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Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.

HAYATI Journal of Biosciences · Vol. 18 No. 3 (2011)

DOI
10.4308/hjb.18.3.135
ISSN
2086-4094, 1978-3019
Terbit
2011-12-22
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.

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  • IPB University
  • Institut des Sciences de l'Evolution de Montpellier
  • Universitas Negeri Padang
  • The University of Tokyo
  • Tokyo University of Marine Science and Technology
  • National Research and Innovation Agency
  • Sriwijaya University
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Rekan penulisOrganisasiArtikel bersama
Agus Oman SudrajatIPB University3
Muhammad Agus SuprayudiIPB University3
Mia SetiawatiIPB University3
Sri NuryatiIPB University3
Ratu Siti AliahNational Research and Innovation Agency2
Komar SumantadinataIPB University2
Media Fitri Isma NugrahaAfiliasi tidak diketahui2
Jean‐Christophe AvarreInstitut des Sciences de l'Evolution de Montpellier2
Laurent PouyaudInstitut des Sciences de l'Evolution de Montpellier2
Kadarusman KadarusmanAfiliasi tidak diketahui2
Mursalim NohongAfiliasi tidak diketahui1
Insany Fitri NurqamarAfiliasi tidak diketahui1
WahdaAfiliasi tidak diketahui1
SabirAfiliasi tidak diketahui1
Andi KusumawatiAfiliasi tidak diketahui1
Abdullah SanusiAfiliasi tidak diketahui1
Amin AkbarUniversitas Negeri Padang1
LizaUniversitas Negeri Padang1
Utut WidyastutiIPB University1
Mochamad SyaifudinSriwijaya University1
RUSTIDJAAfiliasi tidak diketahui1
Irvan FaizalBadan Pengkajian dan Penerapan Teknologi1
Gemi TriastutikIPB University1
Slamet SubyaktoIPB University1
M. SASMITO JATIIPB University1
Kiyoshi FurukawaThe University of Tokyo1
Katsumi AidaThe University of Tokyo1
Ridwan AffandiIPB University1
Antonius SuwantoIPB University1
Yusminah HalaState University of Makassar1
Sri HandayaniState University of Semarang1
Ing Mok.OgintaIPB University1
Maria BintangIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en"> Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.</dc:title>
	<dc:creator xml:lang="en">ALIMUDDIN, .</dc:creator>
	<dc:creator xml:lang="en">ANDRIYANI, IRMA</dc:creator>
	<dc:creator xml:lang="en">JUNIOR, MUHAMMAD ZAIRIN</dc:creator>
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	<dc:creator xml:lang="en">CARMAN, ODANG</dc:creator>
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	<dc:subject xml:lang="en">a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2</dc:subject>
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	<dc:subject xml:lang="en">the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes</dc:subject>
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	<dc:subject xml:lang="en">amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further</dc:subject>
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	<dc:description xml:lang="en"> Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2011-12-22</dc:date>
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	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 18 No. 3 (2011): September 2011; 135</dc:source>
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