Melola Discovery

← Melola Discovery

Jurnal SINTA 1

Effect of Ethylene Glycol on Structural Integrity at Each Stage of Preantral Follicle Development Post Vitrification of Rat Ovary-Histological Analysis

HAYATI Journal of Biosciences · Vol. 28 No. 4 (2021) · hlm. 304–311

DOI
10.4308/hjb.28.4.304-311
ISSN
2086-4094, 1978-3019
Terbit
2021-09-29
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

The structure of follicular tissue affects the ability to maintain the structural integrity of follicles against cryoinjury post-vitrification. Histological analysis was conducted on the structural integrity of each stage of preantral follicles post-vitrification using 7.5% and 15.0% doses of ethylene glycol (EG), and ovarian sections with HE staining were observed using an Olympus CX21 microscope connected to Optilab 3.0 lens and Image Raster software. Analysis was conducted on the ovarian cortex in the tracing line area using polygon measure tools to obtain follicle density (follicles/mm2) and follicle index (%) data. The result showed that the EG group 7.5% (KP1) increased follicle density compared to the vitrified group (KKV) in primordial (15.83±1.77) and primary (22.94±8.51) stages. Meanwhile, KP2 (EG 15%) was in primordial (41.92±6.45), primary (11.69±1.95), secondary (33.48±3.63), and tertiary (5.93±0.69) stages. KP1 increased grade 3 follicle index compared to KKV in primary (27.66±2.34), secondary (32.41±6.99), and tertiary (25.00±5.00) stages. Meanwhile, KP2 was in primary (26.87±6.68) and tertiary (25.00±5.00) stages. Both doses of 7.5% and 15.0% EG were able to maintain structural integrity at certain stages of preantral follicles. Secondary and tertiary follicles are the best stages in maintaining grade 3 follicular integrity with the addition of 7.5% EG.

Jaringan kolaborasi para penulis

18 penulis · 45 tautan kepenulisan bersama · 15 lintas organisasi

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • University of Indonesia
  • Universitas Jambi
  • Eijkman Institute for Molecular Biology
  • National Research and Innovation Agency
  • Universitas Trisakti
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Putri Irwanti S.Universitas Jambi1
Ira GustinaUniversity of Indonesia1
David KusmawanUniversitas Jambi1
Silvia Mawarti PerdanaUniversitas Jambi1
Ardi FirmansyahAfiliasi tidak diketahui1
Justus SieversAfiliasi tidak diketahui1
Dionisius DenisEijkman Institute for Molecular Biology1
R. Tedjo SasmonoEijkman Institute for Molecular Biology1
Moudy SorayaNational Research and Innovation Agency1
Sri Widia A. JusmanUniversity of Indonesia1
Ani Retno PrijantiUniversity of Indonesia1
K KartiniUniversitas Trisakti1
Maria EkawatiUniversity of Indonesia1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Effect of Ethylene Glycol on Structural Integrity at Each Stage of Preantral Follicle Development Post Vitrification of Rat Ovary-Histological Analysis</dc:title>
	<dc:creator xml:lang="en">Anita, Nova</dc:creator>
	<dc:creator xml:lang="en">Abinawanto, Abinawanto</dc:creator>
	<dc:creator xml:lang="en">Jusuf, Ahmad Aulia</dc:creator>
	<dc:creator xml:lang="en">Bowolaksono, Anom</dc:creator>
	<dc:creator xml:lang="en">Saoemi, Huriyah Adani</dc:creator>
	<dc:description xml:lang="en">The structure of follicular tissue affects the ability to maintain the structural integrity of follicles against cryoinjury post-vitrification. Histological analysis was conducted on the structural integrity of each stage of preantral follicles post-vitrification using 7.5% and 15.0% doses of ethylene glycol (EG), and ovarian sections with HE staining were observed using an Olympus CX21 microscope connected to Optilab 3.0 lens and Image Raster software. Analysis was conducted on the ovarian cortex in the tracing line area using polygon measure tools to obtain follicle density (follicles/mm2) and follicle index (%) data. The result showed that the EG group 7.5% (KP1) increased follicle density compared to the vitrified group (KKV) in primordial (15.83±1.77) and primary (22.94±8.51) stages. Meanwhile, KP2 (EG 15%) was in primordial (41.92±6.45), primary (11.69±1.95), secondary (33.48±3.63), and tertiary (5.93±0.69) stages. KP1 increased grade 3 follicle index compared to KKV in primary (27.66±2.34), secondary (32.41±6.99), and tertiary (25.00±5.00) stages. Meanwhile, KP2 was in primary (26.87±6.68) and tertiary (25.00±5.00) stages. Both doses of 7.5% and 15.0% EG were able to maintain structural integrity at certain stages of preantral follicles. Secondary and tertiary follicles are the best stages in maintaining grade 3 follicular integrity with the addition of 7.5% EG.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2021-09-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/37807</dc:identifier>
	<dc:identifier>10.4308/hjb.28.4.304-311</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 28 No. 4 (2021): October 2021; 304-311</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/37807/22315</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2021 Nova Anita, Abinawanto Abinawanto, Ahmad Aulia Jusuf, Anom Bowolaksono, Huriyah Adani Saoemi</dc:rights>
</oai_dc:dc>