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Pre-treatment and Suitable Reagent Enabled a Reliable and Consistent for Molecular Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc4)

HAYATI Journal of Biosciences · Vol. 26 No. 4 (2019)

DOI
10.4308/hjb.26.4.196
ISSN
2086-4094, 1978-3019
Terbit
2019-12-28
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Fusarium wilt which is caused by the soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc), is one of important diseases in banana plant. Foc tropical race 4 (Foc4) is the most pathogenic race which could infect various banana cultivars including Cavendish cultivar which was previously considered as resistant cultivar. Molecular detection of Foc using PCR analysis is indispensable to determine the race of Foc. We demonstrate a faster DNA isolation procedure described in previous method by substituting sodium acetate precipitation with ammonium acetate precipitation without affecting the result. Based on our experience, some fungal genomes were troublesome to be amplified. We suggested pre-treatment step prior to amplification procedure by incubating fungal DNA in 65°C for 10 minutes for any samples of fungal genome, including stubborn samples, before mixing into PCR mix reagent. PCR reagents should be tested for stubborn samples since some of the reagents were unable to amplify the desired DNA fragment. Pre-treatment and the choice of robust PCR reagent should be taken into consideration for a reliable and consistent Foc4 molecular detection result.

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  • Bandung Institute of Technology
  • Sultan Ageng Tirtayasa University
  • Udayana University
  • The University of Osaka
  • Atma Jaya Catholic University of Indonesia
  • Andalas University
  • Tokyo University of Agriculture and Technology
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Rekan penulisOrganisasiArtikel bersama
Karlia MeithaBandung Institute of Technology6
Tjandra AnggraeniBandung Institute of Technology4
Sony SuhandonoBandung Institute of Technology4
Agus SutantoAndalas University3
Ketut WikantikaBandung Institute of Technology3
Husna NugrahaprajaBandung Institute of Technology3
Aksarani ‘Sa PratiwiBandung Institute of Technology3
Indah Juwita SariSultan Ageng Tirtayasa University2
Ahmad FaizalBandung Institute of Technology2
Rizkita Rahmi EsyantiBandung Institute of Technology2
I NyomanUdayana University2
Veronica Sri Harjati SuhardiBandung Institute of Technology2
Sukrasno SukrasnoBandung Institute of Technology2
Muhandinni ZahraBandung Institute of Technology2
Indria WahyuniSultan Ageng Tirtayasa University1
Rida Oktorida KhastiniSultan Ageng Tirtayasa University1
Ewi AwaliyatiSultan Ageng Tirtayasa University1
Andriana SusilowatiSultan Ageng Tirtayasa University1
Enggar UtariSultan Ageng Tirtayasa University1
Adi PancoroBandung Institute of Technology1
Panca Jarot SantosoAfiliasi tidak diketahui1
Raihanah Yurizka KinantiBandung Institute of Technology1
Yooce YustianaBandung Institute of Technology1
Anca Awal SembadaBandung Institute of Technology1
Yuli Setyo IndartonoBandung Institute of Technology1
I. Wuled LenggoroTokyo University of Agriculture and Technology1
Pijar Riza AnugerahBandung Institute of Technology1
Herafi ZaskiaBandung Institute of Technology1
Annisa AmaliaBandung Institute of Technology1
Sastia Prama PutriThe University of Osaka1
Kana YamamotoThe University of Osaka1
Eiichiro FukusakiThe University of Osaka1
I Made SukewijayaUdayana University1
I Nyoman Gede UstriyanaUdayana University1
Agung Budi HartoBandung Institute of Technology1
Prila Ayu Dwi PrastiwiBandung Institute of Technology1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Pre-treatment and Suitable Reagent Enabled a Reliable and Consistent for Molecular Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc4)</dc:title>
	<dc:creator xml:lang="en">Karmawan, Listya Utami</dc:creator>
	<dc:creator xml:lang="en">Dwivany, Fenny Martha</dc:creator>
	<dc:creator xml:lang="en">Esyanti, Rizkita Rachmi</dc:creator>
	<dc:creator xml:lang="en">Aryantha, I Nyoman Pugeg</dc:creator>
	<dc:subject xml:lang="en">DNA isolation</dc:subject>
	<dc:subject xml:lang="en">genome pre-treatment</dc:subject>
	<dc:subject xml:lang="en">Foc detection method</dc:subject>
	<dc:subject xml:lang="en">banana</dc:subject>
	<dc:subject xml:lang="en">Fusarium wilt</dc:subject>
	<dc:subject xml:lang="en">food and agriculture</dc:subject>
	<dc:description xml:lang="en">Fusarium wilt which is caused by the soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc), is one of important diseases in banana plant. Foc tropical race 4 (Foc4) is the most pathogenic race which could infect various banana cultivars including Cavendish cultivar which was previously considered as resistant cultivar. Molecular detection of Foc using PCR analysis is indispensable to determine the race of Foc. We demonstrate a faster DNA isolation procedure described in previous method by substituting sodium acetate precipitation with ammonium acetate precipitation without affecting the result. Based on our experience, some fungal genomes were troublesome to be amplified. We suggested pre-treatment step prior to amplification procedure by incubating fungal DNA in 65°C for 10 minutes for any samples of fungal genome, including stubborn samples, before mixing into PCR mix reagent. PCR reagents should be tested for stubborn samples since some of the reagents were unable to amplify the desired DNA fragment. Pre-treatment and the choice of robust PCR reagent should be taken into consideration for a reliable and consistent Foc4 molecular detection result.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2019-12-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/28885</dc:identifier>
	<dc:identifier>10.4308/hjb.26.4.196</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 26 No. 4 (2019): October 2019; 196</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/28885/18227</dc:relation>
</oai_dc:dc>