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Extracellular Protease Activity of Enteropathogenic Escherechia coli on Mucin Substrate

HAYATI Journal of Biosciences · Vol. 14 No. 1 (2009)

DOI
10.4308/hjb.14.1.36
ISSN
2086-4094, 1978-3019
Terbit
2009-12-02
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Enteropathogenic Escherichia coli (EPEC) causes gastrointestinal infections in human. EPEC invasion was initiated by attachment and aggressive colonization on intestinal surface. Attachment of EPEC alter the intestine mucosal cells. Despite this, the pathogenic mechanism of EPEC infectior has not been fully understood. This research hypothesizes that extracellular proteolytic enzymes is necessary for EPEC colonization. The enzyme is secreted into gastrointestinal milieu and presumably destroy mucus layer cover the gastrointestinal tract. The objective of this study was to assay EPEC extracellular protease enzyme by using mucin substrate. The activity of EPEC extracellular proteolytic enzyme on 1% mucin substrate was investigated. Non-pathogenic E. coli was used as a negative control. Positive and tentative controls were Yersinia enterocolitica and Salmonella. Ten EPEC strains were assayed, seven of them were able to degrade mucin, and the highest activity was produced by K1.1 strain. Both positive and tentative controls also showed the ability to digest 0.20% mucin. Key words: EPEC, protease, mucin, diarrhea

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  • IPB University
  • Universitas Papua
  • Sunan Gunung Djati State Islamic University Bandung
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Rekan penulisOrganisasiArtikel bersama
Aris Tri WahyudiIPB University6
Iman RusmanaAfiliasi tidak diketahui5
Antonius SuwantoAfiliasi tidak diketahui4
Rika Indri AstutiAfiliasi tidak diketahui4
Sipriyadi SipriyadiAfiliasi tidak diketahui2
Risky Hadi WibowoAfiliasi tidak diketahui2
Maggy Thenawidjaya SuhartonoAfiliasi tidak diketahui2
BAMBANG SURYOBROTOAfiliasi tidak diketahui2
Alex HartanaAfiliasi tidak diketahui2
Yulin LestariAfiliasi tidak diketahui2
Iah Novi MaslahahAfiliasi tidak diketahui1
Tri Asmira DamayantiAfiliasi tidak diketahui1
HENDRA PARDEDEAfiliasi tidak diketahui1
Triadiati TriadiatiAfiliasi tidak diketahui1
ANGELIA REZTY FITRIANI SITUMORANGAfiliasi tidak diketahui1
BRAMANTYO JATI PRASOJOAfiliasi tidak diketahui1
. QATRUNNADAAfiliasi tidak diketahui1
TETRI WIDIYANIAfiliasi tidak diketahui1
Elda Irma Jeanne Joice KawulurUniversitas Papua1
Dewi SartikaSunan Gunung Djati State Islamic University Bandung1
MIRNAWATI SUDARWANTOAfiliasi tidak diketahui1
Anik KusmiatunAfiliasi tidak diketahui1
Min RahminiwatiAfiliasi tidak diketahui1
Budi WirawanAfiliasi tidak diketahui1
Zulhaerati ZulhaeratiAfiliasi tidak diketahui1
Nabila Tsoerayya Gustia PudjasAfiliasi tidak diketahui1
Lisdar I SudirmanAfiliasi tidak diketahui1
ESTI PUSPITASARIAfiliasi tidak diketahui1
Muhamad Azwar SyahAfiliasi tidak diketahui1
Andreas Adhi SatyaAfiliasi tidak diketahui1
Ririn Herni PutriAfiliasi tidak diketahui1
Alina AkhdiyaAfiliasi tidak diketahui1
Riziq Ilham NurfahmiAfiliasi tidak diketahui1
Ika Roostika TambunanAfiliasi tidak diketahui1
Laksmi AmbarsariAfiliasi tidak diketahui1
Rury Eryna PutriAfiliasi tidak diketahui1
Akhmad Endang Zainal HasanAfiliasi tidak diketahui1
Siti SholekhaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Extracellular Protease Activity of Enteropathogenic Escherechia coli on Mucin Substrate</dc:title>
	<dc:creator xml:lang="en">BUDIARTI, SRI</dc:creator>
	<dc:creator xml:lang="en">MUBARIK, NISA RACHMANIA</dc:creator>
	<dc:description xml:lang="en">Enteropathogenic Escherichia coli (EPEC) causes gastrointestinal infections in human. EPEC invasion was initiated by attachment and aggressive colonization on intestinal surface. Attachment of EPEC alter the intestine mucosal cells. Despite this, the pathogenic mechanism of EPEC infectior has not been fully understood. This research hypothesizes that extracellular proteolytic enzymes is necessary for EPEC colonization. The enzyme is secreted into gastrointestinal milieu and presumably destroy mucus layer cover the gastrointestinal tract. The objective of this study was to assay EPEC extracellular protease enzyme by using mucin substrate. The activity of EPEC extracellular proteolytic enzyme on 1% mucin substrate was investigated. Non-pathogenic E. coli was used as a negative control. Positive and tentative controls were Yersinia enterocolitica and Salmonella. Ten EPEC strains were assayed, seven of them were able to degrade mucin, and the highest activity was produced by K1.1 strain. Both positive and tentative controls also showed the ability to digest 0.20% mucin. Key words: EPEC, protease, mucin, diarrhea </dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2009-12-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/235</dc:identifier>
	<dc:identifier>10.4308/hjb.14.1.36</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 14 No. 1 (2007): March 2007; 36</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/235/101</dc:relation>
</oai_dc:dc>