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Maturation Rate of Ovine Oocytes from Different Reproductive Status and Maturation Medium

HAYATI Journal of Biosciences · Vol. 13 No. 4 (2009)

DOI
10.4308/hjb.13.4.131
ISSN
2086-4094, 1978-3019
Terbit
2009-12-02
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

The aim of the present study was to determine the number of follicles, oocyte quality and maturation rate of oocytes from pairs of ovary with different reproductive status in two maturation medium, TCM-199 as control and CR1aa as treatment. The pairs of ovary were classified into four groups: (i) ovaries with corpus luteum (CL) and dominant follicle (DF), (ii) ovaries with CL, without DF, (iii) ovaries with DF, without CL, (iv) ovaries without both CL and DF. Results of the experiment revealed that the greatest number of follicles was observed from ovary with CL without DF (15.88 + 10.68), although not significantly different (P > 0.05) with other status of ovaries. The lowest number (P < 0.05) of A grade oocytes was found from ovary with DF without CL (1.20 + 1.10). The percentage of Metaphase II was highest in TCM-199 (75.51%) with oocytes from ovaries with CL and DF, and the lowest with oocytes from ovaries with DF without CL in TCM-199 and CR1aa (42.86 and 30.95%). The study suggested that the number of oocytes with A grade were influenced by the reproductive status of ovaries. The maturation rate of A grade oocytes was influenced by the quality of oocytes and the composition of maturation medium. Key words: reproductive status, corpus luteum, dominant follicles, TCM-199, CR1aa

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Srihadi AgungpriyonoAfiliasi tidak diketahui5
HERDISAfiliasi tidak diketahui4
Takdir SailiAfiliasi tidak diketahui4
Muhammad RizalUniversitas Halu Oleo3
Mokhamad FahrudinAfiliasi tidak diketahui3
Berry JuliandiAfiliasi tidak diketahui3
Iman SupriatnaAfiliasi tidak diketahui3
Luki AbdullahIPB University2
Asnath Maria FuahAfiliasi tidak diketahui2
Sri RahayuHasanuddin University2
Rachmat SomanjayaAfiliasi tidak diketahui2
Kelvin YapriantoAfiliasi tidak diketahui2
Indra BachtiarAfiliasi tidak diketahui2
Adi WinartoAfiliasi tidak diketahui2
ITA DJUWITAAfiliasi tidak diketahui2
. YulnawatiAfiliasi tidak diketahui2
Vista BudiariatiAfiliasi tidak diketahui2
Dwi BudionoAfiliasi tidak diketahui2
Ni Wayan Kurniani KarjaIPB University2
Harry MurtiAfiliasi tidak diketahui2
MULYOTO PANGESTUAfiliasi tidak diketahui2
Aryani Sismin SatyaningtijasAfiliasi tidak diketahui1
Resti Rahma DiantiAfiliasi tidak diketahui1
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Wining AstiniAfiliasi tidak diketahui1
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Yushinta FujayaAfiliasi tidak diketahui1
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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Maturation Rate of Ovine Oocytes from Different Reproductive Status and Maturation Medium</dc:title>
	<dc:creator xml:lang="en">BOEDIONO, ARIEF</dc:creator>
	<dc:creator xml:lang="en">YULNAWATI, YULNAWATI</dc:creator>
	<dc:creator xml:lang="en">SETIADI, MOHAMAD AGUS</dc:creator>
	<dc:description xml:lang="en">The aim of the present study was to determine the number of follicles, oocyte quality and maturation rate of oocytes from pairs of ovary with different reproductive status in two maturation medium, TCM-199 as control and CR1aa as treatment. The pairs of ovary were classified into four groups: (i) ovaries with corpus luteum (CL) and dominant follicle (DF), (ii) ovaries with CL, without DF, (iii) ovaries with DF, without CL, (iv) ovaries without both CL and DF. Results of the experiment revealed that the greatest number of follicles was observed from ovary with CL without DF (15.88 + 10.68), although not significantly different (P &gt; 0.05) with other status of ovaries. The lowest number (P &lt; 0.05) of A grade oocytes was found from ovary with DF without CL (1.20 + 1.10). The percentage of Metaphase II was highest in TCM-199 (75.51%) with oocytes from ovaries with CL and DF, and the lowest with oocytes from ovaries with DF without CL in TCM-199 and CR1aa (42.86 and 30.95%). The study suggested that the number of oocytes with A grade were influenced by the reproductive status of ovaries. The maturation rate of A grade oocytes was influenced by the quality of oocytes and the composition of maturation medium. Key words: reproductive status, corpus luteum, dominant follicles, TCM-199, CR1aa </dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2009-12-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/220</dc:identifier>
	<dc:identifier>10.4308/hjb.13.4.131</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 13 No. 4 (2006): December 2006; 131</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/220/86</dc:relation>
</oai_dc:dc>