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Analysis of Intestinal Mucosal Immunoglobulin A in Sprague Dawley Rats Supplemented with Tempeh

HAYATI Journal of Biosciences · Vol. 22 No. 1 (2015)

DOI
10.4308/hjb.22.1.48
ISSN
2086-4094, 1978-3019
Terbit
2015-01-02
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Tempeh is a well-known Indonesian fermented food made from soybean. During the fermentation process, microorganisms play an important role in the flavor, texture, and nutritional quality of tempeh. Tempeh has been show to have immuno-modulatory and immune-stimulating properties that may also be caused by the microorganisms in tempeh as they interact between the microbial population in the intestinal tract. The objective of this study was to quantify IgA gene expression at both the transcription and translation levels in Sprague Dawley (SD) rats supplemented with tempeh. A total of 6 female SD rats were divided into 3 groups of 2 rats. The first group was the control and was fed a standard diet without tempeh. The second- and third group were fed with a standard diet supplemented with raw and cooked tempeh, respectively. Ileum tissue samples were collected after tempeh supplementation for 28 days. RNA was extracted from ileum samples, and measurement of IgA gene expression was further analyzed using semi quantitative real-time PCR. The concentration of IgA protein was quantified from ileum lysate using the half sandwich ELISA method. IgA gene expressions in rats supplemented with raw, and with cooked tempeh, were 1.18 and 1.17 fold higher, respectively, compared to the control group. Moreover, IgA protein secretion levels also increased 2.46 and 2.08 fold, respectively, compared to the control group. The result of this study indicates that both raw and cooked tempeh may stimulate IgA secretion, and also that both viable and non-viable microorganisms might stimulate IgA gene expression.

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  • IPB University
  • Atma Jaya Catholic University of Indonesia
  • University of Indonesia
  • Universitas Sumatera Utara
  • University of Bengkulu
  • Universitas Islam Negeri Maulana Malik Ibrahim
  • Universitas Pattimura
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Rekan penulisOrganisasiArtikel bersama
Nisa Rachmania MubarikIPB University8
Esti PuspitasariIPB University4
Anja MeryandiniIPB University4
Aris Tri WahyudiIPB University4
Dedy Duryadi SolihinIPB University4
Cecilia Anna SeumahuUniversitas Pattimura4
Rika Indri AstutiIPB University4
Dwi SuryantoUniversitas Sumatera Utara3
Bambang PurwantaraIPB University3
Andreas Adhi SatyaAfiliasi tidak diketahui3
Yulin LestariIPB University3
Fitriya N. DewiIPB University3
Risky Hadi WibowoAfiliasi tidak diketahui2
Sipriyadi SipriyadiUniversity of Bengkulu2
Hamim HamimUniversitas Islam Negeri Maulana Malik Ibrahim2
Widanarni WidanarniIPB University2
Amarila MalikUniversity of Indonesia2
Bibiana W. LayIPB University2
Irma Herawati SupartoIPB University2
Alina AkhdiyaIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development2
Maria Indah PurnamasariMontana State University2
Yepy H. RustamAfiliasi tidak diketahui2
Maggy Thenawidjaya SuhartonoIPB University1
Dian HendrayantiUniversity of Indonesia1
Dwi Andreas SantosaIPB University1
ALINA AKHADIYAAfiliasi tidak diketahui1
Nurul AiniIPB University1
Walter R. ErdelenIPB University1
Muhammad ZairinIPB University1
Ridwan AffandiIPB University1
Yusminah HalaState University of Makassar1
Sukenda SukendaIPB University1
Budi TjahjonoIPB University1
R. HarlingScottish Agricultural College1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Analysis of Intestinal Mucosal Immunoglobulin A in Sprague Dawley Rats Supplemented with Tempeh</dc:title>
	<dc:creator xml:lang="en">SOKA, SUSAN</dc:creator>
	<dc:creator xml:lang="en">SUWANTO, ANTONIUS</dc:creator>
	<dc:creator xml:lang="en">RUSMANA, IMAN</dc:creator>
	<dc:creator xml:lang="en">SAJUTHI, DONDIN</dc:creator>
	<dc:creator xml:lang="en">ISKANDRIATI, DIAH</dc:creator>
	<dc:creator xml:lang="en">JESSICA, KATHARINA</dc:creator>
	<dc:subject xml:lang="en">Tempeh</dc:subject>
	<dc:subject xml:lang="en">IgA</dc:subject>
	<dc:subject xml:lang="en">RT-PCR</dc:subject>
	<dc:subject xml:lang="en">ELISA</dc:subject>
	<dc:description xml:lang="en">Tempeh is a well-known Indonesian fermented food made from soybean. During the fermentation process, microorganisms play an important role in the flavor, texture, and nutritional quality of tempeh. Tempeh has been show to have immuno-modulatory and immune-stimulating properties that may also be caused by the microorganisms in tempeh as they interact between the microbial population in the intestinal tract. The objective of this study was to quantify IgA gene expression at both the transcription and translation levels in Sprague Dawley (SD) rats supplemented with tempeh. A total of 6 female SD rats were divided into 3 groups of 2 rats. The first group was the control and was fed a standard diet without tempeh. The second- and third group were fed with a standard diet supplemented with raw and cooked tempeh, respectively. Ileum tissue samples were collected after tempeh supplementation for 28 days. RNA was extracted from ileum samples, and measurement of IgA gene expression was further analyzed using semi quantitative real-time PCR. The concentration of IgA protein was quantified from ileum lysate using the half sandwich ELISA method. IgA gene expressions in rats supplemented with raw, and with cooked tempeh, were 1.18 and 1.17 fold higher, respectively, compared to the control group. Moreover, IgA protein secretion levels also increased 2.46 and 2.08 fold, respectively, compared to the control group. The result of this study indicates that both raw and cooked tempeh may stimulate IgA secretion, and also that both viable and non-viable microorganisms might stimulate IgA gene expression.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2015-01-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/9220</dc:identifier>
	<dc:identifier>10.4308/hjb.22.1.48</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 22 No. 1 (2015): January 2015; 48</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/9220/pdf</dc:relation>
</oai_dc:dc>