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Archaeal Life on Tangkuban Perahu- Sampling and Culture Growth in Indonesian Laboratories

HAYATI Journal of Biosciences · Vol. 19 No. 3 (2012)

DOI
10.4308/hjb.19.3.150
ISSN
2086-4094, 1978-3019
Terbit
2012-10-15
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

The aim of the expedition to Tangkuban Perahu, West Java was to obtain archaeal samples from the solfatara fields located in Domas crater. This was one of the places, where scientists from the University of Regensburg Germany had formerly isolated Indonesian archaea, especially Thermoplasma and Sulfolobus species but not fully characterized. We collected five samples from mud holes with temperatures from 57 to 88 oC and pH of 1.5-2. A portion of each sample was grown at the University of Regensburg in modified Allen’s medium at 80 oC. From four out of five samples enrichment cultures were obtained, autotrophically on elemental sulphur and heterotrophically on sulfur and yeast extract; electron micrographs are presented. In the laboratories of Universitas Indonesia the isolates were cultured at 55-60 oC in order to grow tetraetherlipid synthesizing archaea, both Thermoplasmatales and Sulfolobales. Here, we succeeded to culture the same type of archaeal cells, which had been cultured in Regensburg, probably a Sulfolobus species and in Freundt’s medium, Thermoplasma species. The harvested cells are documented by phase contrast microscope equipped with a digital camera. Our next steps will be to further characterize genetically the cultured cells from Tangkuban Perahu isolates.

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Rekan penulisOrganisasiArtikel bersama
Riris Istighfari JenieAfiliasi tidak diketahui6
Mohamad SyaefudinAfiliasi tidak diketahui4
Diah Vitri WidayantiAfiliasi tidak diketahui4
Ratna Asmah SusidartiAfiliasi tidak diketahui4
Neli PurwaniAfiliasi tidak diketahui3
Mohamad SadikinAfiliasi tidak diketahui3
RINI PUSPITANINGRUMAfiliasi tidak diketahui3
RONDANG ROEMIATI SOEGIANTOAfiliasi tidak diketahui3
Edy MeiyantoAfiliasi tidak diketahui3
Zalinar UdinAfiliasi tidak diketahui3
Dwi AstutiAfiliasi tidak diketahui2
Tri Eko AgustiningrumAfiliasi tidak diketahui2
Sudarwoto SudarwotoAfiliasi tidak diketahui2
Nurul YuhanafiaAfiliasi tidak diketahui2
Virgiawan Adi KristiantoAfiliasi tidak diketahui2
Cici DarsihAfiliasi tidak diketahui2
Wega TrisunaryantiAfiliasi tidak diketahui2
TriyonoAfiliasi tidak diketahui2
Aan KurniawanNational Research and Innovation Agency2
Syarifah NurainiAfiliasi tidak diketahui2
Ani Budi AstutiUniversity of Brawijaya1
Suci AstutikUniversity of Brawijaya1
Nur Silviyah RahmiUniversity of Brawijaya1
Alifiandi Rafi MuhammadUniversity of Brawijaya1
Ulfah MaisarohUniversity of Brawijaya1
Kismi MubarokahAfiliasi tidak diketahui1
Harianingsih HarianingsihAfiliasi tidak diketahui1
Maharani KusumaningrumAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Archaeal Life on Tangkuban Perahu- Sampling and Culture Growth in Indonesian Laboratories</dc:title>
	<dc:creator xml:lang="en">HANDAYANI, SRI</dc:creator>
	<dc:creator xml:lang="en">SANTOSO, IMAN</dc:creator>
	<dc:creator xml:lang="en">FREISLEBEN, HANS-JOACHIM</dc:creator>
	<dc:creator xml:lang="en">HUBER, HARALD</dc:creator>
	<dc:creator xml:lang="en">ANDI, .</dc:creator>
	<dc:creator xml:lang="en">ARDIANSYAH, FERY</dc:creator>
	<dc:creator xml:lang="en">MULYANTO, CENMI</dc:creator>
	<dc:creator xml:lang="en">LUTHFA, ZESSINDA</dc:creator>
	<dc:creator xml:lang="en">SALEH, ROSARI</dc:creator>
	<dc:creator xml:lang="en">FREISLEBEN, SERUNI KUSUMA  UDYANINGSIH</dc:creator>
	<dc:creator xml:lang="en">WANANDI, SEPTELIA INAWATI</dc:creator>
	<dc:creator xml:lang="en">THOMM, MICHAEL</dc:creator>
	<dc:subject xml:lang="en">Archaea</dc:subject>
	<dc:subject xml:lang="en">Sulfolobus</dc:subject>
	<dc:subject xml:lang="en">Thermoplasma</dc:subject>
	<dc:subject xml:lang="en">Tangkuban Perahu</dc:subject>
	<dc:subject xml:lang="en">Indonesian volcanoes</dc:subject>
	<dc:subject xml:lang="en">tetraether lipid</dc:subject>
	<dc:description xml:lang="en">The aim of the expedition to Tangkuban Perahu, West Java was to obtain archaeal samples from the solfatara fields located in Domas crater. This was one of the places, where scientists from the University of Regensburg Germany had formerly isolated Indonesian archaea, especially Thermoplasma and Sulfolobus species but not fully characterized. We collected five samples from mud holes with temperatures from 57 to 88 oC and pH of 1.5-2. A portion of each sample was grown at the University of Regensburg in modified Allen’s medium at 80 oC. From four out of five samples enrichment cultures were obtained, autotrophically on elemental sulphur and heterotrophically on sulfur and yeast extract; electron micrographs are presented. In the laboratories of Universitas Indonesia the isolates were cultured at 55-60 oC in order to grow tetraetherlipid synthesizing archaea, both Thermoplasmatales and Sulfolobales. Here, we succeeded to culture the same type of archaeal cells, which had been cultured in Regensburg, probably a Sulfolobus species and in Freundt’s medium, Thermoplasma species. The harvested cells are documented by phase contrast microscope equipped with a digital camera. Our next steps will be to further characterize genetically the cultured cells from Tangkuban Perahu isolates.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2012-10-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/5639</dc:identifier>
	<dc:identifier>10.4308/hjb.19.3.150</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 19 No. 3 (2012): September 2012; 150</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/5639/4272</dc:relation>
</oai_dc:dc>