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Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium

HAYATI Journal of Biosciences · Vol. 27 No. 2 (2020)

DOI
10.4308/hjb.27.2.89
ISSN
2086-4094, 1978-3019
Terbit
2020-04-01
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.

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Ni Wayan Kurniani KarjaIPB University4
Harry MurtiAfiliasi tidak diketahui4
Muhammad RizalUniversitas Halu Oleo3
HERDISAfiliasi tidak diketahui3
Mohamad Agus SetiadiAfiliasi tidak diketahui3
Srihadi AgungpriyonoAfiliasi tidak diketahui3
RIKA RAFFIUDINAfiliasi tidak diketahui3
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Indra BachtiarAfiliasi tidak diketahui3
I Ketut Mudite AdnyaneAfiliasi tidak diketahui3
Adi WinartoAfiliasi tidak diketahui2
Sri Anna MarliyatiAfiliasi tidak diketahui2
ITA DJUWITAAfiliasi tidak diketahui2
KAZUHIRO KIKUCHIAfiliasi tidak diketahui2
Takdir SailiAfiliasi tidak diketahui2
. YulnawatiAfiliasi tidak diketahui2
Fahri FahriUniversitas Negeri Surabaya2
Tri AtmowidiAfiliasi tidak diketahui2
Nisfia Rakhmatun NisaAfiliasi tidak diketahui2
Wahono Esthi PrasetyaningtyasIPB University2
Aryani Sismin SatyaningtijasAfiliasi tidak diketahui1
Resti Rahma DiantiAfiliasi tidak diketahui1
Alif Iman FitriantoAfiliasi tidak diketahui1
Adkhilni UtamiAfiliasi tidak diketahui1
Wining AstiniAfiliasi tidak diketahui1
Adisti DwijayantiAfiliasi tidak diketahui1
Frans Dhyanagiri SuyatnaAfiliasi tidak diketahui1
Rahayu Dewi SoeyonoAfiliasi tidak diketahui1
Rita IsmawatiAfiliasi tidak diketahui1
Hardinsyah HardinsyahAfiliasi tidak diketahui1
Choirul Anna Nur AfifahAfiliasi tidak diketahui1
Yuni NurwatiAfiliasi tidak diketahui1
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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium</dc:title>
	<dc:creator xml:lang="en">Budiariati, Vista </dc:creator>
	<dc:creator xml:lang="en">Budiono, Dwi </dc:creator>
	<dc:creator xml:lang="en">Fahrudin, Mokhamad </dc:creator>
	<dc:creator xml:lang="en">Juliandi, Berry</dc:creator>
	<dc:creator xml:lang="en">Rinendyaputri, Ratih </dc:creator>
	<dc:creator xml:lang="en">Boediono, Arief </dc:creator>
	<dc:description xml:lang="en">Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2020-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/31493</dc:identifier>
	<dc:identifier>10.4308/hjb.27.2.89</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 27 No. 2 (2020): April 2020; 89</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/31493/20069</dc:relation>
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