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Isolation and Characterization of C-C Chemokine Ligand 7 (CCL7) in Cynomolgus Macaques

HAYATI Journal of Biosciences · Vol. 26 No. 3 (2019)

DOI
10.4308/hjb.26.3.129
ISSN
2086-4094, 1978-3019
Terbit
2019-12-02
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Cynomolgus macaques (Macaca fascicularis) are an established animal model of asthma, which exhibit different responses to allergen exposure that are clinically relevant. The chemokine ligand gene (CCL7) encodes Monocyte Chemotactic Protein-3, which has an important role in asthma pathogenesis. While CCL7 polymorphism in humans is associated with asthma phenotype, very little is known about CCL7 in nonhuman primate models of respiratory disease. The objective of this study was to isolate and characterize CCL7 gene in cynomolgus macaques of Indonesian origin. In this study, we used sequencing and bioinformatics technique for gene isolation, characterization, and protein 3D structure prediction. We isolated a 2253 base-pair (bp) sequence of CCL7 in cynomolgus macaques, which exhibited 95% similarity in coding sequence to human CCL7. The amino acid sequence was more closely clustered with human CCL7 than with that of rodents. Importantly, the predictive protein structure of CCL7 was similar to that in humans. These similarities in CCL7 suggests the potential of cynomolgus macaque as a translational model to study asthma, particularly in the context of genetics and role of chemokines such as CCL7.

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  • IPB University
  • University of Indonesia
  • Atma Jaya Catholic University of Indonesia
  • Porsolt (France)
  • Universitas Katolik Widya Mandala Surabaya
  • National Research and Innovation Agency
  • University of Zurich
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Rekan penulisOrganisasiArtikel bersama
Irma Herawati SupartoIPB University5
Bambang PurwantaraIPB University3
Huda Shalahudin DarusmanIPB University3
Antonius SuwantoIPB University2
İman RusmanaIPB University2
Susan SokaAtma Jaya Catholic University of Indonesia2
KATHARINA JESSICAAtma Jaya Catholic University of Indonesia2
Dyah Perwitasari‐FarajallahIPB University2
I Wayan Teguh WibawanIPB University2
Fitry TafziAfiliasi tidak diketahui1
Nuri AndarwulanIPB University1
Puspo Edi GiriwonoIPB University1
Dewi Apri AstutiIPB University1
I Nengah BudiarsaIPB University1
Hera MaheshwariAfiliasi tidak diketahui1
Hadi Sukardi AlikodraAfiliasi tidak diketahui1
Reviany WidjajakusumaIPB University1
PUDJI ASTUTIAfiliasi tidak diketahui1
Luthfiralda SjahfirdiUniversity of Indonesia1
Paini Sri WidyawatiUniversitas Katolik Widya Mandala Surabaya1
Christofora Hanny WijayaIPB University1
Peni Suprapti HardjosworoIPB University1
Puji RiantiIPB University1
Joko PamungkasNational Research and Innovation Agency1
Alexander NaterUniversity of Zurich1
Michael KrützenIPB University1
Arief BoedionoIPB University1
Agus HarsoyoIPB University1
Yoga YuniadiUniversity of Indonesia1
Silmi MariyaIPB University1
Rizki Aulia AnsariIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Isolation and Characterization of C-C Chemokine Ligand 7 (CCL7) in Cynomolgus Macaques</dc:title>
	<dc:creator xml:lang="en">Mariya, Sela S.</dc:creator>
	<dc:creator xml:lang="en">Dewi, Fitriya N.</dc:creator>
	<dc:creator xml:lang="en">Villiandra, Villiandra</dc:creator>
	<dc:creator xml:lang="en">Paramastri, Yasmina A.</dc:creator>
	<dc:creator xml:lang="en">Iskandriati, Diah</dc:creator>
	<dc:creator xml:lang="en">Saepuloh, Uus</dc:creator>
	<dc:creator xml:lang="en">Hayes, Eric</dc:creator>
	<dc:creator xml:lang="en">Pamungkas, Joko</dc:creator>
	<dc:creator xml:lang="en">Sajuthi, Dondin</dc:creator>
	<dc:subject xml:lang="en">Asthma</dc:subject>
	<dc:subject xml:lang="en">CCL7</dc:subject>
	<dc:subject xml:lang="en">gene characterization</dc:subject>
	<dc:subject xml:lang="en">MCP3</dc:subject>
	<dc:subject xml:lang="en">nonhuman primates</dc:subject>
	<dc:description xml:lang="en">Cynomolgus macaques (Macaca fascicularis) are an established animal model of asthma, which exhibit different responses to allergen exposure that are clinically relevant. The chemokine ligand gene (CCL7) encodes Monocyte Chemotactic Protein-3, which has an important role in asthma pathogenesis. While CCL7 polymorphism in humans is associated with asthma phenotype, very little is known about CCL7 in nonhuman primate models of respiratory disease. The objective of this study was to isolate and characterize CCL7 gene in cynomolgus macaques of Indonesian origin. In this study, we used sequencing and bioinformatics technique for gene isolation, characterization, and protein 3D structure prediction. We isolated a 2253 base-pair (bp) sequence of CCL7 in cynomolgus macaques, which exhibited 95% similarity in coding sequence to human CCL7. The amino acid sequence was more closely clustered with human CCL7 than with that of rodents. Importantly, the predictive protein structure of CCL7 was similar to that in humans. These similarities in CCL7 suggests the potential of cynomolgus macaque as a translational model to study asthma, particularly in the context of genetics and role of chemokines such as CCL7.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2019-12-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/28504</dc:identifier>
	<dc:identifier>10.4308/hjb.26.3.129</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 26 No. 3 (2019): July 2019; 129</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/28504/18086</dc:relation>
</oai_dc:dc>