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The Potential of Cytotoxin and Antiviral in Sargassum polycystum and Sargassum ilicifolium’s Polysaccharides Extract

ILMU KELAUTAN: Indonesian Journal of Marine Sciences · Vol. 25 No. 3 (2020) · hlm. 91–96

DOI
10.14710/ik.ijms.25.3.91-96
ISSN
2406-7598, 0853-7291
Terbit
2020-09-02
Penerbit
Marine Science Department Diponegoro University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Marine algae known as one producers of bioactive compounds. This study aims to analyze the cytotoxicity and antiviral activity in Sargassum polycystum and Sargassum ilicifolium tested with Herpes Simplex Virus (HSV). The polysaccharides extract of algae was used in this study, as sulfated polysaccharides have been reported has bioactivity. Cytotoxicity either antiviral could be correlated with the sulfate content as well as nature and chemical composition of the polysaccharides. Cytotoxicity and antiviral analysis based upon cell viability. Using the Vero cell / HSV-1 model, cytotoxicity was evaluated by incubating cellular suspensions (3.5×105 cells.mL-1) with various dilutions (concentration from 1 to 500 µg.mL-1, four wells per concentration) of fractions in 96-well plates (72h, 37°C, 5% CO2) in Eagle's MEM containing 8% FCS. The cells were examined daily under a phase-contrast microscope to determine the minimum concentration of hydrolysate dry matter that induced alterations in cell morphology, including swelling, shrinkage, granularity and detachment. Algae S. illicifolium was found to have the highest cytotoxic content in each solution compared to S. polycystum. Algae S. illicifolium in KOH 4M (cellulose) reached 2,707 µg.ml-1, then HCl pH 2 (fucoidan) was 2,477 µg.ml-1, then CaCl2 2% (fucoidan) was 2,362 µg.ml-1, and in Na2CO3 3% (alginates) was 2,134 µg.ml-1. For antiviral, S. polycystum contained the highest antiviral compounds compared to S. illicifolium with KOH 4M (cellulose) solution was reached 67.02 µg.ml-1. Then in Na2CO3 3% (alginates) which was 33.25 µg.ml-1, then CaCl2 2% (fucoidan) which was 31.62 µg.ml-1,and HCl pH 2 (fucoidan) was 30.08 µg.ml-1. After all, the highest bioactivity compounds was found with KOH 4M (cellulose) for cytotoxicity in S. ilicifolium and antiviral activity in S. polycystum.

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  • Diponegoro University
  • Universitas Halu Oleo
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Rekan penulisOrganisasiArtikel bersama
OCKY KARNA RADJASAAfiliasi tidak diketahui6
Ambariyanto AmbariyantoAfiliasi tidak diketahui5
Agus SabdonoDiponegoro University4
Ervia YudiatiDiponegoro University4
Tri Winarni AgustiniAfiliasi tidak diketahui3
Nuril AzharAfiliasi tidak diketahui2
Retno HartatiAfiliasi tidak diketahui2
Djoko SupraptoAfiliasi tidak diketahui2
Retno MurwaniAfiliasi tidak diketahui2
Subagiyo SubagiyoAfiliasi tidak diketahui2
Khoeruddin WittriansyahAfiliasi tidak diketahui2
Muhammad ZainuriDiponegoro University2
Mada Triandala SiberoAfiliasi tidak diketahui2
A Suhaeli FahmiAfiliasi tidak diketahui2
Diah Permata WijayantiAfiliasi tidak diketahui2
Nenik KholilahAfiliasi tidak diketahui2
Nining NursalimAfiliasi tidak diketahui2
Endra WijayaAfiliasi tidak diketahui1
Nina RosidaAfiliasi tidak diketahui1
AmbariyantoAfiliasi tidak diketahui1
Delianis PringgeniesDiponegoro University1
Dear Berliana PutriAfiliasi tidak diketahui1
Syukur SyukurUniversitas Halu Oleo1
Zhulian Hikmah HasibuanAfiliasi tidak diketahui1
Diah Ayu Satyari UtamiAfiliasi tidak diketahui1
Sarjito SarjitoAfiliasi tidak diketahui1
Murni KarimAfiliasi tidak diketahui1
Muggi BachtiarAfiliasi tidak diketahui1
Umbu Domu WoraAfiliasi tidak diketahui1
Fanny Iriany GinzelAfiliasi tidak diketahui1
Eduard Meirenno TielmanAfiliasi tidak diketahui1
Eko Nurcahya DewiAfiliasi tidak diketahui1
Hafni RahmawatiAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://ejournal.undip.ac.id/index.php/ijms/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">The Potential of Cytotoxin and Antiviral in Sargassum polycystum and Sargassum ilicifolium’s  Polysaccharides Extract</dc:title>
	<dc:creator>Ningsih, Dwi Lestari Widya</dc:creator>
	<dc:creator>Trianto, Agus</dc:creator>
	<dc:creator>Widowati, Ita</dc:creator>
	<dc:creator>Magdugo, Rexie</dc:creator>
	<dc:creator>Hurtado, Anicia</dc:creator>
	<dc:creator>Marty, Christel</dc:creator>
	<dc:creator>Bourgougnon, Nathalie</dc:creator>
	<dc:subject xml:lang="en-US">bioactivity; seaweed; Sargassum sp; Herpes Simplex Virus (HSV)</dc:subject>
	<dc:description xml:lang="en-US">Marine algae known as one producers of bioactive compounds.  This study aims to analyze the cytotoxicity and antiviral activity in Sargassum polycystum and Sargassum ilicifolium tested with Herpes Simplex Virus (HSV).   The polysaccharides extract of algae was used in this study, as sulfated polysaccharides have been reported has bioactivity.  Cytotoxicity either antiviral could be correlated with the sulfate content as well as nature and chemical composition of the polysaccharides. Cytotoxicity and antiviral analysis based upon cell viability. Using the Vero cell / HSV-1 model, cytotoxicity was evaluated by incubating cellular suspensions (3.5×105 cells.mL-1) with various dilutions (concentration from 1 to 500 µg.mL-1, four wells per concentration) of fractions in 96-well plates (72h, 37°C, 5% CO2) in Eagle's MEM containing 8% FCS.  The cells were examined daily under a phase-contrast microscope to determine the minimum concentration of hydrolysate dry matter that induced alterations in cell morphology, including swelling, shrinkage, granularity and detachment. Algae S. illicifolium was found to have the highest cytotoxic content in each solution compared to S. polycystum. Algae S. illicifolium in KOH 4M (cellulose) reached 2,707 µg.ml-1, then HCl pH 2 (fucoidan) was 2,477 µg.ml-1, then CaCl2 2% (fucoidan) was 2,362 µg.ml-1, and in Na2CO3 3% (alginates) was 2,134 µg.ml-1. For antiviral, S. polycystum contained the highest antiviral compounds compared to S. illicifolium with KOH 4M (cellulose) solution was reached 67.02 µg.ml-1.  Then in Na2CO3 3% (alginates) which was 33.25 µg.ml-1, then CaCl2 2% (fucoidan) which was 31.62 µg.ml-1,and HCl pH 2 (fucoidan) was 30.08 µg.ml-1.  After all, the highest bioactivity compounds was found with KOH 4M (cellulose) for  cytotoxicity in S. ilicifolium and antiviral activity in S. polycystum. </dc:description>
	<dc:publisher xml:lang="en-US">Marine Science Department Diponegoro University</dc:publisher>
	<dc:date>2020-09-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ejournal.undip.ac.id/index.php/ijms/article/view/29964</dc:identifier>
	<dc:identifier>10.14710/ik.ijms.25.3.91-96</dc:identifier>
	<dc:source xml:lang="en-US">ILMU KELAUTAN: Indonesian Journal of Marine Sciences; Vol 25, No 3 (2020): Ilmu Kelautan; 91-96</dc:source>
	<dc:source>2406-7598</dc:source>
	<dc:source>0853-7291</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.undip.ac.id/index.php/ijms/article/view/29964/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 ILMU KELAUTAN: Indonesian Journal of Marine Sciences</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>