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Characterization of Bacteriophage Specific to Bacillus pumilus from Ciapus River in Bogor, West Java, Indonesia

HAYATI Journal of Biosciences · Vol. 22 No. 1 (2015)

DOI
10.4308/hjb.22.1.27
ISSN
2086-4094, 1978-3019
Terbit
2015-01-02
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Bacillus pumilus is a spore-forming bacteria that is rod-shaped, gram positive, and aerobic. B. pumilus produced pumilacidins, known to have toxic effects on epithelial cells. Antibiotics were usually used to treat the disease caused by bacteria. Antibiotic typing test of B. pumilus indigenous from sewage water showed that this isolate was resistant to ampicillin and clindamycin. An alternative way was by application of bacteriophages as biocontrol agents to reduce B. pumilus in environment. The aim of this study were to isolate and characterize B. pumilus bacteriophage isolated from Ciapus River in Bogor, West Java. Bacteriophages infecting B. pumilus were isolated from river water using the double agar overlay method. Phages were defined by plaque morphology, structure, host range, and characteristic of molecular weight protein phage. Phage FBa1, FBa2, and FBa3 had narrow host range and they were specific for infecting B. pumilus. Electron microscope observation showed that phage FBa1 had icosahedral head without tail (166.67 nm in diameter), so it is called phage-like particles. Characterization of phage FBa1 by SDS-PAGE showed five proteins band. Molecular weight of FBa1 proteins was 70.9, 54.9, 33.8, 28.3, and 21.4 kDa.

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  • IPB University
  • Universitas Gadjah Mada
  • University of Bengkulu
  • Universitas Islam Negeri Maulana Malik Ibrahim
  • Universitas Pattimura
  • Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development
  • University of Indonesia
  • Organisasi lain
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Rekan penulisOrganisasiArtikel bersama
Nisa Rachmania MubarikIPB University6
Antonius SuwantoIPB University4
Rika Indri AstutiIPB University4
Yulin LestariIPB University3
Liga InsaniAfiliasi tidak diketahui2
Diah Ayu Satyari UtamiAfiliasi tidak diketahui2
Risky Hadi WibowoAfiliasi tidak diketahui2
Sipriyadi SipriyadiUniversity of Bengkulu2
Hamim HamimUniversitas Islam Negeri Maulana Malik Ibrahim2
Bambang SuryobrotoIPB University2
Alex HartanaIPB University2
Dedy Duryadi SolihinIPB University2
Cecilia Anna SeumahuUniversitas Pattimura2
Alina AkhdiyaIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development2
Aris Tri WahyudiIPB University2
IlhamAfiliasi tidak diketahui1
Annisa Khairani ArasAfiliasi tidak diketahui1
Mohsan AbroriAfiliasi tidak diketahui1
I Nyoman SudiarsaAfiliasi tidak diketahui1
Arie KiswantoAfiliasi tidak diketahui1
Andina Chairun NisaAfiliasi tidak diketahui1
Diklawati JatayuAfiliasi tidak diketahui1
Amiqatul FikriyahAfiliasi tidak diketahui1
Muhammad Chaidir UnduAfiliasi tidak diketahui1
Maggy Thenawidjaya SuhartonoIPB University1
Dian HendrayantiUniversity of Indonesia1
Dwi Andreas SantosaIPB University1
ALINA AKHADIYAAfiliasi tidak diketahui1
Siti Muslimah WidyastutiUniversitas Gadjah Mada1
Sebastian MarginoUniversitas Gadjah Mada1
Tetri WidiyaniSebelas Maret University1
Elda Irma Jeanne Joice KawulurUniversitas Papua1
Dewi SartikaLampung University1
Mirnawati Bachrum SudarwantoIPB University1
Dondin SajuthiIPB University1
Susan SokaAtma Jaya Catholic University of Indonesia1
Diah IskandriatiIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Characterization of Bacteriophage Specific to Bacillus pumilus from Ciapus River in Bogor, West Java, Indonesia</dc:title>
	<dc:creator xml:lang="en">KUSMIATUN, ANIK</dc:creator>
	<dc:creator xml:lang="en">RUSMANA, IMAN</dc:creator>
	<dc:creator xml:lang="en">BUDIARTI, SRI</dc:creator>
	<dc:subject xml:lang="en">Bacillus pumilus</dc:subject>
	<dc:subject xml:lang="en">bacteriophage</dc:subject>
	<dc:subject xml:lang="en">food poisoning</dc:subject>
	<dc:description xml:lang="en">Bacillus pumilus is a spore-forming bacteria that is rod-shaped, gram positive, and aerobic. B. pumilus produced pumilacidins, known to have toxic effects on epithelial cells. Antibiotics were usually used to treat the disease caused by bacteria. Antibiotic typing test of B. pumilus indigenous from sewage water showed that this isolate was resistant to ampicillin and clindamycin. An alternative way was by application of bacteriophages as biocontrol agents to reduce B. pumilus in environment. The aim of this study were to isolate and characterize B. pumilus bacteriophage isolated from Ciapus River in Bogor, West Java. Bacteriophages infecting B. pumilus were isolated from river water using the double agar overlay method. Phages were defined by plaque morphology, structure, host range, and characteristic of molecular weight protein phage. Phage FBa1, FBa2, and FBa3 had narrow host range and they were specific for infecting B. pumilus. Electron microscope observation showed that phage FBa1 had icosahedral head without tail (166.67 nm in diameter), so it is called phage-like particles. Characterization of phage FBa1 by SDS-PAGE showed five proteins band. Molecular weight of FBa1 proteins was 70.9, 54.9, 33.8, 28.3, and 21.4 kDa.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2015-01-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/9217</dc:identifier>
	<dc:identifier>10.4308/hjb.22.1.27</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 22 No. 1 (2015): January 2015; 27</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/9217/pdf</dc:relation>
</oai_dc:dc>