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Response of Various Tomato Genotypes to Begomovirus Infection and Its Improved Diagnostic

HAYATI Journal of Biosciences · Vol. 14 No. 3 (2009)

DOI
10.4308/hjb.14.3.93
ISSN
2086-4094, 1978-3019
Terbit
2009-12-02
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Begomovirus infection was identified from tomato growing areas in West Java (Bogor), Central Java (Boyolali), and D.I. Yogyakarta (Kaliurang). Efforts to reduce the infection among others are planting resistance varieties. This research was undertaken to evaluate 14 tomato genotypes for their response to the infection. Dot blot hybridization using nonradioactive (digoxigenin) DNA probe was employed to determine the presence of begomovirus in inoculated plants. Polymerase chain reaction-amplified product of DNA clone of tobacco leaf curl virus –Indonesia was used as a source of DNA probe. All of tomato genotypes evaluated in this study was infected separately by three strain of begomovirus (GVPSlm, GVABy, GVCBgr). Tomato genotypes Bonanza, Jelita, Safira, Permata, Presto, PSPT 8, PSPT 5B, Apel-Belgia, Karibia, Mitra, PSPT 9, Marta, and PSPT 2, showed susceptible or highly susceptible response to the three strains of begomovirus. Exception to those was shown by cv. Intan which resulted in moderate resistance when inoculated with GVCBgr although it resulted susceptible response with the other two strains. Dot-blot hybridization technique was proved to be a powerful tool to detect begomovirus infection in plants showing symptom as well as symptom-less plants. Accumulation of the virus in those plants was relatively high, except in cv. Bonanza and Apel-Belgia. Dot-blot hybridization technique using DIG-labeled DNA probe was able to detect begomovirus DNA in infected tissue up to 10-2 dilution factor. Key words: Geminivirus, hybridization, resistance, tomato

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  • IPB University
  • Universitas Halu Oleo
  • National Research and Innovation Agency
  • Organisasi Riset Pertanian dan Pangan
  • University of Brawijaya
  • Mahidol University
  • Andalas University
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Rekan penulisOrganisasiArtikel bersama
Sari NurulitaIPB University5
Kikin Hamzah MutaqinIPB University4
Damayanti BuchoriIPB University4
Widodo WidodoIPB University3
Asmar HasanUniversitas Halu Oleo2
Dewi SartiamiIPB University2
Dedi HutapeaNational Research and Innovation Agency2
Dadang DadangIPB University2
SRIANI SUJIPRIHATIAfiliasi tidak diketahui2
Bambang Tri RahardjoUniversity of Brawijaya2
MarwotoOrganisasi Riset Pertanian dan Pangan2
Tri Asmira DamayantiIPB University2
Suryo WiyonoIPB University2
Muhamad SyukurIPB University2
ORAWAN CHATCHAWANKAN PANICHAfiliasi tidak diketahui2
Muhammad TaufikUniversitas Halu Oleo1
Muhammad TaufikUniversitas Halu Oleo1
Redy GaswantoAfiliasi tidak diketahui1
Bambang Sapta PurwokoIPB University1
Lutfi AfifahIPB University1
Weerachon SawangprohMahidol University1
Netti YuliartiAndalas University1
John E. ThomasIPB University1
MUHAMMAD TAUFIKUniversitas Halu Oleo1
Gede SuastikaAfiliasi tidak diketahui1
SIENTJE MANDANG SUMARAWAfiliasi tidak diketahui1
Sri SulandariIndonesian Institute of Sciences1
RUSMILAH SUSENOAfiliasi tidak diketahui1
JUMANTO HARJOSUDARMOAfiliasi tidak diketahui1
SOEMARTONO SOSROMARSONOAfiliasi tidak diketahui1
Endang NurhayatiAfiliasi tidak diketahui1
DWI SUBEKTIAfiliasi tidak diketahui1
Tri AtmowidiIPB University1
Sjafrida ManuwotoIPB University1
Bambang SuryobrotoIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Response of Various Tomato Genotypes to Begomovirus Infection and Its Improved Diagnostic</dc:title>
	<dc:creator xml:lang="en">AIDAWATI, NOOR</dc:creator>
	<dc:creator xml:lang="en">HIDAYAT, SRI HENDRASTUTI</dc:creator>
	<dc:creator xml:lang="en">HIDAYAT, PURNAMA</dc:creator>
	<dc:creator xml:lang="en">SUSENO, RUSMILAH</dc:creator>
	<dc:creator xml:lang="en">SUJIPRIHATI, SRIANI</dc:creator>
	<dc:description xml:lang="en">Begomovirus infection was identified from tomato growing areas in West Java (Bogor), Central Java (Boyolali), and D.I. Yogyakarta (Kaliurang). Efforts to reduce the infection among others are planting resistance varieties. This research was undertaken to evaluate 14 tomato genotypes for their response to the infection. Dot blot hybridization using nonradioactive (digoxigenin) DNA probe was employed to determine the presence of begomovirus in inoculated plants. Polymerase chain reaction-amplified product of DNA clone of tobacco leaf curl virus –Indonesia was used as a source of DNA probe. All of tomato genotypes evaluated in this study was infected separately by three strain of begomovirus (GVPSlm, GVABy, GVCBgr). Tomato genotypes Bonanza, Jelita, Safira, Permata, Presto, PSPT 8, PSPT 5B, Apel-Belgia, Karibia, Mitra, PSPT 9, Marta, and PSPT 2, showed susceptible or highly susceptible response to the three strains of begomovirus. Exception to those was shown by cv. Intan which resulted in moderate resistance when inoculated with GVCBgr although it resulted susceptible response with the other two strains. Dot-blot hybridization technique was proved to be a powerful tool to detect begomovirus infection in plants showing symptom as well as symptom-less plants. Accumulation of the virus in those plants was relatively high, except in cv. Bonanza and Apel-Belgia. Dot-blot hybridization technique using DIG-labeled DNA probe was able to detect begomovirus DNA in infected tissue up to 10-2 dilution factor. Key words: Geminivirus, hybridization, resistance, tomato </dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2009-12-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/246</dc:identifier>
	<dc:identifier>10.4308/hjb.14.3.93</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 14 No. 3 (2007): September 2007; 93</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/246/112</dc:relation>
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