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Antibacterial, Antifungal and Anticancer Activity of Five Strains of Soil Microorganisms Isolated From Tangkuban Perahu Mountain by Fermentation

HAYATI Journal of Biosciences · Vol. 22 No. 4 (2016)

DOI
10.4308/hjb.22.4.186
ISSN
2086-4094, 1978-3019
Terbit
2016-05-23
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Microorganisms were isolated from soil taken from Tangkuban Perahu mountain. Five strains were investigated in this study, designated TP1, TP2, TP3, TP4, and TP5, respectively. Morphological, biochemical and molecular identifications were conducted for all five strains. These isolates were shown to be closely related to Nocardia sp. YIM 65630 (90%), Streptomyces galbus (99%), Aspergillus unguis (86%), Paecilomyces marquandii (100%) and Nocardia niigatensis (95%), respectively. Production of antibacterial, antifungal and anticancer metabolites was done by fermentation. Screening for bioactivity of five isolates was done by testing the fermentation broth against resistant and pathogenic bacteria, fungi and T47D breast cancer cell line. TP2 strain showed the best bioactivity; the metabolite was purified by extraction with ethyl acetate. Antibacterial, antifungal and anticancer activities from the ethyl acetate extract of TP2 strain were tested by agar diffusion, microdilution and MTT. The extract was shown to be active against methicillin resistant Staphylococcus, methicillin sensitive Staphylococcus aureus, methicillin resistant coagulase negative Staphylococcus, vancomycin resistant Enterococcus, Escherichia coli, Microsforum gypseum with the minimum inhibitory concentration (μg/mL) and diameter of inhibition (mm): 150, 35; 150, 30; 300, 35; 300, 35; 300, 29; 4.7, 36, respectively. The IC50 value of the T47D cell line was 457 μg/mL.

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Rekan penulisOrganisasiArtikel bersama
Yosi Agustina HidayatBandung Institute of Technology1
Lingga IkadityaBandung Institute of Technology1
Zulfan ZazuliBandung Institute of Technology1
Zulfian Muhammad FurqonBandung Institute of Technology1
Puspa Dewi Narrij LotulungAfiliasi tidak diketahui1
Rahmaniar MulyaniAfiliasi tidak diketahui1
Anny SulaswatyAfiliasi tidak diketahui1
Nur QaanitaatiAfiliasi tidak diketahui1
Desak Gede Tirta AndiniAfiliasi tidak diketahui1
Eva NursyifaAfiliasi tidak diketahui1
Sukrasno SukrasnoAfiliasi tidak diketahui1
Gunawan Pamudji WidodoAfiliasi tidak diketahui1
Elin Yulinah SukandarAfiliasi tidak diketahui1
Rahmana Emran KartasasmitaAfiliasi tidak diketahui1
Rina HerowatiAfiliasi tidak diketahui1
Tutus Gusdinar KartawinataAfiliasi tidak diketahui1
Heni RachmawatiAfiliasi tidak diketahui1
Marlina IndriastutiAfiliasi tidak diketahui1
Rika HartatiAfiliasi tidak diketahui1
Amirah AdliaAfiliasi tidak diketahui1
Deasy Diah R.W.Afiliasi tidak diketahui1
Setiawan SetiawanAfiliasi tidak diketahui1
Linda AmaliaAfiliasi tidak diketahui1
Kusnandar AnggadiredjaAfiliasi tidak diketahui1
Lisna Anisa FitrianaAfiliasi tidak diketahui1
Nazhifa UfamyAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Antibacterial, Antifungal and Anticancer Activity of Five Strains of Soil Microorganisms Isolated From Tangkuban Perahu Mountain by Fermentation</dc:title>
	<dc:creator xml:lang="en">Andayani, Desak Gede Sri</dc:creator>
	<dc:creator xml:lang="en">Sukandar, Ukan</dc:creator>
	<dc:creator xml:lang="en">Sukandar, Elin Y.</dc:creator>
	<dc:creator xml:lang="en">Adnyana, I. Ketut</dc:creator>
	<dc:subject xml:lang="en">antibacterial</dc:subject>
	<dc:subject xml:lang="en">antifungal and anticancer activities</dc:subject>
	<dc:subject xml:lang="en">ethyl acetate extraction</dc:subject>
	<dc:subject xml:lang="en">fermentation</dc:subject>
	<dc:subject xml:lang="en">identification</dc:subject>
	<dc:description xml:lang="en">Microorganisms were isolated from soil taken from Tangkuban Perahu mountain. Five strains were investigated in this study, designated TP1, TP2, TP3, TP4, and TP5, respectively. Morphological, biochemical and molecular identifications were conducted for all five strains. These isolates were shown to be closely related to Nocardia sp. YIM 65630 (90%), Streptomyces galbus (99%), Aspergillus unguis (86%), Paecilomyces marquandii (100%) and Nocardia niigatensis (95%), respectively. Production of antibacterial, antifungal and anticancer metabolites was done by fermentation. Screening for bioactivity of five isolates was done by testing the fermentation broth against resistant and pathogenic bacteria, fungi and T47D breast cancer cell line. TP2 strain showed the best bioactivity; the metabolite was purified by extraction with ethyl acetate. Antibacterial, antifungal and anticancer activities from the ethyl acetate extract of TP2 strain were tested by agar diffusion, microdilution and MTT. The extract was shown to be active against methicillin resistant Staphylococcus, methicillin sensitive Staphylococcus aureus, methicillin resistant coagulase negative Staphylococcus, vancomycin resistant Enterococcus, Escherichia coli, Microsforum gypseum with the minimum inhibitory concentration (μg/mL) and diameter of inhibition (mm): 150, 35; 150, 30; 300, 35; 300, 35; 300, 29; 4.7, 36, respectively. The IC50 value of the T47D cell line was 457 μg/mL.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2016-05-23</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/12104</dc:identifier>
	<dc:identifier>10.4308/hjb.22.4.186</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 22 No. 4 (2015): October 2015; 186</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/12104/9325</dc:relation>
</oai_dc:dc>