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Sucrase Activity and Exopolysaccharide Partial Characterization From Three Weissella confusa Strains

HAYATI Journal of Biosciences · Vol. 22 No. 3 (2015)

DOI
10.4308/hjb.22.3.130
ISSN
2086-4094, 1978-3019
Terbit
2015-12-30
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Exopolysaccharides (EPSs) produced by lactic acid bacteria have been well known for their important economic value in food, pharmaceutical and health industries. Large extracellular enzyme sucrases are used by lactic acid bacteria to polymerize EPS, i.e. fructansucrase and glucansucrase. This study aimed to characterize sucrase activity of three Weissella confusa strains MBF8-1, MBF8-2 and MBFCNC-2(1), which were isolated previously from local beverages and their EPS products as well. All strains showed ability to form mucoid and slimy colonies by visual inspection on agar plate using raffinose as substrate suggesting that they possessed fructansucrase activity besides glucansucrase. Obtained EPS products were characterized by HPLC analysis after hydrolysis using 3% TCA at 100C for 1 hour, and by viscosity as well. All strains exhibited similar peak patterns, assuming that all of them possessed fructan EPS product. Supernatant and cell pellet were also analyzed by in situ activity assay performing periodic acid Schiff staining after polyacrylamide gel electrophoresis; only cell pellet showed sucrase activity. Viscosity observation showed that EPS products from all strains were able to increase the viscosity slightly.

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  • University of Indonesia
  • National Research and Innovation Agency
  • IPB University
  • The University of Osaka
  • Scottish Agricultural College
  • Badan Pengkajian dan Penerapan Teknologi
  • Syarif Hidayatullah State Islamic University Jakarta
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Rekan penulisOrganisasiArtikel bersama
Antonius SuwantoIPB University2
Apon Zaenal MustopaNational Research and Innovation Agency2
Bugi Ratno BudiartoNational Research and Innovation Agency2
Is HeliantiNational Research and Innovation Agency2
Fairuz Andini FatiningtyasUniversity of Indonesia2
Riswanto NapitupuluUniversity of Indonesia2
Esti PuspitasariIPB University1
Walter R. ErdelenIPB University1
Budi TjahjonoIPB University1
R. HarlingScottish Agricultural College1
Mohamad SadikinUniversity of Indonesia1
Wahono SumaryonoBadan Pengkajian dan Penerapan Teknologi1
Trismilah MargonoUniversity of Indonesia1
Stella VaniaUniversity of Indonesia1
Nurul AmiliaSyarif Hidayatullah State Islamic University Jakarta1
Tegar AprilianUniversitas Teknologi Sumbawa1
Baso ManguntungiUniversitas Sulawesi Barat1
Ahmad BaikuniUniversity of Indonesia1
Fathan Luthfi HawariUniversity of Indonesia1
SutriyoUniversity of Indonesia1
Delly RamadonUniversity of Indonesia1
Maimunah MaimunahUniversity of Indonesia1
Ratih RinendyaputriKementerian Kesehatan Republik Indonesia1
Emilia Rahmadaniah UtamiUniversity of Indonesia1
Masahiro Kino‐okaThe University of Osaka1
Riku YamamotoThe University of Osaka1
Anis Herliyati MahsunahNational Research and Innovation Agency1
Rikno HarmokoNational Research and Innovation Agency1
Darmawati DarmawatiUniversity of Indonesia1
Siti Irma RahmawatiNational Research and Innovation Agency1
Fauzia Nurul IzzatiNational Research and Innovation Agency1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Sucrase Activity and Exopolysaccharide Partial Characterization From Three Weissella confusa Strains</dc:title>
	<dc:creator xml:lang="en">Malik, Amarila</dc:creator>
	<dc:creator xml:lang="en">Sheilla, Sheilla</dc:creator>
	<dc:creator xml:lang="en">Firdausi, Wangi</dc:creator>
	<dc:creator xml:lang="en">Handayani, Tri</dc:creator>
	<dc:creator xml:lang="en">Saepudin, Endang</dc:creator>
	<dc:subject xml:lang="en">exopolysaccharide</dc:subject>
	<dc:subject xml:lang="en">fructansucrase</dc:subject>
	<dc:subject xml:lang="en">in situ PAS staining</dc:subject>
	<dc:subject xml:lang="en">raffinose</dc:subject>
	<dc:subject xml:lang="en">sucrase</dc:subject>
	<dc:subject xml:lang="en">Weissella confusa</dc:subject>
	<dc:description xml:lang="en">Exopolysaccharides (EPSs) produced by lactic acid bacteria have been well known for their important economic value in food, pharmaceutical and health industries. Large extracellular enzyme sucrases are used by lactic acid bacteria to polymerize EPS, i.e. fructansucrase and glucansucrase. This study aimed to characterize sucrase activity of three Weissella confusa strains MBF8-1, MBF8-2 and MBFCNC-2(1), which were isolated previously from local beverages and their EPS products as well. All strains showed ability to form mucoid and slimy colonies by visual inspection on agar plate using raffinose as substrate suggesting that they possessed fructansucrase activity besides glucansucrase. Obtained EPS products were characterized by HPLC analysis after hydrolysis using 3% TCA at 100C for 1 hour, and by viscosity as well. All strains exhibited similar peak patterns, assuming that all of them possessed fructan EPS product. Supernatant and cell pellet were also analyzed by in situ activity assay performing periodic acid Schiff staining after polyacrylamide gel electrophoresis; only cell pellet showed sucrase activity. Viscosity observation showed that EPS products from all strains were able to increase the viscosity slightly.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2015-12-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/10774</dc:identifier>
	<dc:identifier>10.4308/hjb.22.3.130</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 22 No. 3 (2015): July 2015; 130</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/10774/8320</dc:relation>
</oai_dc:dc>