T. H. WahyuniUniversitas Gadjah Mada2
Jaka WidadaUniversitas Gadjah Mada2
Alimuddin AlimuddinIPB University2
Sebastian MarginoUniversitas Gadjah Mada2
Widodo WidodoIPB University2
Sri DarmawatiUniversitas Muhammadiyah Semarang2
Langkah SembiringUniversitas Gadjah Mada2
Tri UntariAfiliasi tidak diketahui2
Siti SunarintyasUniversitas Gadjah Mada2
Pinandi Sri PudyaniUniversitas Gadjah Mada2
Archadian NuryantiUniversitas Gadjah Mada1
Dian Yosi ArinawatiUniversitas Gadjah Mada1
Supriatno SupriatnoUniversitas Gadjah Mada1
Ika Dewi AnaUniversitas Gadjah Mada1
Tita Ratya UtariMuhammadiyah University of Yogyakarta1
Juni HandajaniUniversitas Gadjah Mada1
Yulita KristantiUniversitas Gadjah Mada1
Risya CilmiatySebelas Maret University1
Mandojo RukmoUniversitas Airlangga1
Afiono Agung PrasetyoSebelas Maret University1
Khilyat Ulin Nur ZainiSebelas Maret University1
Suhartono Taat PutraUniversitas Airlangga1
Banun KusumawardaniUniversitas Jember1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/MKG/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Analysis of anti-Streptococcus sanguinis IgY ability to inhibit Streptococcus sanguinis adherence</dc:title>
	<dc:creator>Hutomo, Suryani</dc:creator>
	<dc:creator>Susilowati, Heni</dc:creator>
	<dc:creator>Agustina, Dewi</dc:creator>
	<dc:creator>Asmara, Widya</dc:creator>
	<dc:subject xml:lang="en-US">Streptococcus sanguinis</dc:subject>
	<dc:subject xml:lang="en-US">IgY</dc:subject>
	<dc:subject xml:lang="en-US">bacterial adherence</dc:subject>
	<dc:description xml:lang="en-US">Background: Streptococcus sanguinis (S. sanguinis), an oral commensal bacterium, is often implicated in infective endocarditis. Its adherence to the tooth surface is the initial step in dental plaque formation. In addition to the important role of S. sanguinis in systemic disease and antimicrobial resistance, it is necessary to develop methods to control dental plaque formation. Immunoglobulin Y (IgY) has been used to prevent bacterial infection. Purpose: The purpose of this study is to analyze the ability of anti-S. sanguinis IgY antibodies to inhibit S. sanguinis adherence to hydroxyapatite (HA) discs as a model of the tooth surface. Methods: Antibodies were produced by immunizing hens with S. sanguinis suspension. Boosters were given three times following the first injection. An agar gel precipitation test (AGPT) was used to detect the presence of anti-S. sanguinis IgY. A bacterial adherence assay was performed twice to analyze the ability of IgY and the optimal concentration required to inhibit bacterial adherence. Results: The formation of a precipitation line using AGPT confirmed the presence of the antibody. In addition, it was shown that the anti-S. sanguinis IgY antibody could inhibit bacterial adherence to HA. Statistical analysis using One-way ANOVA revealed a significant difference in the optical density (OD) value between the groups (p&amp;lt;0.05). The results of electron microscopy scanning confirmed the quantitative analysis by means of a bacterial adherence test. Conclusion: Anti-S. sanguinis IgY has the ability to inhibit adherence of S. sanguinis to HA discs at an optimal concentration of 30%. The inhibitive effect was stronger in the presence of saliva.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en</dc:publisher>
	<dc:date>2018-03-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/MKG/article/view/7557</dc:identifier>
	<dc:identifier>10.20473/j.djmkg.v51.i1.p33-36</dc:identifier>
	<dc:source xml:lang="en-US">Dental Journal (Majalah Kedokteran Gigi); Vol. 51 No. 1 (2018): March 2018; 33-36</dc:source>
	<dc:source>2442-9740</dc:source>
	<dc:source>1978-3728</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/MKG/article/view/7557/4916</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 Dental Journal (Majalah Kedokteran Gigi)</dc:rights>
</oai_dc:dc>