Melola Discovery

← Melola Discovery

Jurnal SINTA 1

Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring

HAYATI Journal of Biosciences · Vol. 14 No. 2 (2009)

DOI
10.4308/hjb.14.2.54
ISSN
2086-4094, 1978-3019
Terbit
2009-12-02
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

A functional gene containing an Open Reading Frame (ORF) encoding a?-1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for 213 amino acids. The amino acid sequence analysis using BLAST showed that the gene has high homology (93%) with xylanase gene from Bacillus subtilis. The gene showed its function when it was subcloned into an expression vector and overexpressed in E. coli. The crude extract of the recombinant enzyme had activity for 170 U/ml at 50 oC. The result of this work showed that metagenomic approach was a powerful short cut method to obtain recombinant biocatalyst that was useful for industrial application. Key words:?-1, 4-endoxylanase, metagenomic DNA, Pawan-Riau hot-spring

Jaringan kolaborasi para penulis

40 penulis · 149 tautan kepenulisan bersama · 57 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • National Research and Innovation Agency
  • University of Brawijaya
  • Badan Pengkajian dan Penerapan Teknologi
  • University of Indonesia
  • National Center for Genetic Engineering and Biotechnology
  • IPB University
  • Universitas Jenderal Achmad Yani
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Sri Rezeki WulandariNational Research and Innovation Agency4
Niknik NurhayatiNational Research and Innovation Agency3
Gabriela Christy SabbathiniNational Research and Innovation Agency3
Agustin Krisna WardaniUniversity of Brawijaya2
AbinawantoAfiliasi tidak diketahui2
Lina MulyawatiBadan Pengkajian dan Penerapan Teknologi2
Maria UlfahNational Research and Innovation Agency2
Amarila MalikUniversity of Indonesia2
Fairuz Andini FatiningtyasUniversity of Indonesia2
Riswanto NapitupuluUniversity of Indonesia2
Maria UlfahNational Research and Innovation Agency2
Maria UlfahBadan Pengkajian dan Penerapan Teknologi1
Dadang SuhendarBadan Pengkajian dan Penerapan Teknologi1
Anita Kusuma FinalissariBadan Pengkajian dan Penerapan Teknologi1
Valerry Athalia PriyankaAfiliasi tidak diketahui1
Maria UlfahIPB University1
Khomaini HasanUniversitas Jenderal Achmad Yani1
I Gede Eka Perdana PutraNational Research and Innovation Agency1
Mochamad NurcholisUniversity of Brawijaya1
Muthia Rahmah DiniUniversity of Brawijaya1
Kartika Sari CendanaUniversity of Indonesia1
Dini AchnafaniNational Research and Innovation Agency1
Sunni Sofiah AniqahNational Research and Innovation Agency1
Niknik NurhayatiNational Research and Innovation Agency1
Duriya CantasinghAfiliasi tidak diketahui1
Haniyya HaniyyaBadan Pengkajian dan Penerapan Teknologi1
Phitsanu PinmaneeNational Center for Genetic Engineering and Biotechnology1
Kanokarn KocharinNational Center for Genetic Engineering and Biotechnology1
Thidarat NimchuaNational Center for Genetic Engineering and Biotechnology1
Maimunah MaimunahUniversity of Indonesia1
Ellen Fenix GunawanUniversity of Brawijaya1
Tri Yudani Mardining RarasUniversity of Brawijaya1
Mauludy Jutta AjrullahUniversity of Brawijaya1
Nadya Shafa PramestiUniversity of Brawijaya1
Sumarno Reto PrawiroUniversity of Brawijaya1
Gabriela Christy SabbthiniAfiliasi tidak diketahui1
Joko Pebrianto TrinugrohoNational Research and Innovation Agency1
Kamran MehdiUniversity of Haripur1
Obaid‐ur‐Rahman AbidHazara University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring</dc:title>
	<dc:creator xml:lang="en">HELIANTI, IS</dc:creator>
	<dc:description xml:lang="en">A functional gene containing an Open Reading Frame (ORF) encoding a ?-1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for 213 amino acids. The amino acid sequence analysis using BLAST showed that the gene has high homology (93%) with xylanase gene from Bacillus subtilis. The gene showed its function when it was subcloned into an expression vector and overexpressed in E. coli. The crude extract of the recombinant enzyme had activity for 170 U/ml at 50 oC. The result of this work showed that metagenomic approach was a powerful short cut method to obtain recombinant biocatalyst that was useful for industrial application. Key words: ?-1, 4-endoxylanase, metagenomic DNA, Pawan-Riau hot-spring </dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2009-12-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/239</dc:identifier>
	<dc:identifier>10.4308/hjb.14.2.54</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 14 No. 2 (2007): June 2007; 54</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/239/105</dc:relation>
</oai_dc:dc>