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Lysinibacillus sphaericus Isolated from Palm Oil Waste Land as Lipase Producer

HAYATI Journal of Biosciences · Vol. 31 No. 3 (2024) · hlm. 425–431

DOI
10.4308/hjb.31.3.425-431
ISSN
2086-4094, 1978-3019
Terbit
2024-01-05
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

In this research, lipolyic bacteria have been isolated from palm oil waste land for the production of lipase. Species of potential lipolytic bacteria were identified based on their morphology and sequences of 16 rRNA gene. Enzymes are produced by growing bacteria in a medium with various vegetable oils and nitrogen sources. The enzyme produced by the bacteria measured its lipolytic activity against the substrate para-nitrophenylpalmitate. The lipolytic bacteria was recognized as Lysinibacillus sphaericus L49a based on morphological and phylogenetic analysis. Mineral media with different vegetable oils as carbon sources, and different nitrogen sources were suitable for growth and production of lipase enzymes of L. sphaericus L49a. Culltivation of L. sphaericus L49a in medium containing ammonium sulfate and olive oil produced lipase with the highest lipolytic activity.

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IwanAfiliasi tidak diketahui1
Usman BetawiAfiliasi tidak diketahui1
Muhammad Iqbal Hanafi NasutionUniversitas Islam Negeri Sumatera Utara1
Ni'matuzahrohAfiliasi tidak diketahui1
Salamun -Afiliasi tidak diketahui1
Muhammad Fath Alhaqqi Sanis SalamyAfiliasi tidak diketahui1
Nabilatun NisaUniversitas Airlangga1
Farah Aisyah NafidiastriUniversitas Negeri Surabaya1
Rizky Danang SusetyoUniversitas Airlangga1
Hakimatul HusniyahUniversitas Airlangga1
Almando GeraldiUniversitas Airlangga1
Dewi SukmaIPB University1
RahayuAfiliasi tidak diketahui1
Yang Geun MoDankook University1
Eun Ji BaeAfiliasi tidak diketahui1
Febriyanti Nur AmaniNational Research and Innovation Agency1
Apon Zaenal MustopaNational Research and Innovation Agency1
Rika Indri AstutiIPB University1
Baso ManguntungiUniversitas Sulawesi Barat1
Ai HertatiNational Research and Innovation Agency1
Rifqiyah Nur UmamiNational Research and Innovation Agency1
Sheila ChairunnisaNational Research and Innovation Agency1
Nurlaili EkawatiNational Research and Innovation Agency1
Jendri MamangkeyUniversitas Kristen Indonesia1
Tatenda Calvin ChihomboriIPB University1
Ilma MutiaraUniversitas Teknologi Sumbawa1
Redoyan RefliNational Research and Innovation Agency1
Herman IrawanNational Research and Innovation Agency1
Joko Pebrianto TrinugrohoNational Research and Innovation Agency1
Rizna AkmaliyahIPB University1
Sapto AndriyonoUniversitas Airlangga1
Sri Puji Astuti WahyuningsihUniversitas Airlangga1
Hoang Dang Khoa DoTrường ĐH Nguyễn Tất Thành1
Nurul Latifatul AiniUniversitas Airlangga1
Muhammad BachruddinUniversitas Airlangga1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Lysinibacillus sphaericus Isolated from Palm Oil Waste Land as Lipase Producer</dc:title>
	<dc:creator xml:lang="en">Sumarsih, Sri </dc:creator>
	<dc:creator xml:lang="en">Sofijan Hadi</dc:creator>
	<dc:creator xml:lang="en">Fatimah</dc:creator>
	<dc:creator xml:lang="en">Rizka Diah Fitri</dc:creator>
	<dc:creator xml:lang="en">Gilva Illavi</dc:creator>
	<dc:description xml:lang="en">In this research, lipolyic bacteria have been isolated from palm oil waste land for the production of lipase. Species of potential lipolytic bacteria were identified based on their morphology and sequences of 16 rRNA gene. Enzymes are produced by growing bacteria in a medium with various vegetable oils and nitrogen sources. The enzyme produced by the bacteria measured its lipolytic activity against the substrate para-nitrophenylpalmitate. The lipolytic bacteria was recognized as Lysinibacillus sphaericus L49a based on morphological and phylogenetic analysis. Mineral media with different vegetable oils as carbon sources, and different nitrogen sources were suitable for growth and production of lipase enzymes of L. sphaericus L49a. Culltivation of L. sphaericus L49a in medium containing ammonium sulfate and olive oil produced lipase with the highest lipolytic activity.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2024-01-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/49662</dc:identifier>
	<dc:identifier>10.4308/hjb.31.3.425-431</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 31 No. 3 (2024): May 2024; 425-431</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/49662/27215</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Sri  Sumarsih, Sofijan Hadi, Fatimah, Rizka Diah Fitri, Gilva Illavi</dc:rights>
</oai_dc:dc>