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Effect of Gamma Irradiation on Regeneration Capacity of Mangosteen Nodular Callus

HAYATI Journal of Biosciences · Vol. 14 No. 4 (2009)

DOI
10.4308/hjb.14.4.140
ISSN
2086-4094, 1978-3019
Terbit
2009-12-02
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

The research was conducted to determine the effect of gamma irradiation on regeneration capacity of mangosteed nodular callus. Nodular calli derived from a leaf as explants and cultured on MS medium containing combination of 2.2 µM benzilaminopurin (BAP) and 2.27 µM tidiazuron (TDZ). Nodular calli were irradiated with 0 (control) 5, 10, 15, 20, 25, 30, 35, and 40 Gy doses of gamma irradiation. After the irradiation, the calli were generated on woody plant medium (WPM), supplemented with 1.39 µM polyvinilpirolidon (PVP), 8 g.l-1 agar, 30 g.l-1 sucrose and 2.2 µM BAP concentration. Results showed that the irradiation influence the plant regeneration. Response dose of 50% (RD) that could promote the nodular calli of shoot formation was the 25 Gy while that of the shoot number per nodular calli was the 21 Gy. The shoot number irradiated with total dose 5 Gy (9.1 shoot) was higher than that of 0 Gy (8.6 shoot). Key words: plant regeneration, irradiation gamma rays, mangosteen

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  • Universitas Gadjah Mada
  • Padjadjaran University
  • IPB University
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Rekan penulisOrganisasiArtikel bersama
Y. Andi TrisyonoUniversitas Gadjah Mada2
Yuyun YuwariahPadjadjaran University1
Sheli Mustikasari DewiAfiliasi tidak diketahui1
Anne NurainiPadjadjaran University1
Wahyono WahyonoUniversitas Gadjah Mada1
Muryati MuryatiAfiliasi tidak diketahui1
Heru Rezki TanjungUniversitas Gadjah Mada1
Zulfa AzzahraUniversitas Gadjah Mada1
Anugerah Pustakawan PradiptaUniversitas Gadjah Mada1
Franciscus Xaverius WagimanUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Effect of Gamma Irradiation on Regeneration Capacity of Mangosteen Nodular Callus</dc:title>
	<dc:creator xml:lang="en">QOSIM, WARID ALI</dc:creator>
	<dc:creator xml:lang="en">PURWANTO, ROEDHY</dc:creator>
	<dc:creator xml:lang="en">WATTIMENA, GULDOF ALBERT</dc:creator>
	<dc:creator xml:lang="en">WITJAKSONO, WITJAKSONO</dc:creator>
	<dc:description xml:lang="en">The research was conducted to determine the effect of gamma irradiation on regeneration capacity of mangosteed nodular callus. Nodular calli derived from a leaf as explants and cultured on MS medium containing combination of 2.2 µM benzilaminopurin (BAP) and 2.27 µM tidiazuron (TDZ). Nodular calli were irradiated with 0 (control) 5, 10, 15, 20, 25, 30, 35, and 40 Gy doses of gamma irradiation. After the irradiation, the calli were generated on woody plant medium (WPM), supplemented with 1.39 µM polyvinilpirolidon (PVP), 8 g.l-1 agar, 30 g.l-1 sucrose and 2.2 µM BAP concentration. Results showed that the irradiation influence the plant regeneration. Response dose of 50% (RD) that could promote the nodular calli of shoot formation was the 25 Gy while that of the shoot number per nodular calli was the 21 Gy. The shoot number irradiated with total dose 5 Gy (9.1 shoot) was higher than that of 0 Gy (8.6 shoot). Key words: plant regeneration, irradiation gamma rays, mangosteen </dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2009-12-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/255</dc:identifier>
	<dc:identifier>10.4308/hjb.14.4.140</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 14 No. 4 (2007): December 2007; 140</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/255/121</dc:relation>
</oai_dc:dc>