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Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis

HAYATI Journal of Biosciences · Vol. 28 No. 2 (2021)

DOI
10.4308/hjb.28.2.136
ISSN
2086-4094, 1978-3019
Terbit
2021-04-01
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.

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  • Universitas Gadjah Mada
  • State University of Malang
  • National Research and Innovation Agency
  • Universitas Airlangga
  • IPB University
  • University of Edinburgh
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Rekan penulisOrganisasiArtikel bersama
Tia ErfiantiUniversitas Gadjah Mada8
Arief Taufik BudimanUniversitas Gadjah Mada6
Renata Adaranyssa Egistha PutriUniversitas Gadjah Mada4
Brilian Ryan SadewoUniversitas Gadjah Mada4
Dedy KurniantoNational Research and Innovation Agency3
Khusnul Qonita MaghfirohUniversitas Gadjah Mada3
Ria AmeliaUniversitas Gadjah Mada3
Dedy KurniantoUniversitas Gadjah Mada3
Budi Setiadi DaryonoUniversitas Gadjah Mada3
Siti MudrikahUniversitas Gadjah Mada2
Dwi Umi SiswantiUniversitas Gadjah Mada2
Elhah Nailul KhasnaState University of Malang2
I. Kade Karisma Gita ArdanaState University of Malang2
Suharti SuhartiState University of Malang2
Nugroho DewayantoUniversitas Gadjah Mada2
Adam AstitiUniversitas Gadjah Mada2
Angga Puja AsianduUniversitas Gadjah Mada2
Ulfah Juniarti SiregarIPB University2
Lucia Tri SuwantiUniversitas Airlangga2
Istini NurafifahUniversitas Gadjah Mada2
Siti ZubaidahState University of Malang2
Susriyati MahanalState University of Malang2
Hendra SusantoState University of Malang2
Abdurrahman Muhammad Fikri RasdiUniversity of Edinburgh1
Novida PratiwiState University of Malang1
Rini RetnosariState University of Malang1
Wisnu Eka WardanaUniversitas Gadjah Mada1
Dea Hastaning TantriUniversitas Gadjah Mada1
Marshanda Nur Roosyana AfifahUniversitas Gadjah Mada1
Hasna Nur AiniUniversitas Gadjah Mada1
‘Ainun Sayyidah ZakiyahState University of Malang1
Choirun Nita FikrianiState University of Malang1
Nina Bunga AnggrainiState University of Malang1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis</dc:title>
	<dc:creator xml:lang="en">Rodiansyah, Achmad </dc:creator>
	<dc:creator xml:lang="en">Mahmudah, Ainul Fitria </dc:creator>
	<dc:creator xml:lang="en">Ulfah, Mastika Marisahani </dc:creator>
	<dc:creator xml:lang="en">Rohmawati, Uun </dc:creator>
	<dc:creator xml:lang="en">Listyorini, Dwi </dc:creator>
	<dc:creator xml:lang="en">Suyono, Eko Agus </dc:creator>
	<dc:creator xml:lang="en">Prabaningtyas, Sitoresmi </dc:creator>
	<dc:description xml:lang="en">Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2021-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/36783</dc:identifier>
	<dc:identifier>10.4308/hjb.28.2.136</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 28 No. 2 (2021): April 2021; 136</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/36783/22014</dc:relation>
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