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In Vitro Pancreatic Lipase Inhibition by Marine Fungi Purpureocillium lilacinum Associated with Stylissa sp. Sponge as Anti-obesity Agent

HAYATI Journal of Biosciences · Vol. 29 No. 1 (2021) · hlm. 76–86

DOI
10.4308/hjb.29.1.76-86
ISSN
2086-4094, 1978-3019
Terbit
2021-12-09
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

This study aimed to evaluate the potential of marine fungus Purpureocillium lilacinum isolated from an Indonesian marine sponge Stylissa sp. as an anti-obesity agent through pancreatic lipase inhibition assay. The fungus was identified as P. lilacinum through morphological and molecular characteristics. The fungal extract’s inhibition activity and kinetics were evaluated using spectrophotometry and Lineweaver-Burk plots. Ethyl acetate and butanol were used for extraction. Both extracts showed pancreatic lipase inhibition in a concentration-dependent manner. Both crude extracts were then fractionated once. All fractionated extracts showed inhibitory activity above 50%, with the highest activity found in fraction 5 of ethyl acetate at 93.41% inhibition. The best fractionated extract had an IC50value of 220.60 µg.mL-1. The most active fraction of P. lilacinum had a competitive-type inhibitor behavior as shown by the value of Vmax not significantly changing from 388.80 to 382.62 mM pNP.min-1, and the Michaelis-Menten constant (KM) increased from 2.02 to 5.47 mM in the presence of 500 µg.mL-1 fractionated extract. Metabolite identification with LC-MS/MS QTOF suggested that galangin, kaempferol, and quercetin were responsible for the observed lipase inhibition.

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  • IPB University
  • Indonesian Institute of Sciences
  • Atma Jaya Catholic University of Indonesia
  • University of Hawaiʻi at Mānoa
  • Syarif Hidayatullah State Islamic University Jakarta
  • Universitas Islam Negeri Alauddin Makassar
  • State University of Jakarta
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Rekan penulisOrganisasiArtikel bersama
Agik SuprayogiIPB University5
Novik NurhidayatIndonesian Institute of Sciences4
Triadiati TriadiatiIPB University4
Irmanida BatubaraIPB University4
Sri ListiyowatiIPB University3
Yohana Caecilia SulistyaningsihAfiliasi tidak diketahui3
Yasinta Ratna Esti WulandariAtma Jaya Catholic University of Indonesia3
Utut WidyastutiIPB University3
Latifah Kosim DarusmanIPB University3
Rohani Cinta Badia GintingIPB University3
Henny PurwaningsihIPB University2
Ali Aulia GhozaliIPB University2
La Ode SumarlinSyarif Hidayatullah State Islamic University Jakarta2
Aryani Sismin SatyaningtijasIPB University2
Rut Novalia Rahmawati SianiparIPB University2
Kartini KramadibrataIndonesian Institute of Sciences2
Adi YulandiAtma Jaya Catholic University of Indonesia2
Wulan Tri WahyuniIPB University2
Sihegiko KanayaNara Institute of Science and Technology2
Alfiah AlifAfiliasi tidak diketahui1
Amalyah FebryantiUniversitas Islam Negeri Alauddin Makassar1
Hajar HajarAfiliasi tidak diketahui1
Meyliana WulandariState University of Jakarta1
Kemala S. NagurIPB University1
SIHEGIKO KANAYAAfiliasi tidak diketahui1
SukarnoAfiliasi tidak diketahui1
Partomuan SimanjuntakIndonesian Institute of Sciences1
Eris SeptianaIPB University1
Sri BudiartiIPB University1
Yulin LestariIPB University1
Budi WirawanIPB University1
Fitriani IndahsariIPB University1
Akhiruddin MadduIPB University1
Sri SugiartiIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">In Vitro Pancreatic Lipase Inhibition by Marine Fungi Purpureocillium lilacinum Associated with Stylissa sp. Sponge as Anti-obesity Agent </dc:title>
	<dc:creator xml:lang="en">Fadillah, Wendi Nurul</dc:creator>
	<dc:creator xml:lang="en">Sukarno, Nampiah</dc:creator>
	<dc:creator xml:lang="en">Iswantini, Dyah</dc:creator>
	<dc:creator xml:lang="en">Rahminiwati, Min</dc:creator>
	<dc:creator xml:lang="en">Hanif, Novriyandi</dc:creator>
	<dc:creator xml:lang="en">Waite, Mashuri</dc:creator>
	<dc:description xml:lang="en">This study aimed to evaluate the potential of marine fungus Purpureocillium lilacinum isolated from an Indonesian marine sponge Stylissa sp. as an anti-obesity agent through pancreatic lipase inhibition assay. The fungus was identified as P. lilacinum through morphological and molecular characteristics. The fungal extract’s inhibition activity and kinetics were evaluated using spectrophotometry and Lineweaver-Burk plots. Ethyl acetate and butanol were used for extraction. Both extracts showed pancreatic lipase inhibition in a concentration-dependent manner. Both crude extracts were then fractionated once. All fractionated extracts showed inhibitory activity above 50%, with the highest activity found in fraction 5 of ethyl acetate at 93.41% inhibition. The best fractionated extract had an IC50value of 220.60 µg.mL-1. The most active fraction of P. lilacinum had a competitive-type inhibitor behavior as shown by the value of Vmax not significantly changing from 388.80 to 382.62 mM pNP.min-1, and the Michaelis-Menten constant (KM) increased from 2.02 to 5.47 mM in the presence of 500 µg.mL-1 fractionated extract. Metabolite identification with LC-MS/MS QTOF suggested that galangin, kaempferol, and quercetin were responsible for the observed lipase inhibition.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2021-12-09</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/38831</dc:identifier>
	<dc:identifier>10.4308/hjb.29.1.76-86</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 29 No. 1 (2022): January 2022; 76-86</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/38831/22584</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Wendi Nurul Fadillah, Nampiah Sukarno, Dyah Iswantini, Min Rahminiwati, Novriyandi Hanif, Mashuri Waite</dc:rights>
</oai_dc:dc>