Producing Active Secondary Metabolite Against Pathogenic Vibrio spp. by Actinobacteria-Sodium Alginate Co-Culture

Azhar, Nuril; Yudiati, Ervia; Subagiyo, Subagiyo; Alghazeer, Rabia

doi:10.14710/ik.ijms.26.4.254-264

Akses terbuka Jurnal SINTA 1

Producing Active Secondary Metabolite Against Pathogenic Vibrio spp. by Actinobacteria-Sodium Alginate Co-Culture

ILMU KELAUTAN: Indonesian Journal of Marine Sciences · Vol. 26 No. 4 (2021) · hlm. 254–264

DOI: 10.14710/ik.ijms.26.4.254-264
ISSN: 2406-7598, 0853-7291
Terbit: 2021-12-06
Penerbit: Marine Science Department Diponegoro University
Bahasa: eng
Lisensi: creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi have been found in aquatic environments and suspected as the primary trigger of WFD (White Feces Disease) outbreaks in aquaculture. This Vibrio spp. has an antibiotic resistance to Ampicillin, Co-Amoxiclav, Amoxicillin, Azithromycin, Actinobacteria and Ciprofloxacin HCL. Actinobacteria and alginate have been reported to increase the marine biota resistance against diseases through prebiotic and probiotic mechanisms. This study aims to discover and increase the secondary metabolite production of Actinobacteria-Alginate and its ability as anti-vibrio. Alginate extraction in the samples dated September 2020 originally from Teluk Awur Bays, Jepara, Central Java, Indonesia (33.73±1.84%) was considerably higher than in May 2021 (22.67±0.3%). Samples were taken from sediment and mangrove root. Actinobacteria strains are macroscopically and microscopically similar to the genus Streptomyces. The most well-known antibiotics were produced by Streptomyces spp. The anti-vibrio test was carried out by Kirby-Bauer disc diffusion. The results were observed by measuring the inhibition zone surrounding the paper disc using a digital calliper. Co-culture strain 90 together with alginate have an approved antibacterial activity against all Vibrio spp. in the concentration of 10.disc-1 mg and 5 mg.disc-1. Co-culture Actinobacteria with alginate has remarkably changed the green-yellow color to olive green/dark red-orange (strains 3, 62, 63, 72, and 90), indicating the transformation of the formation alginate with pigments into other compounds through the biosynthetic pathway. Therefore, alginate enables to support of Actinobacteria by induction the active secondary metabolite as an anti-vibrio to counteract the bacterial pathogen diseases.

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Diponegoro University
Universitas Halu Oleo
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Rekan penulis	Organisasi	Artikel bersama
Agus Trianto	Afiliasi tidak diketahui	6
Nenik Kholilah	Afiliasi tidak diketahui	4
Wilis Ari Setyati	Afiliasi tidak diketahui	4
Mostafa Imhmed Ighwerb	Afiliasi tidak diketahui	4
Jelita Rahma Hidayati	Afiliasi tidak diketahui	4
Sri Sedjati	Afiliasi tidak diketahui	3
Diah Ayu Satyari Utami	Afiliasi tidak diketahui	3
Retno Hartati	Afiliasi tidak diketahui	3
Ali Djunaedi	Afiliasi tidak diketahui	3
Delianis Pringgenies	Diponegoro University	2
Endang Sri Susilo	Afiliasi tidak diketahui	2
Suryono Suryono	Afiliasi tidak diketahui	2
Ita Widowati	Afiliasi tidak diketahui	2
Wilis A Setyati	Afiliasi tidak diketahui	2
Ali Ridlo	Afiliasi tidak diketahui	2
JOHANNES HUTABARAT	Afiliasi tidak diketahui	2
Rudhi Pribadi	Afiliasi tidak diketahui	2
Ria Azizah	Afiliasi tidak diketahui	2
Norma Afiati	Afiliasi tidak diketahui	2
Muhammad Syaifudien Bahry	Diponegoro University	2
Ambariyanto	Afiliasi tidak diketahui	2
Rini Widyadmi	Afiliasi tidak diketahui	1
Amelia Cahya Anggelina	Diponegoro University	1
Ria Azizah Tri Nuraeni	Afiliasi tidak diketahui	1
Sunar Sunar	Afiliasi tidak diketahui	1
Diah Permata Wijayanti	Afiliasi tidak diketahui	1
Jasiel Junior Karosekali	Afiliasi tidak diketahui	1
Almay Atsiil Harits Syam	Afiliasi tidak diketahui	1
Muggi Bachtiar	Afiliasi tidak diketahui	1
Sutrisno Anggoro	Diponegoro University	1
Husna El Iksiroh	Afiliasi tidak diketahui	1
Henna Rya Abdurachim	Afiliasi tidak diketahui	1
Syukur Syukur	Universitas Halu Oleo	1
Zhulian Hikmah Hasibuan	Afiliasi tidak diketahui	1
Edi Wibowo	Afiliasi tidak diketahui	1
Tika Retnowati	Afiliasi tidak diketahui	1

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Sumber metadata: OAI-PMH (metadata jurnal) http://ejournal.undip.ac.id/index.php/ijms/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.

Metadata asli (oai_dc)

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	<dc:title xml:lang="en-US">Producing Active Secondary Metabolite Against Pathogenic Vibrio spp. by Actinobacteria-Sodium Alginate Co-Culture</dc:title>
	<dc:creator>Azhar, Nuril</dc:creator>
	<dc:creator>Yudiati, Ervia</dc:creator>
	<dc:creator>Subagiyo, Subagiyo</dc:creator>
	<dc:creator>Alghazeer, Rabia</dc:creator>
	<dc:subject xml:lang="en-US">Alginate; Actinobacteria; Co-culture; Vibrio spp.</dc:subject>
	<dc:description xml:lang="en-US">Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi have been found in aquatic environments and suspected as the primary trigger of WFD (White Feces Disease) outbreaks in aquaculture. This Vibrio spp. has an antibiotic resistance to Ampicillin, Co-Amoxiclav, Amoxicillin, Azithromycin, Actinobacteria and Ciprofloxacin HCL. Actinobacteria and alginate have been reported to increase the marine biota resistance against diseases through prebiotic and probiotic mechanisms. This study aims to discover and increase the secondary metabolite production of Actinobacteria-Alginate and its ability as anti-vibrio. Alginate extraction in the samples dated September 2020 originally from Teluk Awur Bays, Jepara, Central Java, Indonesia (33.73±1.84%) was considerably higher than in May 2021 (22.67±0.3%). Samples were taken from sediment and mangrove root. Actinobacteria strains are macroscopically and microscopically similar to the genus Streptomyces. The most well-known antibiotics were produced by Streptomyces spp. The anti-vibrio test was carried out by Kirby-Bauer disc diffusion. The results were observed by measuring the inhibition zone surrounding the paper disc using a digital calliper. Co-culture strain 90 together with alginate have an approved antibacterial activity against all Vibrio spp. in the concentration of 10.disc-1 mg and 5 mg.disc-1. Co-culture Actinobacteria with alginate has remarkably changed the green-yellow color to olive green/dark red-orange (strains 3, 62, 63, 72, and 90), indicating the transformation of the formation alginate with pigments into other compounds through the biosynthetic pathway. Therefore, alginate enables to support of Actinobacteria by induction the active secondary metabolite as an anti-vibrio to counteract the bacterial pathogen diseases.</dc:description>
	<dc:publisher xml:lang="en-US">Marine Science Department Diponegoro University</dc:publisher>
	<dc:date>2021-12-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
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	<dc:identifier>https://ejournal.undip.ac.id/index.php/ijms/article/view/41215</dc:identifier>
	<dc:identifier>10.14710/ik.ijms.26.4.254-264</dc:identifier>
	<dc:source xml:lang="en-US">ILMU KELAUTAN: Indonesian Journal of Marine Sciences; Vol 26, No 4 (2021): Ilmu Kelautan; 254-264</dc:source>
	<dc:source>2406-7598</dc:source>
	<dc:source>0853-7291</dc:source>
	<dc:language>eng</dc:language>
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	<dc:relation>https://ejournal.undip.ac.id/index.php/ijms/article/downloadSuppFile/41215/9172</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 ILMU KELAUTAN: Indonesian Journal of Marine Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
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