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Construction of RNA Interference Vector to Silence Aluminum Tolerance Gene Candidate in Rice cv Hawara Bunar

HAYATI Journal of Biosciences · Vol. 23 No. 2 (2016)

DOI
10.4308/hjb.23.2.79
ISSN
2086-4094, 1978-3019
Terbit
2016-11-10
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

One of the aluminum (Al) tolerance gene candidates, namely B11 gene, has been successfully isolated from Al-tolerant rice cv Hawara Bunar. However, the role of the gene in Al tolerance in rice has not been known. RNA interference (RNAi) technique is an effective tool to examine the biological function of the target gene in plant. The objective of the research was to construct RNAi recombinant vector carrying untranslated region of the B11 gene. RNAi recombinant vector carrying 195 bp sized 3′UTR_B11 fragment as a double-stranded RNA (dsRNA) trigger has been successfully constructed using GATEWAY™ cloning technology, pENTR™/D-TOPO® as a shuttle vector, and pANDA vector as a destination vector. RNAi construct was successfully introduced into Agrobacterium tumefaciens AgL0, and has been infected to rice cv Hawara Bunar. Analysis of putative transgenic rice showed eight of 20 plants were transgenic carrying the B11-RNAi construct.

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  • National Research and Innovation Agency
  • University of Darussalam Gontor
  • Institut Agama Islam Negeri Kendari
  • Universitas Rokania
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Suharsono SuharsonoAfiliasi tidak diketahui6
ROHANI CINTA BADIA GINTINGAfiliasi tidak diketahui3
Ence Darmo Jaya SupenaAfiliasi tidak diketahui2
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LATIFAH KOSIM DARUSMANAfiliasi tidak diketahui2
SIHEGIKO KANAYAAfiliasi tidak diketahui2
M. MiftahudinAfiliasi tidak diketahui2
Triadiati TriadiatiAfiliasi tidak diketahui2
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Adi Nur CahyonoAfiliasi tidak diketahui1
Himmah RustiamiNational Research and Innovation Agency1
Tatik ChikmawatiAfiliasi tidak diketahui1
I Putu Gede P. DamayantoAfiliasi tidak diketahui1
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AlimuddinAfiliasi tidak diketahui1
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Agus Oman SudrajatAfiliasi tidak diketahui1
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Ratu Siti AliahAfiliasi tidak diketahui1
Niken TrisnaningrumUniversity of Darussalam Gontor1
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Suryo WiyonoAfiliasi tidak diketahui1
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Emma SuryatiAfiliasi tidak diketahui1
Erina SulistianiAfiliasi tidak diketahui1
Antonius SuwantoAfiliasi tidak diketahui1
Nurlaela NurlaelaInstitut Agama Islam Negeri Kendari1
Juliarni JuliarniUniversitas Rokania1
SuharsonoNational Research and Innovation Agency1
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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Construction of RNA Interference Vector to Silence Aluminum Tolerance Gene Candidate in Rice cv Hawara Bunar</dc:title>
	<dc:creator xml:lang="en">Wahyuningtyas, Windarti</dc:creator>
	<dc:creator xml:lang="en">Miftahudin, Miftahudin</dc:creator>
	<dc:creator xml:lang="en">Widyastuti, Utut</dc:creator>
	<dc:creator xml:lang="en">Tjahjoleksono, Aris</dc:creator>
	<dc:subject xml:lang="en">3′UTR_B11</dc:subject>
	<dc:subject xml:lang="en">Al tolerant</dc:subject>
	<dc:subject xml:lang="en">rice</dc:subject>
	<dc:subject xml:lang="en">RNAi vector</dc:subject>
	<dc:description xml:lang="en">One of the aluminum (Al) tolerance gene candidates, namely B11 gene, has been successfully isolated from Al-tolerant rice cv Hawara Bunar. However, the role of the gene in Al tolerance in rice has not been known. RNA interference (RNAi) technique is an effective tool to examine the biological function of the target gene in plant. The objective of the research was to construct RNAi recombinant vector carrying untranslated region of the B11 gene. RNAi recombinant vector carrying 195 bp sized 3′UTR_B11 fragment as a double-stranded RNA (dsRNA) trigger has been successfully constructed using GATEWAY™ cloning technology, pENTR™/D-TOPO® as a shuttle vector, and pANDA vector as a destination vector. RNAi construct was successfully introduced into Agrobacterium tumefaciens AgL0, and has been infected to rice cv Hawara Bunar. Analysis of putative transgenic rice showed eight of 20 plants were transgenic carrying the B11-RNAi construct.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2016-11-10</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/13940</dc:identifier>
	<dc:identifier>10.4308/hjb.23.2.79</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 23 No. 2 (2016): April 2016; 79</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/13940/10451</dc:relation>
</oai_dc:dc>