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Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis

HAYATI Journal of Biosciences · Vol. 19 No. 2 (2012)

DOI
10.4308/hjb.19.2.93
ISSN
2086-4094, 1978-3019
Terbit
2012-07-13
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Tempe fermentation involved complex microbial communities which are only revealed partially through culture dependent methods. Culture-independent methods would be potential to unravel this complex microbial fermentation. Appropriate DNA extraction is an essential tool to obtain reliable data from culture independent method. In this study, we employed two commercial DNA extraction methods to find the best one for microbial community characterization employing amplified ribosomal intergenic spacer analysis (ARISA). Our result showed that PowerFood Microbial DNA Isolation Kit-MOBIO (PFMDIK) is an excellent method for microbial DNA extraction from tempe. It gave high quantity and quality of DNA suitable for PCR amplification of 16S-23S rRNA intergenic spacer to yield a diverse and reproducible ARISA profile.

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  • IPB University
  • Universitas Pattimura
  • University of Indonesia
  • Hasanuddin University
  • Universitas Sumatera Utara
  • University of Bengkulu
  • Universitas Islam Negeri Maulana Malik Ibrahim
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Rekan penulisOrganisasiArtikel bersama
Nisa Rachmania MubarikIPB University8
Esti PuspitasariIPB University4
Anja MeryandiniIPB University4
Aris Tri WahyudiIPB University4
Yulin LestariIPB University4
Rika Indri AstutiIPB University4
Dwi SuryantoUniversitas Sumatera Utara3
Andreas Adhi SatyaAfiliasi tidak diketahui3
Cece SumantriIPB University3
Neviaty Putri ZamaniIPB University2
Risky Hadi WibowoAfiliasi tidak diketahui2
Sipriyadi SipriyadiUniversity of Bengkulu2
Hamim HamimUniversitas Islam Negeri Maulana Malik Ibrahim2
Widanarni WidanarniIPB University2
Amarila MalikUniversity of Indonesia2
Bibiana W. LayIPB University2
Alina AkhdiyaIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development2
Bambang PurwantaraIPB University2
Maria Indah PurnamasariMontana State University2
Yepy H. RustamAfiliasi tidak diketahui2
Ridwan AffandiIPB University2
Andi HaerulHasanuddin University1
Syafyudin YusufHasanuddin University1
SuharsonoNational Research and Innovation Agency1
Maggy Thenawidjaya SuhartonoIPB University1
Epa PaujiahSunan Gunung Djati State Islamic University Bandung1
Dian HendrayantiUniversity of Indonesia1
Dwi Andreas SantosaIPB University1
Alice Chaela LethulurUniversitas Pattimura1
Edwin Thomas ApituleyUniversitas Pattimura1
ALINA AKHADIYAAfiliasi tidak diketahui1
Nurul AiniIPB University1
Walter R. ErdelenIPB University1
Muhammad ZairinIPB University1
Yusminah HalaState University of Makassar1
Sukenda SukendaIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis</dc:title>
	<dc:creator xml:lang="en">SEUMAHU, CECILIA ANNA</dc:creator>
	<dc:creator xml:lang="en">SUWANTO, ANTONIUS</dc:creator>
	<dc:creator xml:lang="en">RUSMANA, IMAN</dc:creator>
	<dc:creator xml:lang="en">SOLIHIN, DEDY DURYADI</dc:creator>
	<dc:subject xml:lang="en">ARISA profile</dc:subject>
	<dc:subject xml:lang="en">DNA extraction</dc:subject>
	<dc:subject xml:lang="en">tempe microbial community</dc:subject>
	<dc:description xml:lang="en">Tempe fermentation involved complex microbial communities which are only revealed partially through culture dependent methods. Culture-independent methods would be potential to unravel this complex microbial fermentation. Appropriate DNA extraction is an essential tool to obtain reliable data from culture independent method. In this study, we employed two commercial DNA extraction methods to find the best one for microbial community characterization employing amplified ribosomal intergenic spacer analysis (ARISA). Our result showed that PowerFood Microbial DNA Isolation Kit-MOBIO (PFMDIK) is an excellent method for microbial DNA extraction from tempe. It gave high quantity and quality of DNA suitable for PCR amplification of 16S-23S rRNA intergenic spacer to yield a diverse and reproducible ARISA profile.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2012-07-13</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/5310</dc:identifier>
	<dc:identifier>10.4308/hjb.19.2.93</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 19 No. 2 (2012): June 2012; 93</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/5310/3706</dc:relation>
</oai_dc:dc>