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Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology

HAYATI Journal of Biosciences · Vol. 25 No. 3 (2018)

DOI
10.4308/hjb.25.3.115
ISSN
2086-4094, 1978-3019
Terbit
2018-10-24
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Cellulase is a very important enzyme for lignocelluloses based ethanol production. Bagasse contains mainly cellulose (57.76%), hemicellulose (12.44%), lignin (21.34%), and others (7.96%). Lignocellulosic material has been considered as the good option for cellulase production because it is cheap and already available in a huge amount. The objective of this research was to produce cellulase enzyme and to optimize it by using response surface methodology. The bagasse with water content of 80% was incubated with 2 ml inoculum of Aspergillus niger ITBCC L74 in a 250 ml Erlenmeyer flask. After reaching the specified time the enzyme was extracted and then determined for its activity. Effect of process parameters such as pH, urea and MgCl2 addition were studied. The optimal cellulase activity was achieved at urea concentration of 4.5% (w/w), MgCl2 concentration of 1 mM and pH of 3.5, with maximum enzyme activity was 0.630 U/gr.

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  • Diponegoro University
  • Institut Teknologi Indonesia
  • State University of Semarang
  • Muhammadiyah University of Surakarta
  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • University of Shahrood
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Rekan penulisOrganisasiArtikel bersama
Widayat WidayatDiponegoro University5
Kusmiyati KusmiyatiMuhammadiyah University of Surakarta5
Dessy AriyantiDiponegoro University4
Linda Aliffia YoshiInstitut Teknologi Indonesia3
Arief Taufik BudimanUniversitas Gadjah Mada3
Athanasia Amanda SeptevaniNational Research and Innovation Agency3
Enjarlis EnjarlisInstitut Teknologi Indonesia2
Muhamad Maulana Azimatun NurDiponegoro University2
Apsari Puspita AiniDiponegoro University2
Mohammad Hossein AhmadiUniversity of Shahrood2
Prima Astuti HandayaniState University of Semarang2
Andin Vita AmaliaState University of Semarang1
Tri Retnaningsih SoeprobowatiDiponegoro University1
Rita Dwi RatnaniUniversitas Wahid Hasyim1
W. WidiyantoDiponegoro University1
M. A. AdhiState University of Semarang1
Masthura MasthuraUniversitas Islam Negeri Sumatera Utara1
J. JoelianingsihInstitut Teknologi Indonesia1
Ratna Dewi KusumaningtyasState University of Semarang1
Imam Novrizal AjiState University of Semarang1
R RatnawatiHasanuddin University1
S. SlametUniversity of Indonesia1
Yuli Amalia HusnilInstitut Teknologi Indonesia1
Sri Puji LestariDiponegoro University1
Indro SumantriDiponegoro University1
Sakina Tunissa AnarkiMuhammadiyah University of Surakarta1
Sabda Wahyu NugrohoMuhammadiyah University of Surakarta1
Reistu WidiastutikMuhammadiyah University of Surakarta1
Heri SutantoDiponegoro University1
Fatkhiyatus Sa’adahDiponegoro University1
Ilham AlkianDiponegoro University1
Sri Rezki Mardiah SyamDiponegoro University1
B. BudiyonoDiponegoro University1
Ganang Dwi HartantoDiponegoro University1
Adrianus Kristyo PrabowoDiponegoro University1
Agnes Priska TiarasukmaDiponegoro University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology</dc:title>
	<dc:creator xml:lang="en">Abdullah, Abdullah</dc:creator>
	<dc:creator xml:lang="en">Hamid, Hamid</dc:creator>
	<dc:creator xml:lang="en">Christwardana, Marcelinus</dc:creator>
	<dc:creator xml:lang="en">Hadiyanto, H.</dc:creator>
	<dc:subject xml:lang="en">Aspergillus niger</dc:subject>
	<dc:subject xml:lang="en">bagasse</dc:subject>
	<dc:subject xml:lang="en">cellulase</dc:subject>
	<dc:subject xml:lang="en">optimization</dc:subject>
	<dc:subject xml:lang="en">solid state fermentation</dc:subject>
	<dc:description xml:lang="en">Cellulase is a very important enzyme for lignocelluloses based ethanol production. Bagasse contains mainly cellulose (57.76%), hemicellulose (12.44%), lignin (21.34%), and others (7.96%). Lignocellulosic material has been considered as the good option for cellulase production because it is cheap and already available in a huge amount. The objective of this research was to produce cellulase enzyme and to optimize it by using response surface methodology. The bagasse with water content of 80% was incubated with 2 ml inoculum of Aspergillus niger ITBCC L74 in a 250 ml Erlenmeyer flask. After reaching the specified time the enzyme was extracted and then determined for its activity. Effect of process parameters such as pH, urea and MgCl2 addition were studied. The optimal cellulase activity was achieved at urea concentration of 4.5% (w/w), MgCl2 concentration of 1 mM and pH of 3.5, with maximum enzyme activity was 0.630 U/gr.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2018-10-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/23824</dc:identifier>
	<dc:identifier>10.4308/hjb.25.3.115</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 25 No. 3 (2018): July 2018; 115</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/23824/15500</dc:relation>
</oai_dc:dc>