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Isolation and Characterization of Chelonia mydas Myoglobin

HAYATI Journal of Biosciences · Vol. 19 No. 2 (2012)

DOI
10.4308/hjb.19.2.60
ISSN
2086-4094, 1978-3019
Terbit
2012-07-13
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Green sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To achieve this objective, pure green sea turtle myoglobin has to be made available. Therefore, our first task is to purify this muscle protein from green sea turtles. Skeletal muscles from 3 green sea turtle hatchlings were studied microscopically and biochemically. Microscopy observation showed a general structure of striated muscle. Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by Sephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins

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  • University of Brawijaya
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DARYL ROBERT WILLIAMSAfiliasi tidak diketahui4
ANDREW ROBERT COSSINSAfiliasi tidak diketahui4
DWIRINI RETNO GUNARTIAfiliasi tidak diketahui3
Wawan MulyawanAfiliasi tidak diketahui2
Sri Widia A. JusmanAfiliasi tidak diketahui2
RusdiAfiliasi tidak diketahui2
KURNIA NUZTIR MANTOLINIAfiliasi tidak diketahui2
Toar Jean Maurice LalisangAfiliasi tidak diketahui1
Ridho Ardhi SyaifulAfiliasi tidak diketahui1
Novi Silvia HardianyAfiliasi tidak diketahui1
Yefta MoenadjatAfiliasi tidak diketahui1
. SUBANDRATEAfiliasi tidak diketahui1
Sri HandayaniUniversity of Brawijaya1
Iman SantosoAfiliasi tidak diketahui1
HANS-JOACHIM FREISLEBENAfiliasi tidak diketahui1
HARALD HUBERAfiliasi tidak diketahui1
. ANDIAfiliasi tidak diketahui1
FERY ARDIANSYAHAfiliasi tidak diketahui1
CENMI MULYANTOAfiliasi tidak diketahui1
ZESSINDA LUTHFAAfiliasi tidak diketahui1
ROSARI SALEHAfiliasi tidak diketahui1
SERUNI KUSUMA UDYANINGSIH FREISLEBENAfiliasi tidak diketahui1
MICHAEL THOMMAfiliasi tidak diketahui1
Hanifah RahmiAfiliasi tidak diketahui1
WAHONO SUMARYONOAfiliasi tidak diketahui1
AMARILA MALIKAfiliasi tidak diketahui1
Trismilah MargonoAfiliasi tidak diketahui1
AMIEN RAMADHAN ISHAKAfiliasi tidak diketahui1
RISMA DWI UTARIAfiliasi tidak diketahui1
MELLA FERANIAAfiliasi tidak diketahui1
CHRIS ADHIYANTOAfiliasi tidak diketahui1
TAKENORI NITTAAfiliasi tidak diketahui1
AB SUSANTOAfiliasi tidak diketahui1
HATTORI YUKIOAfiliasi tidak diketahui1
YASUHIRO YAMASHIROAfiliasi tidak diketahui1
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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Isolation and Characterization of Chelonia mydas Myoglobin</dc:title>
	<dc:creator xml:lang="en">SADIKIN, MOHAMAD</dc:creator>
	<dc:creator xml:lang="en">PUSPITANINGRUM, RINI</dc:creator>
	<dc:creator xml:lang="en">WANANDI, SEPTELIA INAWATI</dc:creator>
	<dc:creator xml:lang="en">SOEGIANTO, RONDANG ROEMIATI</dc:creator>
	<dc:subject xml:lang="en">green sea turtle</dc:subject>
	<dc:subject xml:lang="en">myoglobin</dc:subject>
	<dc:subject xml:lang="en">molecular weight</dc:subject>
	<dc:subject xml:lang="en">isolation</dc:subject>
	<dc:subject xml:lang="en">characterization</dc:subject>
	<dc:description xml:lang="en">Green sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To achieve this objective, pure green sea turtle myoglobin has to be made available. Therefore, our first task is to purify this muscle protein from green sea turtles. Skeletal muscles from 3 green sea turtle hatchlings were studied microscopically and biochemically. Microscopy observation showed a general structure of striated muscle. Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by Sephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2012-07-13</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/5305</dc:identifier>
	<dc:identifier>10.4308/hjb.19.2.60</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 19 No. 2 (2012): June 2012; 60</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/5305/3701</dc:relation>
</oai_dc:dc>