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The activity of polyclonal IgY derived from Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in inhibiting colonization of Fusobacterium nucleatum and Streptococcus sanguinis

Dental Journal (Majalah Kedokteran Gigi) · Vol. 52 No. 2 (2019) · hlm. 81–85

DOI
10.20473/j.djmkg.v52.i2.p81-85
ISSN
2442-9740, 1978-3728
Terbit
2019-06-30
Penerbit
Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en
Bahasa
eng

Abstrak

Background: Fusobacterium nucleatum (F. nucleatum) and Streptococcus sanguinis (S. sanguinis) play a role in dental plaque formation which leads to periodontitis. Immunoglobulin Y (IgY) is present in both serum and egg yolk and can bind to the surface components of bacteria. F. nucleatum and S. sanguinis feature the same type of IV pili as Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Saliva binding protein (SsaB) in S. sanguinis is a FimA homolog. FimA constitutes a surface element of Porphyromonas gingivalis (P. gingivalis). F. nucleatum and P. gingivalis possess the same outer membrane protein (OMP) molecular mass. Purpose: The study aimed to determine the activity of A. actinomycetemcomitans and P. gingivalis polyclonal IgY present in serum and egg yolk that can inhibit colonization of F. nucleatum and S. sanguinis. Methods: IgY samples were diluted with phosphate buffer saline (PBS). Several holes were made in the nutrient medium with 10 μl antigen (F. nucleatum/S. sanguinis) being inserted into the center hole. 10 μl PBS, 1:1, 1:2, 1:4, 1:8, 1:16 A. actinomycetemcomitans or P. gingivalis polyclonal IgY were subsequently introduced into the surrounding holes. The results of incubation at 37°C were observed after 24-48 hours. Kruskal Wallis and MannWhitney tests were administered to analyse the data. Results: A. actinomycetemcomitans and P. gingivalis polyclonal IgY groups in serum showed a precipitation line at dilution ratios of 1:1 and 1:2, whereas in egg yolk this occurred only at a 1:1 dilution ratio with F. nucleatum and S. sanguinis bacteria in this study. No significant differences were evident between each dilution (p>0.05) and none existed between serum and egg yolk (p>0.05). Conclusion: IgY polyclonal of A. actinomycetemcomitans and P. gingivalis in both serum and egg yolk initiate activities that can inhibit colonization of F. nucleatum and S. sanguinis.

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Rekan penulisOrganisasiArtikel bersama
Wisnu Setyari JuliastutiAfiliasi tidak diketahui2
Indeswati DiyatriAfiliasi tidak diketahui2
Hendrik Setia BudiAfiliasi tidak diketahui2
Mohammed AljunaidAfiliasi tidak diketahui2
Ayu Rafania AtikahAfiliasi tidak diketahui1
Muhammad LuthfiAfiliasi tidak diketahui1
Eha Renwi AstutiUniversitas Airlangga1
Ramadhan Hardani PutraUniversitas Airlangga1
Willy WijayaAfiliasi tidak diketahui1
Diah Savitri ErnawatiAfiliasi tidak diketahui1
Ika Rhisty Cendana SariAfiliasi tidak diketahui1
R. Darmawan SetijantoAfiliasi tidak diketahui1
Nova Andriani HepitariaAfiliasi tidak diketahui1
Markus Budi RahardjoAfiliasi tidak diketahui1
Ira WidjiastutiAfiliasi tidak diketahui1
Nur DianawatiAfiliasi tidak diketahui1
Wahyu SetyariniAfiliasi tidak diketahui1
K. KuntamanAfiliasi tidak diketahui1
Ira ArundinaAfiliasi tidak diketahui1
Anis IrmawatiAfiliasi tidak diketahui1
Lia Aulia RachmaAfiliasi tidak diketahui1
Muhammad Naufal HattaAfiliasi tidak diketahui1
Yeka RamadhaniAfiliasi tidak diketahui1
Riski Rahayu Putri RahmasariAfiliasi tidak diketahui1
Kinanti Nasywa PrajnasariAfiliasi tidak diketahui1
Moh. Malik AlhakimAfiliasi tidak diketahui1
Hesham Mohammed Al-SharaniAfiliasi tidak diketahui1
T. TantianaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/MKG/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">The activity of polyclonal IgY derived from Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in inhibiting colonization of Fusobacterium nucleatum and Streptococcus sanguinis</dc:title>
	<dc:creator>Lestari, Oktaviani Suci</dc:creator>
	<dc:creator>Ridwan, Rini Devijanti</dc:creator>
	<dc:creator>Kusumaningsih, Tuti</dc:creator>
	<dc:creator>Sidarningsih, S.</dc:creator>
	<dc:subject xml:lang="en-US">Aggregatibacter actinomycetemcomitans</dc:subject>
	<dc:subject xml:lang="en-US">Fusobacterium nucleatum</dc:subject>
	<dc:subject xml:lang="en-US">IgY</dc:subject>
	<dc:subject xml:lang="en-US">Porphyromonas gingivalis</dc:subject>
	<dc:subject xml:lang="en-US">Streptococcus sanguinis</dc:subject>
	<dc:description xml:lang="en-US">Background: Fusobacterium nucleatum (F. nucleatum) and Streptococcus sanguinis (S. sanguinis) play a role in dental plaque formation which leads to periodontitis. Immunoglobulin Y (IgY) is present in both serum and egg yolk and can bind to the surface components of bacteria. F. nucleatum and S. sanguinis feature the same type of IV pili as Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Saliva binding protein (SsaB) in S. sanguinis is a FimA homolog. FimA constitutes a surface element of Porphyromonas gingivalis (P. gingivalis). F. nucleatum and P. gingivalis possess the same outer membrane protein (OMP) molecular mass. Purpose: The study aimed to determine the activity of A. actinomycetemcomitans and P. gingivalis polyclonal IgY present in serum and egg yolk that can inhibit colonization of F. nucleatum and S. sanguinis. Methods: IgY samples were diluted with phosphate buffer saline (PBS). Several holes were made in the nutrient medium with 10 μl antigen (F. nucleatum/S. sanguinis) being inserted into the center hole. 10 μl PBS, 1:1, 1:2, 1:4, 1:8, 1:16 A. actinomycetemcomitans or P. gingivalis polyclonal IgY were subsequently introduced into the surrounding holes. The results of incubation at 37°C were observed after 24-48 hours. Kruskal Wallis and MannWhitney tests were administered to analyse the data. Results: A. actinomycetemcomitans and P. gingivalis polyclonal IgY groups in serum showed a precipitation line at dilution ratios of 1:1 and 1:2, whereas in egg yolk this occurred only at a 1:1 dilution ratio with F. nucleatum and S. sanguinis bacteria in this study. No significant differences were evident between each dilution (p&amp;gt;0.05) and none existed between serum and egg yolk (p&amp;gt;0.05). Conclusion: IgY polyclonal of A. actinomycetemcomitans and P. gingivalis in both serum and egg yolk initiate activities that can inhibit colonization of F. nucleatum and S. sanguinis.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en</dc:publisher>
	<dc:date>2019-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/MKG/article/view/12646</dc:identifier>
	<dc:identifier>10.20473/j.djmkg.v52.i2.p81-85</dc:identifier>
	<dc:source xml:lang="en-US">Dental Journal (Majalah Kedokteran Gigi); Vol. 52 No. 2 (2019): June 2019; 81-85</dc:source>
	<dc:source>2442-9740</dc:source>
	<dc:source>1978-3728</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/MKG/article/view/12646/8415</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 Dental Journal (Majalah Kedokteran Gigi)</dc:rights>
</oai_dc:dc>