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Diversity of Antifungal Compounds-Producing Bacillus spp. Isolated from Rhizosphere of Soybean Plant Based on ARDRA and 16S rRNA

HAYATI Journal of Biosciences · Vol. 17 No. 3 (2010)

DOI
10.4308/hjb.17.3.145
ISSN
2086-4094, 1978-3019
Terbit
2010-09-01
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Plant growth promoting rhizobacteria (PGPR) play an important role in improvement of seed germination, root development, and water utilization by plants. These rhizobacteria can stimulate plant growth directly by producing growth hormones or indirectly by producing antifungal compounds/antibiotics to suppress phytopathogenic fungi. The objective of this research was to analyze the diversity of 22 antifungal-producing rhizobacteria of Bacillus sp. isolated from rhizosphere of soybean plant based on Amplified rDNA Restriction Analysis (ARDRA) and 16S rRNA Sequence. Restriction enzymes in ARDRA analysis, HinfI, HaeIII, and RsaI were used to digest 22 16S rDNA amplified from Bacillus sp. genomes. Based on this analysis, genetic diversity of 22 Bacillus sp. producing antifungal compounds were classified into eight different groups. Moreover, six selected isolates randomly from each ARDRA group that have strong activity to suppress fungal growth were analyzed for their 16S rDNA sequences compared with reference strains. The distributions of these isolates were genetically diverse on several species of Bacillus sp. such as B. subtilis, B. cereus, and B. fusiformis. ARDRA is a reliable technique to analyze genetic diversity of Bacillus sp. community in the rhizosphere.

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Antonius SuwantoAfiliasi tidak diketahui7
Rika Indri AstutiAfiliasi tidak diketahui6
Iman RusmanaAfiliasi tidak diketahui5
Munti YuhanaIPB University3
Anja MeryandiniAfiliasi tidak diketahui3
SRI BUDIARTIAfiliasi tidak diketahui3
ESTI PUSPITASARIAfiliasi tidak diketahui3
Andreas Adhi SatyaAfiliasi tidak diketahui3
Sipriyadi SipriyadiAfiliasi tidak diketahui2
Risky Hadi WibowoAfiliasi tidak diketahui2
Maggy Thenawidjaya SuhartonoAfiliasi tidak diketahui2
Suryo WiyonoAfiliasi tidak diketahui2
Yulin LestariAfiliasi tidak diketahui2
Abdul MunifInstitut Teknologi Sepuluh Nopember2
Yepy Hardi RustamAfiliasi tidak diketahui2
Abdjad Asih NawangsihAfiliasi tidak diketahui2
Widanarni WidanarniIPB University2
. QATRUNNADAAfiliasi tidak diketahui2
Alina AkhdiyaAfiliasi tidak diketahui2
Waode MunaeniKhairun University1
Mia SetiawatiIPB University1
Iah Novi MaslahahAfiliasi tidak diketahui1
Tri Asmira DamayantiAfiliasi tidak diketahui1
HENDRA PARDEDEAfiliasi tidak diketahui1
Triadiati TriadiatiAfiliasi tidak diketahui1
ANGELIA REZTY FITRIANI SITUMORANGAfiliasi tidak diketahui1
Ari SusilowatiAfiliasi tidak diketahui1
LATIFAH KOSIM DARUSMANAfiliasi tidak diketahui1
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Martin EngelhauptAfiliasi tidak diketahui1
Hamim HamimAfiliasi tidak diketahui1
Yuli Siti FatmaAfiliasi tidak diketahui1
ARIS TJAHJOLEKSONOAfiliasi tidak diketahui1
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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Diversity of Antifungal Compounds-Producing Bacillus spp. Isolated from Rhizosphere of Soybean Plant Based on ARDRA and 16S rRNA</dc:title>
	<dc:creator xml:lang="en">WAHYUDI, ARIS TRI</dc:creator>
	<dc:creator xml:lang="en">PRASOJO, BRAMANTYO JATI</dc:creator>
	<dc:creator xml:lang="en">MUBARIK, NISA RACHMANIA</dc:creator>
	<dc:description xml:lang="en">Plant growth promoting rhizobacteria (PGPR) play an important role in improvement of seed germination, root development, and water utilization by plants. These rhizobacteria can stimulate plant growth directly by producing growth hormones or indirectly by producing antifungal compounds/antibiotics to suppress phytopathogenic fungi. The objective of this research was to analyze the diversity of 22 antifungal-producing rhizobacteria of Bacillus sp. isolated from rhizosphere of soybean plant based on Amplified rDNA Restriction Analysis (ARDRA) and 16S rRNA Sequence. Restriction enzymes in ARDRA analysis, HinfI, HaeIII, and RsaI were used to digest 22 16S rDNA amplified from Bacillus sp. genomes. Based on this analysis, genetic diversity of 22 Bacillus sp. producing antifungal compounds were classified into eight different groups. Moreover, six selected isolates randomly from each ARDRA group that have strong activity to suppress fungal growth were analyzed for their 16S rDNA sequences compared with reference strains. The distributions of these isolates were genetically diverse on several species of Bacillus sp. such as B. subtilis, B. cereus, and B. fusiformis. ARDRA is a reliable technique to analyze genetic diversity of Bacillus sp. community in the rhizosphere.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2010-09-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/1692</dc:identifier>
	<dc:identifier>10.4308/hjb.17.3.145</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 17 No. 3 (2010): September 2010; 145</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/1692/735</dc:relation>
</oai_dc:dc>