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Yeast Isolation for Bioethanol Production

HAYATI Journal of Biosciences · Vol. 19 No. 3 (2012)

DOI
10.4308/hjb.19.3.145
ISSN
2086-4094, 1978-3019
Terbit
2012-10-15
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.

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  • IPB University
  • Universitas Sumatera Utara
  • National Research and Innovation Agency
  • Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development
  • Sriwijaya University
  • University of Göttingen
  • Universiti Putra Malaysia
  • Afiliasi tidak diketahui
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Rika Indri AstutiIPB University4
Antonius SuwantoIPB University3
Aris Tri WahyudiIPB University3
Dwi SuryantoUniversitas Sumatera Utara2
YopiAfiliasi tidak diketahui2
Yulin LestariIPB University2
Apon Zaenal MustopaNational Research and Innovation Agency2
Dimas AndriantoIPB University2
Aprilia NaomiIPB University2
FERY MUTIAIPB University2
M. EDY SOFIYANTOIPB University2
Nur RichanaIPB University2
Vestika Iskawati Wahidul HijahIPB University2
Yaya RukayadiUniversiti Putra Malaysia2
Eka putriIPB University2
Deden SaprudinIPB University1
Prima Agung PrihandonoIPB University1
Alina AkhdiyaIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development1
Trio HendarwinAfiliasi tidak diketahui1
Ono SuparnoIPB University1
Akhmad Endang Zainal HasanIPB University1
Djumali MangunwidjajaIPB University1
Agus SetiyonoIPB University1
Kurrataa’yunIPB University1
AriandiIPB University1
Munti YuhanaIPB University1
Marini WijayantiSriwijaya University1
Martin EngelhauptUniversity of Göttingen1
Ade IrmaAfiliasi tidak diketahui1
Bedah RupaedahAfiliasi tidak diketahui1
Nurfadhilla RahmadhaniIPB University1
Mazidah Noer InayahIPB University1
Arina Amalia PutriIPB University1
Alfred MichaelAfiliasi tidak diketahui1
Maria SugihartiAfiliasi tidak diketahui1
Muhammad Fadhil FathiahIPB University1
RamlanAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Yeast Isolation for Bioethanol Production</dc:title>
	<dc:creator xml:lang="en">RURIANI, EKA</dc:creator>
	<dc:creator xml:lang="en">SUNARTI, TITI CANDRA</dc:creator>
	<dc:creator xml:lang="en">MERYANDINI, ANJA</dc:creator>
	<dc:subject xml:lang="en">yeast</dc:subject>
	<dc:subject xml:lang="en">glucose-xylose substrate</dc:subject>
	<dc:subject xml:lang="en">ethanol</dc:subject>
	<dc:description xml:lang="en">We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2012-10-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/5632</dc:identifier>
	<dc:identifier>10.4308/hjb.19.3.145</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 19 No. 3 (2012): September 2012; 145</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/5632/4271</dc:relation>
</oai_dc:dc>