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The Influence of Thidiazuron on Direct Somatic Embryo Formation from Various Types of Explant in Phalaenopsis amabilis (L.) Blume Orchid

HAYATI Journal of Biosciences · Vol. 24 No. 4 (2018)

DOI
10.4308/hjb.24.4.201
ISSN
2086-4094, 1978-3019
Terbit
2018-03-27
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Phalaenopsis amabilis is an important national flower of Indonesia as a parent for orchidbreeding, so that needs a good strategy to produce high number of plants. The objective of this research is to analyze the use of thidiazuron (TDZ) for producing high number of plantlets, through directly induction of somatic embryos (SEs) from various explants. The method was used 20 each of protocorms, leaves, stems and roots as explants. The explants were dissected transversely, then put on various culture media: New Phalaenopsis (NP) and NP + (1, 2, 3) mgL−1 TDZ. Cultures were maintained at 25°C with continous white light. The formation of SEs was observed every week for 8 weeks. The results showed that SEs formation increased inline with the addition of TDZ concentration to the NP medium, for both velocity and amount of SEs formation. In NP0, SEs were formed at (26.07 ± 0.73) days after inoculation of protocorm, whereas on NP + (1, 2, and 3 mgL−1) TDZ, SEs were formed at (17.85 ± 0.67) days, (15 ± 0.64) days, and (11 ± 0.64) days, respectively. All types of explants formed SEs on NP + TDZ (1–3 mgL−1), whereas only 14 of 20 protocorms produced SEs (70%), and 8 of 20 stems formed SEs (40%) in NP0. In roots, SEs was formed on NP + 2 mgL−1 TDZ and NP + 3 mgL−1 TDZ. For stems, the highest amount of SEs (28.25 ± 1.07) was reached on NP + 3 mgL−1 TDZ, followed by protocorm (23.30 ± 1.13) SEs and roots (8.25 ± 0.68) SEs. In contrast, in NP0, the amount of SEs was very low (1.25 ± 0.46) from stem and (1.50 ± 0.65) from protocorms, there was no evidence of SEs formation in the leaves and roots.

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Sukarti MoeljopawiroAfiliasi tidak diketahui11
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Agus Budi SetiawanAfiliasi tidak diketahui5
Jose Gutierrez-MarcosAfiliasi tidak diketahui5
Aries Bagus SasongkoUniversitas Gadjah Mada5
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Naufal Ghozi Aditya PerdanaAfiliasi tidak diketahui4
Febri Yuda KurniawanAfiliasi tidak diketahui3
Jaka WidadaUniversitas Gadjah Mada3
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Endang SulistyaningsihAfiliasi tidak diketahui2
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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">The Influence of Thidiazuron on Direct Somatic Embryo Formation from Various Types of Explant in Phalaenopsis amabilis (L.) Blume Orchid</dc:title>
	<dc:creator xml:lang="en">Mose, Windi</dc:creator>
	<dc:creator xml:lang="en">Indrianto, Ari</dc:creator>
	<dc:creator xml:lang="en">Purwantoro, Aziz</dc:creator>
	<dc:creator xml:lang="en">Semiarti, Endang</dc:creator>
	<dc:subject xml:lang="en">New Phalaenopsis medium</dc:subject>
	<dc:subject xml:lang="en">Phalaenopsis amabilis orchid</dc:subject>
	<dc:subject xml:lang="en">somatic embryo</dc:subject>
	<dc:subject xml:lang="en">thidiazuron</dc:subject>
	<dc:subject xml:lang="en">various explants</dc:subject>
	<dc:description xml:lang="en">Phalaenopsis amabilis is an important national flower of Indonesia as a parent for orchidbreeding, so that needs a good strategy to produce high number of plants. The objective of this research is to analyze the use of thidiazuron (TDZ) for producing high number of plantlets, through directly induction of somatic embryos (SEs) from various explants. The method was used 20 each of protocorms, leaves, stems and roots as explants. The explants were dissected transversely, then put on various culture media: New Phalaenopsis (NP) and NP + (1, 2, 3) mgL−1 TDZ. Cultures were maintained at 25°C with continous white light. The formation of SEs was observed every week for 8 weeks. The results showed that SEs formation increased inline with the addition of TDZ concentration to the NP medium, for both velocity and amount of SEs formation. In NP0, SEs were formed at (26.07 ± 0.73) days after inoculation of protocorm, whereas on NP + (1, 2, and 3 mgL−1) TDZ, SEs were formed at (17.85 ± 0.67) days, (15 ± 0.64) days, and (11 ± 0.64) days, respectively. All types of explants formed SEs on NP + TDZ (1–3 mgL−1), whereas only 14 of 20 protocorms produced SEs (70%), and 8 of 20 stems formed SEs (40%) in NP0. In roots, SEs was formed on NP + 2 mgL−1 TDZ and NP + 3 mgL−1 TDZ. For stems, the highest amount of SEs (28.25 ± 1.07) was reached on NP + 3 mgL−1 TDZ, followed by protocorm (23.30 ± 1.13) SEs and roots (8.25 ± 0.68) SEs. In contrast, in NP0, the amount of SEs was very low (1.25 ± 0.46) from stem and (1.50 ± 0.65) from protocorms, there was no evidence of SEs formation in the leaves and roots.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2018-03-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/20829</dc:identifier>
	<dc:identifier>10.4308/hjb.24.4.201</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 24 No. 4 (2017): October 2017; 201</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/20829/14270</dc:relation>
</oai_dc:dc>