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Production of Xylanase by Recombinant Bacillus subtilis DB104 Cultivated in Agroindustrial Waste Medium

HAYATI Journal of Biosciences · Vol. 23 No. 3 (2017)

DOI
10.4308/hjb.23.3.125
ISSN
2086-4094, 1978-3019
Terbit
2017-04-14
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

A recombinant Bacillus subtilis DB104 strain harbouring recombinant plasmid pSKE194 containing an Open Reading Frame (ORF) of endoxylanase and its indigenous promoter from the wild-type B. subtilis AQ1 strain was constructed. This recombinant B. subtilisDB104 strain had higher endoxylanase activity than the nonrecombinant B. subtilisDB104 strain in standard media, such as Luria Bertani (LB) and LB with xylan. The agroindustrial wastes corncobs and tofu liquid waste were chosen as cost-effective carbon and nitrogen sources, respectively, to test the economics of xylanase production using the recombinant B. subtilis DB104 at a larger scale. Submerged fermentation using a 4.5 L working volume fermentor with tofu liquid waste and 4% corncobs produced maximum xylanase activity of 1296 ± 1.2 U/mg (601.7 ± 0.6 U/mL) after 48-hour fermentation at 37°C with 150 rpm agitation; this is more than twofold higher than the activity produced in an Erlenmeyer flask. This is the first report of high xylanase activity produced from recombinant B. subtilis using inexpensive medium. During fermentation, the xylanase degrades corncobs into xylooligosaccharides, showing its potential as an enzyme feed additive or in xylooligosaccharide production.

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  • National Research and Innovation Agency
  • University of Brawijaya
  • Badan Pengkajian dan Penerapan Teknologi
  • University of Indonesia
  • National Center for Genetic Engineering and Biotechnology
  • Universitas PGRI Semarang
  • IPB University
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Rekan penulisOrganisasiArtikel bersama
Sri Rezeki WulandariNational Research and Innovation Agency6
Gabriela Christy SabbathiniNational Research and Innovation Agency4
Maria UlfahNational Research and Innovation Agency3
Maria UlfahNational Research and Innovation Agency3
AbinawantoAfiliasi tidak diketahui2
Lina MulyawatiBadan Pengkajian dan Penerapan Teknologi2
Amarila MalikUniversity of Indonesia2
Fairuz Andini FatiningtyasUniversity of Indonesia2
Riswanto NapitupuluUniversity of Indonesia2
Khomaini HasanUniversitas Jenderal Achmad Yani2
I Gede Eka Perdana PutraNational Research and Innovation Agency2
Tri Yudani Mardining RarasUniversity of Brawijaya2
Mauludy Jutta AjrullahUniversity of Brawijaya2
Ade IhtiarUniversitas PGRI Semarang1
Fibria KaswinarniUniversitas PGRI Semarang1
Valerry Athalia PriyankaAfiliasi tidak diketahui1
Maria UlfahIPB University1
Ajeng Astrini BrahmantiUniversity of Brawijaya1
Erryana MartatiUniversity of Brawijaya1
Mochamad NurcholisUniversity of Brawijaya1
Muthia Rahmah DiniUniversity of Brawijaya1
Kartika Sari CendanaUniversity of Indonesia1
Dini AchnafaniNational Research and Innovation Agency1
Sunni Sofiah AniqahNational Research and Innovation Agency1
Niknik NurhayatiNational Research and Innovation Agency1
Duriya CantasinghAfiliasi tidak diketahui1
Haniyya HaniyyaBadan Pengkajian dan Penerapan Teknologi1
Phitsanu PinmaneeNational Center for Genetic Engineering and Biotechnology1
Kanokarn KocharinNational Center for Genetic Engineering and Biotechnology1
Thidarat NimchuaNational Center for Genetic Engineering and Biotechnology1
Syamsika TahirUniversity of Brawijaya1
Widya Dwi Rukmi PutriUniversity of Brawijaya1
Rofiq SunaryantoBadan Pengkajian dan Penerapan Teknologi1
Maimunah MaimunahUniversity of Indonesia1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Production of Xylanase by Recombinant Bacillus subtilis DB104 Cultivated in Agroindustrial Waste Medium</dc:title>
	<dc:creator xml:lang="en">Helianti, Is</dc:creator>
	<dc:creator xml:lang="en">Ulfah, Maria</dc:creator>
	<dc:creator xml:lang="en">Nurhayati, Niknik</dc:creator>
	<dc:creator xml:lang="en">Suhendar, Dadang</dc:creator>
	<dc:creator xml:lang="en">Finalissari, Anita Kusuma</dc:creator>
	<dc:creator xml:lang="en">Wardani, Agustin Krisna</dc:creator>
	<dc:subject xml:lang="en">corncobs</dc:subject>
	<dc:subject xml:lang="en">cost-effective medium</dc:subject>
	<dc:subject xml:lang="en">endoxylanase</dc:subject>
	<dc:subject xml:lang="en">recombinant Bacillus subtilis DB104</dc:subject>
	<dc:subject xml:lang="en">tofu liquid waste</dc:subject>
	<dc:subject xml:lang="en">xylooligosaccharides</dc:subject>
	<dc:description xml:lang="en">A recombinant Bacillus subtilis DB104 strain harbouring recombinant plasmid pSKE194 containing an Open Reading Frame (ORF) of endoxylanase and its indigenous promoter from the wild-type B. subtilis AQ1 strain was constructed. This recombinant B. subtilisDB104 strain had higher endoxylanase activity than the nonrecombinant B. subtilisDB104 strain in standard media, such as Luria Bertani (LB) and LB with xylan. The agroindustrial wastes corncobs and tofu liquid waste were chosen as cost-effective carbon and nitrogen sources, respectively, to test the economics of xylanase production using the recombinant B. subtilis DB104 at a larger scale. Submerged fermentation using a 4.5 L working volume fermentor with tofu liquid waste and 4% corncobs produced maximum xylanase activity of 1296 ± 1.2 U/mg (601.7 ± 0.6 U/mL) after 48-hour fermentation at 37°C with 150 rpm agitation; this is more than twofold higher than the activity produced in an Erlenmeyer flask. This is the first report of high xylanase activity produced from recombinant B. subtilis using inexpensive medium. During fermentation, the xylanase degrades corncobs into xylooligosaccharides, showing its potential as an enzyme feed additive or in xylooligosaccharide production.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2017-04-14</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/16105</dc:identifier>
	<dc:identifier>10.4308/hjb.23.3.125</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 23 No. 3 (2016): July 2016; 125</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/16105/11899</dc:relation>
</oai_dc:dc>