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Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia

HAYATI Journal of Biosciences · Vol. 30 No. 1 (2022) · hlm. 53–64

DOI
10.4308/hjb.30.1.53-64
ISSN
2086-4094, 1978-3019
Terbit
2022-08-19
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

This study was aimed to confirms the Banana Bunchy Top Virus (BBTV) infection of ten banana accessions from Indonesia through PCR assay using primer of BBTV coat protein (CP) gene, also the sequences characteristics and phylogeny. Preliminary morphological results showed five acessions were positively infected with slight to severe intensity symptoms i.e. Pisang Berlin, Candi, Billa, Morosebo, and Mas Kripik; and five accessions were symptomless i.e. M. acuminata var. rutilifes and M. balbisiana, Pisang Ebung, Madu and Moseng. However, PCR results qualitatively confirms that all accessions were positively infected. Two sizes of amplicons were identified. Pisang Candi isolate was showing ±500 bp amplicons, whereas the others were ±1000-1100 bp. The total aligned and selected BBTV CP sequences of 36 accessions (bananas and other host species) was 450 nt. It was considered highly conserved (85.55%), low G+C content (41%), and high genetic similarities (92.47 to 100%). Phylogenetic analysis delineates isolates into two large groups, i.e. the Asian group (East Asia and South-east Asia) and the South Pacific group (South Asia, Africa and the Pacific). The findings of this study are usefull for broader applicability of further banana breeding program particularly for mitigation, selection and evaluation of banana with BBTV resistance.

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  • Indonesian Institute of Sciences
  • Universitas Gadjah Mada
  • Jenderal Soedirman University
  • National Research and Innovation Agency
  • Amélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales
  • The University of Queensland
  • Universitas Islam Negeri Maulana Malik Ibrahim
  • Organisasi lain
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  • Organisasi sama
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Endang MugiastutiJenderal Soedirman University5
Loekas SoesantoJenderal Soedirman University5
Shofiyatul Mas’udahIndonesian Institute of Sciences3
Arif WibowoUniversitas Gadjah Mada3
Tri JokoUniversitas Gadjah Mada3
Alan SoffanUniversitas Gadjah Mada3
Setyawan Agung DanartoIndonesian Institute of Sciences2
Janis DamaiyaniIndonesian Institute of Sciences2
Abdul MananJenderal Soedirman University2
Siti NurfadilahIndonesian Institute of Sciences2
Ruly Eko Kusuma KurniawanJenderal Soedirman University2
Elok Rifqi FirdianaNational Research and Innovation Agency2
Dewi LestariNational Research and Innovation Agency2
Ratna Stia DewiJenderal Soedirman University2
Ani WidiastutiUniversitas Gadjah Mada2
Stephen Mark HarperThe University of Queensland2
Arif WibowoUniversitas Gadjah Mada2
Indah NurainiUniversitas Gadjah Mada2
Didik WahyudiUniversitas Islam Negeri Maulana Malik Ibrahim2
TrimantoAfiliasi tidak diketahui1
Sugeng BudihartaIndonesian Institute of Sciences1
TITUT YULISTYARINIIndonesian Institute of Sciences1
Rachmawan Adi LaksonoIndonesian Institute of Sciences1
Nurlaily LaviantiAfiliasi tidak diketahui1
Bekti YunantoAfiliasi tidak diketahui1
Trimanto TrimantoAfiliasi tidak diketahui1
Fauziah FauziahIndonesian Institute of Sciences1
Dewi Ayu LestariIndonesian Institute of Sciences1
Ridesti RindyastutiIndonesian Institute of Sciences1
Ilham Kurnia AbywijayaIndonesian Institute of Sciences1
Jaka WidadaUniversitas Gadjah Mada1
Nur Fitriana AriyantiUniversitas Gadjah Mada1
Nurtiati NurtiatiJenderal Soedirman University1
Tri Rini NuringtyasUniversitas Gadjah Mada1
Junaidi JunaidiAmélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales1
Anne Clément‐VidalAmélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales1
Éric GohetABSys1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia</dc:title>
	<dc:creator xml:lang="en">Hapsari, Lia</dc:creator>
	<dc:creator xml:lang="en">Rahayuniati, Ruth Feti</dc:creator>
	<dc:creator xml:lang="en">Subandiyah, Siti</dc:creator>
	<dc:description xml:lang="en">This study was aimed to confirms the Banana Bunchy Top Virus (BBTV) infection of ten banana accessions from Indonesia through PCR assay using primer of BBTV coat protein (CP) gene, also the sequences characteristics and phylogeny. Preliminary morphological results showed five acessions were positively infected with slight to severe intensity symptoms i.e. Pisang Berlin, Candi, Billa, Morosebo, and Mas Kripik; and five accessions were symptomless i.e. M. acuminata var. rutilifes and M. balbisiana, Pisang Ebung, Madu and Moseng. However, PCR results qualitatively confirms that all accessions were positively infected. Two sizes of amplicons were identified. Pisang Candi isolate was showing ±500 bp amplicons, whereas the others were ±1000-1100 bp. The total aligned and selected BBTV CP sequences of 36 accessions (bananas and other host species) was 450 nt. It was considered highly conserved (85.55%), low G+C content (41%), and high genetic similarities (92.47 to 100%). Phylogenetic analysis delineates isolates into two large groups, i.e. the Asian group (East Asia and South-east Asia) and the South Pacific group (South Asia, Africa and the Pacific). The findings of this study are usefull for broader applicability of further banana breeding program particularly for mitigation, selection and evaluation of banana with BBTV resistance.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2022-08-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/41521</dc:identifier>
	<dc:identifier>10.4308/hjb.30.1.53-64</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 30 No. 1 (2023): January 2023; 53-64</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/41521/23972</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Lia Hapsari, Ruth Feti Rahayuniati, Siti Subandiyah</dc:rights>
</oai_dc:dc>