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Sperm Preservation using Freeze-Drying Method

HAYATI Journal of Biosciences · Vol. 12 No. 1 (2009)

DOI
10.4308/hjb.12.1.41
ISSN
2086-4094, 1978-3019
Terbit
2009-11-30
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse.

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  • Universitas Halu Oleo
  • University of Brawijaya
  • IPB University
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
La Ode NafiuUniversitas Halu Oleo5
Muhammad Amrullah PagalaUniversitas Halu Oleo4
Muh. RusdinUniversitas Halu Oleo2
Rusli BadaruddinUniversitas Halu Oleo2
Achmad Selamet AkuUniversitas Halu Oleo2
Deki ZulkarnainUniversitas Halu Oleo2
Aryani Sismin SatyaningtijasAfiliasi tidak diketahui2
Luki AbdullahIPB University1
Asnath Maria FuahAfiliasi tidak diketahui1
Sri RahayuUniversity of Brawijaya1
Rachmat SomanjayaAfiliasi tidak diketahui1
Rahim AkaUniversitas Halu Oleo1
Viny Aprilia KesumanengsihAfiliasi tidak diketahui1
Nursanty AsminayaAfiliasi tidak diketahui1
Dian AgustinaAfiliasi tidak diketahui1
La Ode Abdul Karim IshakAfiliasi tidak diketahui1
Meri AstutiAfiliasi tidak diketahui1
Andi Murlina TasseUniversitas Halu Oleo1
Purnaning Dhian IsnaeniAfiliasi tidak diketahui1
Wa Ode Al JumiatiAfiliasi tidak diketahui1
La Ode Muh. MunadiAfiliasi tidak diketahui1
Fahry AlbarAfiliasi tidak diketahui1
Malida MalidaAfiliasi tidak diketahui1
Musram AbadiUniversitas Halu Oleo1
Dedi DediAfiliasi tidak diketahui1
Fitrianingsih FitrianingsihAfiliasi tidak diketahui1
Nur Santy AsminayaUniversitas Halu Oleo1
Ummul KhairiyahAfiliasi tidak diketahui1
Gusti Ayu Kade SutariatiUniversitas Halu Oleo1
Idrus SalamUniversitas Halu Oleo1
Syamsuddin SyamsuddinAfiliasi tidak diketahui1
Huda Salahudin DarusmanAfiliasi tidak diketahui1
Andhika Yudha PrawiraAfiliasi tidak diketahui1
Wartika Rosa FaridaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Sperm Preservation using Freeze-Drying Method</dc:title>
	<dc:creator xml:lang="en">SAILI, TAKDIR</dc:creator>
	<dc:creator xml:lang="en">PANGESTU, MULYOTO</dc:creator>
	<dc:creator xml:lang="en">SETIADI, MOHAMAD AGUS</dc:creator>
	<dc:creator xml:lang="en">AGUNGPRIYONO, SRIHADI</dc:creator>
	<dc:creator xml:lang="en">R. TOELIHERE, MOZES</dc:creator>
	<dc:creator xml:lang="en">BOEDIONO, ARIEF</dc:creator>
	<dc:description xml:lang="en">Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2009-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/170</dc:identifier>
	<dc:identifier>10.4308/hjb.12.1.41</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 12 No. 1 (2005): March 2005; 41</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/170/37</dc:relation>
</oai_dc:dc>