Melola Discovery

← Melola Discovery

Jurnal SINTA 1

Potential of Conditioned Medium of hATMSCs in Aging Cells Model

HAYATI Journal of Biosciences · Vol. 29 No. 3 (2022) · hlm. 378–388

DOI
10.4308/hjb.29.3.378-388
ISSN
2086-4094, 1978-3019
Terbit
2022-03-15
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Skin aging is caused by the exposure cumulative of ultraviolet radiation, it leads reactive oxygen species (ROS) production in the skin. The conditioned medium of human Adipose Tissue-derived Mesenchymal Stem Cells (hATMSCs) can scavenge free radicals and increase the survival rate of skin cells under oxidative stress. This study examined the protective effects of Conditioned Medium (CM) of hATMSCs in H2O2-induced human skin fibroblast cell line (BJ). The aging cells model using H2O2-induced BJ cells were added CM-hATMSCs in concentrations (0, 10, 30%) and incubated in various time, furthermore BJ cells induced by various H2O2 concentrations (0, 50, 100, 200 µM) incubated for 1 h. The anti-aging potential were measured including viability, ROS and collagen levels in BJ cells which treated CM-hATMSCs. The median inhibitory concentration (IC50) of H2O2 on BJ cells for 1 h incubation was 107.87 μM and 91.25 μM for 10 min incubation. CM-hATMSCs increased the viability on aging model cells. CM-hATMSCs concentration 30% increased the viability of H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs concentration 25% decreased ROS, increased collagen level in H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs increase the viability cells, collagen level and decrease ROS level in aging model cells.

Jaringan kolaborasi para penulis

40 penulis · 216 tautan kepenulisan bersama · 84 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Maranatha Christian University
  • PT Bio Farma (Indonesia)
  • Universitas Prima Indonesia
  • University of Indonesia
  • Padjadjaran University
  • Indonesian Institute of Sciences
  • Bandung Institute of Technology
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Ervi AfifahAfiliasi tidak diketahui13
Ermi GirsangUniversitas Prima Indonesia8
Annisa AmaliaBandung Institute of Technology8
Dhanar Septyawan HadiprasetyoUniversitas Jenderal Achmad Yani7
Tjandrawati MozefIndonesian Institute of Sciences7
Didik PriyandokoIndonesia University of Education7
Rizal AzisUniversity of Indonesia7
Chrismis Novalinda GintingUniversitas Prima Indonesia6
Dwi Davidson RihibihaAfiliasi tidak diketahui6
Harry MurtiAfiliasi tidak diketahui6
Indra BachtiarAfiliasi tidak diketahui6
I Nyoman Ehrich ListerUniversitas Prima Indonesia5
Diana Krisanti JasaputraMaranatha Christian University5
Philips OnggowidjajaMaranatha Christian University5
Ferry SandraUniversitas Trisakti5
Teresa Liliana WargasetiaMaranatha Christian University4
Lusiana DarsonoMaranatha Christian University4
The Fransiska EltaniaMaranatha Christian University4
Wahyu Setia WidodoAfiliasi tidak diketahui4
Hana RatnawatiMaranatha Christian University3
Ika Adhani SholihahBandung Institute of Technology3
Agung NoviantoPadjadjaran University3
Fadhilah Haifa ZahirohAfiliasi tidak diketahui3
Chandra RisdianIndonesian Institute of Sciences2
Jo SuhermanMaranatha Christian University2
SutarnoAfiliasi tidak diketahui1
Iyan HardianaAfiliasi tidak diketahui1
Hesty Utami RamadaniatiAfiliasi tidak diketahui1
Nindya AryantyAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Potential of Conditioned Medium of hATMSCs in Aging Cells Model</dc:title>
	<dc:creator xml:lang="en">Widowati, Wahyu</dc:creator>
	<dc:creator xml:lang="en">Noverina, Rachmawati</dc:creator>
	<dc:creator xml:lang="en">Ayuningtyas, Wireni</dc:creator>
	<dc:creator xml:lang="en">Kurniawan, Dedy</dc:creator>
	<dc:creator xml:lang="en">Arumwardana, Seila</dc:creator>
	<dc:creator xml:lang="en">Kusuma, Hanna Sari W</dc:creator>
	<dc:creator xml:lang="en">Rizal, Rizal</dc:creator>
	<dc:creator xml:lang="en">Laksmitawati, Dian Ratih</dc:creator>
	<dc:creator xml:lang="en">Rinendyaputri, Ratih</dc:creator>
	<dc:creator xml:lang="en">Rilianawati, Rilianawati</dc:creator>
	<dc:creator xml:lang="en">Faried, Ahmad</dc:creator>
	<dc:description xml:lang="en">Skin aging is caused by the exposure cumulative of ultraviolet radiation, it leads reactive oxygen species (ROS) production in the skin. The conditioned medium of human Adipose Tissue-derived Mesenchymal Stem Cells (hATMSCs) can scavenge free radicals and increase the survival rate of skin cells under oxidative stress. This study examined the protective effects of Conditioned Medium (CM) of hATMSCs in H2O2-induced human skin fibroblast cell line (BJ). The aging cells model using H2O2-induced BJ cells were added CM-hATMSCs in concentrations (0, 10, 30%) and incubated in various time, furthermore BJ cells induced by various H2O2 concentrations (0, 50, 100, 200 µM) incubated for 1 h. The anti-aging potential were measured including viability, ROS and collagen levels in BJ cells which treated CM-hATMSCs. The median inhibitory concentration (IC50) of H2O2 on BJ cells for 1 h incubation was 107.87 μM and 91.25 μM for 10 min incubation. CM-hATMSCs increased the viability on aging model cells. CM-hATMSCs concentration 30% increased the viability of H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs concentration 25% decreased ROS, increased collagen level in H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs increase the viability cells, collagen level and decrease ROS level in aging model cells.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2022-03-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/38598</dc:identifier>
	<dc:identifier>10.4308/hjb.29.3.378-388</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 29 No. 3 (2022): May 2022; 378-388</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/38598/23156</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Wahyu Widowati, Rachmawati Noverina, Wireni Ayuningtyas, Dedy Kurniawan, Seila Arumwardana, Hanna Sari W Kusuma, Rizal Rizal, Dian Ratih Laksmitawati, Ratih Rinendyaputri, Rilianawati Rilianawati, Ahmad Faried</dc:rights>
</oai_dc:dc>