Melola Discovery

← Melola Discovery

Jurnal SINTA 1

Characteristics of Nitrogen-Fixing Bacteria Isolated from the Rhizosphere at Kaolin Lake, Belitung, Indonesia

HAYATI Journal of Biosciences · Vol. 32 No. 3 (2025) · hlm. 611–622

DOI
10.4308/hjb.32.3.611-622
ISSN
2086-4094, 1978-3019
Terbit
2025-01-24
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

Former kaolin mining has poor physical and chemical properties for plants. The low nutrient content makes difficult for plants to grow. Nitrogen (N2) is an important macronutrient for plants, but it can only be absorbed in the form of ammonium ions (NH4+) or nitrate ions (NO3-­). Therefore, it is necessary to transform nitrogen, which is able to change the molecular form so that it can be absorbed by plants. The strategy that can be done is using nitrogen-fixing bacteria. This study aimed to explore and analyze the diversity of nitrogen-fixing bacteria based on 16S rRNA and nifH gene from the rhizosphere of the Kaolin Lake area. The method started with bacterial isolation from rhizosphere soil samples. Selected isolates were tested for ammonium content to determine the ability of isolates to fix nitrogen. In this study, 13 isolates had the ability to fix nitrogen, two of which had a high ammonium concentration, namely RBN 5.6, and RBA 2.3. The highest ammonium concentration was produced by isolate RBN5.6 amounting to 7.55 μg/ml. Based on 16S rRNA gene identification, isolate RBN5.6 was similar to Burkholderia cepacia and isolate RBA2.3 was similar to Bacillus aquimaris. Based on the detection of the nifH gene, isolate RBN5.6 was identified as a protein-encoding dinitrogenase reductase gene group cluster from the Stutzerimonas stutzeri.

Jaringan kolaborasi para penulis

40 penulis · 115 tautan kepenulisan bersama · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • IPB University
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Nisa Rachmania MubarikAfiliasi tidak diketahui6
Antonius SuwantoAfiliasi tidak diketahui6
Anja MeryandiniAfiliasi tidak diketahui5
Aris Tri WahyudiIPB University5
Sri ListiyowatiAfiliasi tidak diketahui3
SRI BUDIARTIAfiliasi tidak diketahui3
Sipriyadi SipriyadiAfiliasi tidak diketahui2
Risky Hadi WibowoAfiliasi tidak diketahui2
Maggy Thenawidjaya SuhartonoAfiliasi tidak diketahui2
Hamim HamimAfiliasi tidak diketahui2
Cecilia Anna SeumahuAfiliasi tidak diketahui2
Dedy Duryadi SolihinAfiliasi tidak diketahui2
Alina AkhdiyaAfiliasi tidak diketahui2
ESTI PUSPITASARIAfiliasi tidak diketahui2
Andreas Adhi SatyaAfiliasi tidak diketahui2
Yepy Hardi RustamAfiliasi tidak diketahui2
Apon Zaenal MustopaAfiliasi tidak diketahui2
Baso ManguntungiAfiliasi tidak diketahui2
Dian HendrayantiAfiliasi tidak diketahui1
Dwi Andreas SantosaAfiliasi tidak diketahui1
ALINA AKHADIYAAfiliasi tidak diketahui1
Anik KusmiatunAfiliasi tidak diketahui1
DONDIN SAJUTHIAfiliasi tidak diketahui1
SUSAN SOKAAfiliasi tidak diketahui1
DIAH ISKANDRIATIAfiliasi tidak diketahui1
KATHARINA JESSICAAfiliasi tidak diketahui1
Yulin LestariAfiliasi tidak diketahui1
. MahyarudinAfiliasi tidak diketahui1
RIKA RAFFIUDINAfiliasi tidak diketahui1
Andri FerbiyantoAfiliasi tidak diketahui1
Zulhaerati ZulhaeratiAfiliasi tidak diketahui1
Yuli Siti FatmaAfiliasi tidak diketahui1
Yuni Puji HastutiAfiliasi tidak diketahui1
Rizal Khoirun AlfisahAfiliasi tidak diketahui1
Nurfadhilla RahmadhaniAfiliasi tidak diketahui1
Nabila Tsoerayya Gustia PudjasAfiliasi tidak diketahui1
Lisdar I SudirmanAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Characteristics of Nitrogen-Fixing Bacteria Isolated from the Rhizosphere at Kaolin Lake, Belitung, Indonesia</dc:title>
	<dc:creator xml:lang="en">Nurhakiki</dc:creator>
	<dc:creator xml:lang="en">Rusmana, Iman</dc:creator>
	<dc:creator xml:lang="en">Astuti, Rika Indri</dc:creator>
	<dc:description xml:lang="en">Former kaolin mining has poor physical and chemical properties for plants. The low nutrient content makes difficult for plants to grow. Nitrogen (N2) is an important macronutrient for plants, but it can only be absorbed in the form of ammonium ions (NH4+) or nitrate ions (NO3-­). Therefore, it is necessary to transform nitrogen, which is able to change the molecular form so that it can be absorbed by plants. The strategy that can be done is using nitrogen-fixing bacteria. This study aimed to explore and analyze the diversity of nitrogen-fixing bacteria based on 16S rRNA and nifH gene from the rhizosphere of the Kaolin Lake area. The method started with bacterial isolation from rhizosphere soil samples. Selected isolates were tested for ammonium content to determine the ability of isolates to fix nitrogen. In this study, 13 isolates had the ability to fix nitrogen, two of which had a high ammonium concentration, namely RBN 5.6, and RBA 2.3. The highest ammonium concentration was produced by isolate RBN5.6 amounting to 7.55 μg/ml. Based on 16S rRNA gene identification, isolate RBN5.6 was similar to Burkholderia cepacia and isolate RBA2.3 was similar to Bacillus aquimaris. Based on the detection of the nifH gene, isolate RBN5.6 was identified as a protein-encoding dinitrogenase reductase gene group cluster from the Stutzerimonas stutzeri.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2025-01-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/59430</dc:identifier>
	<dc:identifier>10.4308/hjb.32.3.611-622</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 32 No. 3 (2025): May 2025; 611-622</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/59430/30051</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Nurhakiki, Iman Rusmana, Rika Indri Astuti</dc:rights>
</oai_dc:dc>