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Liver Histophatological Studies of Mice (Mus musculus sp.) Infected With Gamma Rays Irradiated Plasmodium berghei Strains Anka

HAYATI Journal of Biosciences · Vol. 20 No. 3 (2013)

DOI
10.4308/hjb.20.3.144
ISSN
2086-4094, 1978-3019
Terbit
2013-09-30
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Malaria is a serious global disease and a leading cause of morbidity and mortality in tropical and sub-tropical countries. Radiation attenuated vaccines have been demonstrated to be an effective means of controlling certain parasitic infections such as malaria. Our previous research showed that irradiation dose of 150-175 Gy to Plasmodium berghei were enable to suppress the infected erythrocyte cells and irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on parasitemia index. Aim of this research was to investigate the most suitable irradiation dose to attenuated P. berghei based on the the histological changes in the liver mouse malaria model. Histological changes in mice liver because of Plasmodium is congestion in the sinusoids and masses of hemozoin. Fourty Swiss mice were randomly assigned to four equal groups. First group of mice were inoculated with the unirradiated malaria parasites as a positive control while negative control group represented uninfected normal control. The third group of mice was inoculated with the irradiated malaria parasites with dose 175 Gy. Last group were inoculated with the irradiated malaria parasites with dose 150 Gy. Mice were sacrificed at day 6, 8, 12, 16, 20, and 24 post inoculation for liver histological study. Their livers processed for histomorphometric assessment of sinusoidal area, haemozoin area and hepatosomatic index as a quantitative measure of altered morphology. Hepatic sinusoidal area in mice infected with the unirradiated malaria parasites increased by 104% in day 24 post inoculated compared to day 6 post inoculated, whereas a smaller increase of 46% occurred in mice that were inoculated with the irradiated malaria parasites with dose 150 Gy. The difference between positive control and 150 Gy group was significant (P 0.05). For hepatosomatic index (HSI) at day 24 post inoculation the HSI value in group 150 Gy (5.33) was the smallest compared to other groups. Based on our research it can be concluded that irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on assessment of sinusoidal area, and HSI value at 24 day post inoculation.

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  • National Nuclear Energy Agency of Indonesia
  • Hirosaki University
  • University of Indonesia
  • State University of Semarang
  • National Research and Innovation Agency
  • Dharmais Cancer Hospital
  • Universitas Gadjah Mada
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Rekan penulisOrganisasiArtikel bersama
Devita TetrianaNational Nuclear Energy Agency of Indonesia16
Mukh SyaifudinNational Nuclear Energy Agency of Indonesia15
Sofiati PurnamiNational Nuclear Energy Agency of Indonesia13
Darlina DarlinaNational Nuclear Energy Agency of Indonesia7
Harry Nugroho Eko SurniyantoroNational Nuclear Energy Agency of Indonesia6
Yanti LusiyantiNational Nuclear Energy Agency of Indonesia6
Teja KisnantoNational Nuclear Energy Agency of Indonesia6
Wiwin MailanaNational Nuclear Energy Agency of Indonesia5
Eko PudjadiNational Nuclear Energy Agency of Indonesia5
Nastiti RahajengNational Nuclear Energy Agency of Indonesia4
Ikuo KashiwakuraHirosaki University4
Masnelli LubisNational Nuclear Energy Agency of Indonesia3
H. DateHokkaido University3
T. IshidaThe University of Tokyo3
Silvia Tri WidyaningtyasUniversity of Indonesia3
I.K.H. BasriNational Nuclear Energy Agency of Indonesia2
Yustinus Ulung AnggraitoState University of Semarang2
Amalia Dyah PratiwiState University of Semarang2
Darlina YusufNational Nuclear Energy Agency of Indonesia2
K. D. PurwantiNational Nuclear Energy Agency of Indonesia2
Ryuji OkazakiUniversity of Occupational and Environmental Health Japan2
Zubaidah AlatasNational Nuclear Energy Agency of Indonesia1
Viria Agesti SuvifanNational Nuclear Energy Agency of Indonesia1
M. R. A. GaniDharmais Cancer Hospital1
I.K. Hasan BasriNational Research and Innovation Agency1
Ayu Rosemeilia DewiDharmais Cancer Hospital1
V. A. SuvivanNational Research and Innovation Agency1
M. YoshidaHirosaki University1
Ahmad Hamim SadewaUniversitas Gadjah Mada1
Pramudji HastutiUniversitas Gadjah Mada1
S. SimbolonAfiliasi tidak diketahui1
Supriyono SupriyonoNational Nuclear Energy Agency of Indonesia1
Aryadi AryadiAfiliasi tidak diketahui1
Sultana MH FaradzDiponegoro University1
Aroem NaroeniUniversity of Indonesia1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Liver Histophatological Studies of Mice (Mus musculus sp.) Infected With Gamma Rays Irradiated Plasmodium berghei Strains Anka</dc:title>
	<dc:creator xml:lang="en">RAHARDJO, TUR</dc:creator>
	<dc:creator xml:lang="en">NURHAYATI, SITI</dc:creator>
	<dc:creator xml:lang="en">RAMADHANI, DWI</dc:creator>
	<dc:subject xml:lang="en">gamma rays</dc:subject>
	<dc:subject xml:lang="en">histopathology</dc:subject>
	<dc:subject xml:lang="en">liver</dc:subject>
	<dc:subject xml:lang="en">Mus musculus</dc:subject>
	<dc:subject xml:lang="en">Plasmodium berghei</dc:subject>
	<dc:description xml:lang="en">Malaria is a serious global disease and a leading cause of morbidity and mortality in tropical and sub-tropical countries. Radiation attenuated vaccines have been demonstrated to be an effective means of controlling certain parasitic infections such as malaria. Our previous research showed that irradiation dose of 150-175 Gy to Plasmodium berghei were enable to suppress the infected erythrocyte cells and irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on parasitemia index. Aim of this research was to investigate the most suitable irradiation dose to attenuated P. berghei based on the the histological changes in the liver mouse malaria model. Histological changes in mice liver because of Plasmodium is congestion in the sinusoids and masses of hemozoin. Fourty Swiss mice were randomly assigned to four equal groups. First group of mice were inoculated with the unirradiated malaria parasites as a positive control while negative control group represented uninfected normal control. The third group of mice was inoculated with the irradiated malaria parasites with dose 175 Gy. Last group were inoculated with the irradiated malaria parasites with dose 150 Gy. Mice were sacrificed at day 6, 8, 12, 16, 20, and 24 post inoculation for liver histological study. Their livers processed for histomorphometric assessment of sinusoidal area, haemozoin area and hepatosomatic index as a quantitative measure of altered morphology. Hepatic sinusoidal area in mice infected with the unirradiated malaria parasites increased by 104% in day 24 post inoculated compared to day 6 post inoculated, whereas a smaller increase of 46% occurred in mice that were inoculated with the irradiated malaria parasites with dose 150 Gy. The difference between positive control and 150 Gy group was significant (P &lt; 0.05).  The highest average haemozoin area in all days post inoculation was in positive control whereas a smaller occurred in mice that were inoculated with the irradiated malaria parasites with dose 175 Gy, but the different was not significant (P &gt; 0.05). For hepatosomatic index (HSI) at day 24 post inoculation the HSI value in group 150 Gy (5.33) was the smallest compared to other groups. Based on our research it can be concluded that irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on assessment of sinusoidal area, and HSI value at 24 day post inoculation.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2013-09-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/7064</dc:identifier>
	<dc:identifier>10.4308/hjb.20.3.144</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 20 No. 3 (2013): September 2013; 144</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/7064/5483</dc:relation>
</oai_dc:dc>