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In Vitro Growth and Rooting of Mangosteen (Garcinia mangostana L.) on Medium with Different Concentrations of Plant Growth Regulator

HAYATI Journal of Biosciences · Vol. 21 No. 4 (2014)

DOI
10.4308/hjb.21.4.151
ISSN
2086-4094, 1978-3019
Terbit
2014-12-01
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Propagation of mangosteen is challenging for many reasons, including limited seed set, slow rate of seedling growth, and difficulty with root formations. The objective of this research was to find the best combination of medium and plant growth regulator for in vitro growth and rooting of mangosteeen seed. Various types of explant (a whole seed; seed divided into 2, 3, and 4 cross sections; seed divided into 2, 3, and 4 longitudinal sections) were treated with five concentrations of benzyl amino purine (BAP; 0, 2.5, 5, 7.5, 10 mg/L) for shoot induction in ½ Nitrogen (N) Murashige and Skoog (MS) medium. The shoots were rooted on MS and woody plant medium (WPM) media with several combinations of indole butyric acid (IBA) and naphtalene acetic acid (NAA). Treatments for root induction were applied as follows: (i) low dose, given during induction of rooting, (ii) soaking the base of the shoots in medium treated with a high dose of auxin for 5 days, and then growing the shoots in MS ½ N with 1 mg/L NAA + 1 mg/L BAP medium. Our result show that BAP positively affected mangosteen bud growth. The best medium for mangosteen shoot regeneration was found to be MS ½ N + 5 mg/L BAP. This medium induced the highest number of shoots from the seed explant cut into four cross sections. We found the best medium to induce in vitro rooting of mangosteen shoot was MS ½ N + 3 mg/L indole butiric acid (IBA) + 4 mg/L NAA medium. Some treatment negatively affected growth. Soaking the mangosteen shoot base in a medium with an overly high dose of auxin seemed to disrupt and inhibit growth of the mangosteen shoot.

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  • State University of Medan
  • IPB University
  • National Research and Innovation Agency
  • Hasanuddin University
  • Universitas Islam Negeri Ar-Raniry
  • Nara Institute of Science and Technology
  • Universitas Sumatera Utara
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Rekan penulisOrganisasiArtikel bersama
Neviaty Putri ZamaniIPB University2
Ramlan SilabanState University of Medan1
Lisa IndrayaniAfiliasi tidak diketahui1
Hendro PranotoState University of Medan1
Martina Asiati NapitupuluState University of Medan1
Andi HaerulHasanuddin University1
Syafyudin YusufHasanuddin University1
Dedy Duryadi SolihinIPB University1
Bella Cronika PasaribuState University of Medan1
Samsul KamalUniversitas Islam Negeri Ar-Raniry1
Cicik SurianiState University of Medan1
Syahmi EdiState University of Medan1
Eko ParasetyaState University of Medan1
Nurlia ZaharaUniversitas Islam Negeri Ar-Raniry1
Ayu Putri NingsihState University of Medan1
Armaniar ArmaniarUniversitas Pembangunan Panca Budi Medan1
Juliarni JuliarniUniversitas Rokania1
Dorly DorlyAfiliasi tidak diketahui1
Soekisman TjitrosemitoSoutheast Asian Regional Centre for Tropical Biology1
Didy SopandieIPB University1
Radite TistamaIPB University1
Utut WidyastutiIPB University1
Akiho YokotaNara Institute of Science and Technology1
Kinya AkashiNara Institute of Science and Technology1
Dietriech Geoffrey BengenAfiliasi tidak diketahui1
Ofri JohanAfiliasi tidak diketahui1
Michael John SweetAfiliasi tidak diketahui1
Anja MeryandiniIPB University1
Sri KoerniatiNational Research and Innovation Agency1
Dea ArmitaIPB University1
Yusmani PrayogoNational Research and Innovation Agency1
Yuanita RachmawatiUIN Sunan Ampel Surabaya1
Eko PrasetyaState University of Medan1
Lazuardi LazuardiState University of Medan1
Yusnaeni YusufState University of Makassar1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">In Vitro Growth and Rooting of Mangosteen (Garcinia mangostana L.) on Medium with Different Concentrations of Plant Growth Regulator</dc:title>
	<dc:creator xml:lang="en">HARAHAP, FAUZIYAH</dc:creator>
	<dc:creator xml:lang="en">POERWANTO, ROEDHY</dc:creator>
	<dc:creator xml:lang="en">SUHARSONO, .</dc:creator>
	<dc:creator xml:lang="en">SURIANI, CICIK</dc:creator>
	<dc:creator xml:lang="en">RAHAYU, SUCI</dc:creator>
	<dc:subject xml:lang="en">mangosteen</dc:subject>
	<dc:subject xml:lang="en">tissue culture</dc:subject>
	<dc:subject xml:lang="en">auxin</dc:subject>
	<dc:subject xml:lang="en">cytokinin</dc:subject>
	<dc:description xml:lang="en">Propagation of mangosteen is challenging for many reasons, including limited seed set, slow rate of seedling growth, and difficulty with root formations. The objective of this research was to find the best combination of medium and plant growth regulator for in vitro growth and rooting of mangosteeen seed. Various types of explant (a whole seed; seed divided into 2, 3, and 4 cross sections; seed divided into 2, 3, and 4 longitudinal sections) were treated with five concentrations of benzyl amino purine (BAP; 0, 2.5, 5, 7.5, 10 mg/L) for shoot induction in ½ Nitrogen (N) Murashige and Skoog (MS) medium. The shoots were rooted on MS and woody plant medium (WPM) media with several combinations of indole butyric acid (IBA) and naphtalene acetic acid (NAA). Treatments for root induction were applied as follows: (i) low dose, given during induction of rooting, (ii) soaking the base of the shoots in medium treated with a high dose of auxin for 5 days, and then growing the shoots in MS ½ N with 1 mg/L NAA +  1 mg/L BAP medium. Our result show that BAP positively affected mangosteen bud growth. The best medium for mangosteen shoot regeneration was found to be  MS ½ N  + 5 mg/L BAP. This medium induced  the highest number of shoots from the seed explant cut into four cross sections. We found the best medium to induce in vitro rooting of mangosteen shoot was MS ½ N + 3 mg/L indole butiric acid (IBA) + 4 mg/L NAA medium. Some treatment negatively affected growth. Soaking the mangosteen shoot base in a medium with an overly high dose of auxin seemed to disrupt and inhibit growth of the mangosteen shoot.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2014-12-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/8978</dc:identifier>
	<dc:identifier>10.4308/hjb.21.4.151</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 21 No. 4 (2014): December 2014; 151</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/8978/7028</dc:relation>
</oai_dc:dc>