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Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P < 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.

HAYATI Journal of Biosciences · Vol. 18 No. 4 (2012)

DOI
10.4308/hjb.18.4.151
ISSN
2086-4094, 1978-3019
Terbit
2012-01-25
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P < 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.

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Bambang PurwantaraIPB University6
Cece SumantriIPB University4
AmroziAfiliasi tidak diketahui3
Ronny Rachman NoorIPB University3
Arief BoedionoIPB University3
Wahono Esthi PrasetyaningtyasIPB University2
I Ketut Mudite AdnyaneIPB University2
Yanti SulistyanaIPB University2
Muhammad AgilIPB University2
Mohamad Agus SetiadiIPB University2
Muhammad Ali ImronUniversitas Gadjah Mada2
Polmer Zaelinga SitumorangAfiliasi tidak diketahui2
Riasari Gail SianturiAfiliasi tidak diketahui2
Bambang Ngaji UtomoAfiliasi tidak diketahui2
E.D GunardiAfiliasi tidak diketahui2
Mohamad Agus SetiadiIPB University2
Amrozi AmroziIPB University1
Muhammad AgilIPB University1
Juli MeliaUniversitas Syiah Kuala1
Aryani Sismin SatyaningtijasIPB University1
Elsi RahmadhaniIPB University1
Damiana Rita EkastutiIPB University1
Ronald TariganIPB University1
Kartini ErianiUniversitas Syiah Kuala1
Sony Heru SumarsonoBandung Institute of Technology1
Al-Azhar Al-AzharUniversitas Syiah Kuala1
Dedi CandraAfiliasi tidak diketahui1
Arindina MahendraIPB University1
Aryani SatyaningtijasIPB University1
Elsi RahmadhaniIPB University1
Ronald TariganIPB University1
Damiana Rita EkastutiIPB University1
I Ketut Mudite AdnyaneIPB University1
Rimas Prathita AgustinIPB University1
HERDISAfiliasi tidak diketahui1
I.G. PutuAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P &lt; 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.</dc:title>
	<dc:creator xml:lang="en">NURBARIAH, .</dc:creator>
	<dc:creator xml:lang="en">DJUWITA, ITA</dc:creator>
	<dc:creator xml:lang="en">MOHAMAD, KUSDIANTORO</dc:creator>
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	<dc:subject xml:lang="en">therefore</dc:subject>
	<dc:subject xml:lang="en">maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either</dc:subject>
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	<dc:subject xml:lang="en">"&gt;graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. </dc:subject>
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	<dc:subject xml:lang="en">"&gt;  5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4</dc:subject>
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	<dc:subject xml:lang="en">and 26.0%</dc:subject>
	<dc:subject xml:lang="en">respectively not significantly different with those received PMSG induction</dc:subject>
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	<dc:subject xml:lang="en">and 29.9%</dc:subject>
	<dc:subject xml:lang="en">respectively. The number of oocytes and the capacity to matured</dc:subject>
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	<dc:subject xml:lang="en">PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries</dc:subject>
	<dc:subject xml:lang="en">but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.</dc:subject>
	<dc:description xml:lang="en">Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P &amp;lt; 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2012-01-25</dc:date>
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	<dc:identifier>10.4308/hjb.18.4.151</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 18 No. 4 (2011): December 2011; 151</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
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