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The Patterns of Restriction Fragment of Several Enzymes to Distinguish Toxoplasma gondii Isolates Virulent and Avirulent Strains using GRA1 and GRA7 Genetic Marker

HAYATI Journal of Biosciences · Vol. 30 No. 4 (2023) · hlm. 725–733

DOI
10.4308/hjb.30.4.725-733
ISSN
2086-4094, 1978-3019
Terbit
2023-04-17
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of the locus has been carried out to determine the different genotypes of T. gondii (strain types I, II, and III) that are associated with human toxoplasmosis. The several genotypes of T. gondii (strain types I, II, and III) that are linked to human toxoplasmosis have been identified through genetic study of the locus. In this investigation, PCR-RFLP was found to be a useful, and simple method genotypic characterization. The objective of this study was to genotyping characterize T. gondii RH and BEV strains isolates by PCR-RFLP using several restriction enzymes. T. gondii tachyzoite DNA was extracted and amplified by PCR using dense granule genetic markers (GRA1 and GRA7) designed with Primer3plus. The amplification were digested using the restriction enzymes. The PCR-RFLP amplified dense granule products was used to classify strains into two genotypes of T. gondii (virulent and avirulent). The results demonstrated that the RFLP patterns of the GRA1 and GRA7 gene area digested by DdeI, MvaI, HinfI, RsaI, and Sau96I enzymes can be used to identify virulent or avirulent strains of T. gondii. Toxoplasma gondii RH and BEV strain produced different digestion product which can be used to distinguished the strains.

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Tutik WresdiyatiAfiliasi tidak diketahui2
Wayan T. ArtamaAfiliasi tidak diketahui2
Agus WiyonoAfiliasi tidak diketahui2
Ghinaa Zain NabiilahBinus University1
Islam Nur AlamBinus University1
Muhammad Fadlan HidayatBinus University1
Risa TiuriaIPB University1
Arif Rahman JabalUniversity of Palangka Raya1
Arini RatnasariHasanuddin University1
Isrok Malikus SufiAfiliasi tidak diketahui1
Etih SudarnikaAfiliasi tidak diketahui1
Adi WinartoAfiliasi tidak diketahui1
VENNY FEBRIYANIAfiliasi tidak diketahui1
Dwi PriyowidodoAfiliasi tidak diketahui1
Mukh Fajar NasrullohAfiliasi tidak diketahui1
Raden Wisnu NurcahyoAfiliasi tidak diketahui1
Lintang Winantya FirdausyAfiliasi tidak diketahui1
Arifin NugrahaAfiliasi tidak diketahui1
Ni Nyoman Ayu DewiAfiliasi tidak diketahui1
Yulia SariAfiliasi tidak diketahui1
Jarot SubandonoAfiliasi tidak diketahui1
Soenarwan Hery PoerwantoAfiliasi tidak diketahui1
R IndrianiAfiliasi tidak diketahui1
N.L.P.I. DharmayantiAfiliasi tidak diketahui1
A WiyonoAfiliasi tidak diketahui1
R.M.A AdjidAfiliasi tidak diketahui1
Eni KusumaningtyasAfiliasi tidak diketahui1
D FL FitaningtyasAfiliasi tidak diketahui1
. DARMINTOAfiliasi tidak diketahui1
I. YuniartoAfiliasi tidak diketahui1
. SulinawatiAfiliasi tidak diketahui1
H. SusianiAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">The Patterns of Restriction Fragment of Several Enzymes to Distinguish Toxoplasma gondii Isolates Virulent and Avirulent Strains using GRA1 and GRA7 Genetic Marker</dc:title>
	<dc:creator xml:lang="en">Ekawasti, Fitrine</dc:creator>
	<dc:creator xml:lang="en">Purwanto, Eko Setyo</dc:creator>
	<dc:creator xml:lang="en">Nepho, Farlin</dc:creator>
	<dc:creator xml:lang="en">Kurniawati, Dyah Ayu</dc:creator>
	<dc:creator xml:lang="en">Subekti, Didik Tulus</dc:creator>
	<dc:creator xml:lang="en">Damayanti, Rini</dc:creator>
	<dc:creator xml:lang="en">Sa’diah, Siti</dc:creator>
	<dc:creator xml:lang="en">Cahyaningsih, Umi</dc:creator>
	<dc:description xml:lang="en">Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of the locus has been carried out to determine the different genotypes of T. gondii (strain types I, II, and III) that are associated with human toxoplasmosis. The several genotypes of T. gondii (strain types I, II, and III) that are linked to human toxoplasmosis have been identified through genetic study of the locus. In this investigation, PCR-RFLP was found to be a useful, and simple method genotypic characterization. The objective of this study was to genotyping characterize T. gondii RH and BEV strains isolates by PCR-RFLP using several restriction enzymes. T. gondii tachyzoite DNA was extracted and amplified by PCR using dense granule genetic markers (GRA1 and GRA7) designed with Primer3plus. The amplification were digested using the restriction enzymes. The PCR-RFLP amplified dense granule products was used to classify strains into two genotypes of T. gondii (virulent and avirulent). The results demonstrated that the RFLP patterns of the GRA1 and GRA7 gene area digested by DdeI, MvaI, HinfI, RsaI, and Sau96I enzymes can be used to identify virulent or avirulent strains of T. gondii. Toxoplasma gondii RH and BEV strain produced different digestion product which can be used to distinguished the strains.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2023-04-17</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/44778</dc:identifier>
	<dc:identifier>10.4308/hjb.30.4.725-733</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 30 No. 4 (2023): July 2023; 725-733</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/44778/25414</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Fitrine Ekawasti, Eko Setyo Purwanto, Farlin Nepho, Dyah Ayu Kurniawati, Didik Tulus Subekti, Rini Damayanti, Siti Sa’diah, Umi Cahyaningsih</dc:rights>
</oai_dc:dc>