Melola Discovery

← Melola Discovery

Jurnal SINTA 1

Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells: A Preliminary Investigation into Vitiligo Treatment

HAYATI Journal of Biosciences · Vol. 32 No. 1 (2024) · hlm. 203–211

DOI
10.4308/hjb.32.1.203-211
ISSN
2086-4094, 1978-3019
Terbit
2024-11-15
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Black cumin (Nigella sativa) is a herbal plant that has been cultivated locally in Indonesia and is traditionally used for various diseases. Thymoquinone, one of the main components, is rich in biological activity. In several countries, topical application of its oil on human skin with vitiligo can stimulate skin repigmentation with minimal side effects. This study aims to determine the viability of B16F10 melanoma mouse cells against ethanol and n-hexane extracts of black cumin seeds through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ethanol extract (EE) yield was 14.306%, and the n-hexane extract (NHE) was 7.442%. Phytochemical screening of EE detected flavonoids, alkaloids, saponins, and steroids, and High-performance liquid chromatography (HPLC) detected 0.040% thymoquinone. The MTT test showed cell viability was >100% from EE at all treatment concentrations, namely 0.75-100 ppm and only 0.75-6.25 ppm for NHE. In conclusion, this study indicates that 96% EE of Nigella sativa is less toxic than NHE on B16F10 mouse melanoma cells and has potential as an alternative treatment for vitiligo, which needs to be proven in further research.

Jaringan kolaborasi para penulis

40 penulis · 93 tautan kepenulisan bersama · 15 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • IPB University
  • Universitas Halu Oleo
  • Syarif Hidayatullah State Islamic University Jakarta
  • State University of Semarang
  • Indonesian Agency for Agricultural Research and Development
  • Indonesian Institute of Sciences
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Ono SuparnoIPB University2
. YulianaAfiliasi tidak diketahui2
Huda Shalahudin DarusmanIPB University2
Bambang Pontjo PriosoeryantoIPB University2
Ietje WientarsihIPB University2
Shalina SebayangIPB University2
Nurizka KhoeraniAfiliasi tidak diketahui1
Syandra SariAfiliasi tidak diketahui1
Ramadhan TosepuUniversitas Halu Oleo1
Fithria FithriaUniversitas Halu Oleo1
Sartiah YusranAfiliasi tidak diketahui1
Marimin MariminAfiliasi tidak diketahui1
Reny Nur RohmahAfiliasi tidak diketahui1
I SubandrioAfiliasi tidak diketahui1
LisdianaState University of Semarang1
Wulan ChristijantiAfiliasi tidak diketahui1
Titi Candra SunartiIPB University1
Akhmad Endang Zainal HasanIPB University1
Djumali MangunwidjajaIPB University1
Irma Herawati SupartoIPB University1
Uus SaepulohIPB University1
Rizki Aulia AnsariIPB University1
Rachmitasari NovianaIPB University1
Mia AudinaIPB University1
Bella Fatima Dora ZaelaniIPB University1
Ekowati HandharyaniIPB University1
Hapsari MahatmiAfiliasi tidak diketahui1
SumiatiAfiliasi tidak diketahui1
Elizabeth WinaIndonesian Agency for Agricultural Research and Development1
T ToharmatIPB University1
Yenni YusrianiAfiliasi tidak diketahui1
Upik Kesumawati HadiIPB University1
Dwi Hery AstutiIndonesian Institute of Sciences1
Wiwin WinarsihIPB University1
Eko Prasetyo NugrohoIPB University1
Amalia AfifahAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo Treatment</dc:title>
	<dc:creator xml:lang="en">Pratiwi, Dian</dc:creator>
	<dc:creator xml:lang="en">Mariya, Silmi</dc:creator>
	<dc:creator xml:lang="en">Rayendra, Raendi</dc:creator>
	<dc:creator xml:lang="en">Setiyono, Agus</dc:creator>
	<dc:description xml:lang="en">Black cumin (Nigella sativa) is a herbal plant that has been cultivated locally in Indonesia and is traditionally used for various diseases. Thymoquinone, one of the main components, is rich in biological activity. In several countries, topical application of its oil on human skin with vitiligo can stimulate skin repigmentation with minimal side effects. This study aims to determine the viability of B16F10 melanoma mouse cells against ethanol and n-hexane extracts of black cumin seeds through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ethanol extract (EE) yield was 14.306%, and the n-hexane extract (NHE) was 7.442%. Phytochemical screening of EE detected flavonoids, alkaloids, saponins, and steroids, and High-performance liquid chromatography (HPLC) detected 0.040% thymoquinone. The MTT test showed cell viability was &amp;gt;100% from EE at all treatment concentrations, namely 0.75-100 ppm and only 0.75-6.25 ppm for NHE. In conclusion, this study indicates that 96% EE of Nigella sativa is less toxic than NHE on B16F10 mouse melanoma cells and has potential as an alternative treatment for vitiligo, which needs to be proven in further research.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2024-11-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/54670</dc:identifier>
	<dc:identifier>10.4308/hjb.32.1.203-211</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 32 No. 1 (2025): January 2025; 203-211</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/54670/29413</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Dian Pratiwi, Silmi Mariya, Raendi Rayendra, Agus Setiyono</dc:rights>
</oai_dc:dc>