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Pigments Characterization and Molecular Identification of Bacterial Symbionts of Brown Algae Padinasp. Collected from Karimunjawa Island

ILMU KELAUTAN: Indonesian Journal of Marine Sciences · Vol. 21 No. 2 (2016) · hlm. 58–64

DOI
10.14710/ik.ijms.21.2.58-64
ISSN
2406-7598, 0853-7291
Terbit
2016-06-01
Penerbit
Marine Science Department Diponegoro University
Bahasa
eng

Abstrak

The search for carotenoids in nature has been extensively studied because of their applications in foods. One treasure of the biopigment source is symbiotic-microorganisms with marine biota. The advantages of symbiont bacteria are easy to culture and sensitize pigments. The use of symbiont bacteria helps to conserve fish, coral reefs, seagrass, and seaweed. Therefore, the bacteria keeps their existence in their ecosystems. In this study, bacterial symbionts were successfully isolated from brown algae Padina sp. The bacterial symbionts had yellow pigment associated with carotenoids. The pigments were characterized using High Performance Liquid Chromatography (HPLC) with a Photo Diode Array (PDA) detector. The carotenoid pigments in the bacterial symbionts were identified as dinoxanthin, lutein and neoxanthin. Molecular identification by using a 16S rRNA gene sequence method, reveals that the bacterial symbionts were closely related to Bacillus marisflavi with a homology of 99%. Keywords:carotenoid pigments, brown algae, Padina, bacterial symbionts, 16S rRNA

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  • Diponegoro University
  • State University of Jakarta
  • Satya Wacana Christian University
  • Yamaguchi University
  • Universitas Ma Chung
  • Politeknik Negeri Cilacap
  • Syarif Hidayatullah State Islamic University Jakarta
  • Organisasi lain
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Rekan penulisOrganisasiArtikel bersama
Agus SabdonoDiponegoro University7
Agus TriantoDiponegoro University6
Vivi Endar HerawatiDiponegoro University3
Johannes HutabaratDiponegoro University3
Ferry Fredy KarwurSatya Wacana Christian University3
Slamet Budi PrayitnoDiponegoro University2
Tonny BachtiarDiponegoro University2
Khoeruddin WittriansyahPoliteknik Negeri Cilacap2
Mada Triandala SiberoDiponegoro University2
TAKENORI NITTAYamaguchi University1
Rini PuspitaningrumState University of Jakarta1
Amien Ramadhan IshakState University of Jakarta1
Risma Dwi UtariState University of Jakarta1
Mella FeraniaState University of Jakarta1
Chris AdhiyantoSyarif Hidayatullah State Islamic University Jakarta1
Hattori YukioYamaguchi University1
Yasuhiro YamashiroYamaguchi University1
. PinandoyoAfiliasi tidak diketahui1
Pinandoyo PinandoyoDiponegoro University1
Seto WindartoDiponegoro University1
Putut HariyadiDiponegoro University1
YS DarmantoDiponegoro University1
Nurmanita RismaningsihNagoya University1
Edi SuhartonoDiponegoro University1
Uswatun KhasanahDiponegoro University1
Agus HardiyantoDiponegoro University1
Tri Yuni AtmojoDiponegoro University1
Tony BachtiarDiponegoro University1
Budi PrasetyoState University of Semarang1
Helly de FretesSatya Wacana Christian University1
Heriyanto HeriyantoUniversitas Ma Chung1
Tatas Hardo Panintingjati BrotosudarmoUniversitas Ma Chung1
Leenawaty LimantaraUniversitas Ma Chung1
Sarjito SarjitoDiponegoro University1
Sahala HutabaratDiponegoro University1
Dian Ridwan NurdianaNational Research and Innovation Agency1

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Sumber metadata: OAI-PMH (metadata jurnal) http://ejournal.undip.ac.id/index.php/ijms/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
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	<dc:title xml:lang="en-US">Pigments Characterization and Molecular Identification of Bacterial Symbionts of Brown Algae Padinasp. Collected from Karimunjawa Island</dc:title>
	<dc:creator>Murti, Damar Bayu</dc:creator>
	<dc:creator>Susanto, A B</dc:creator>
	<dc:creator>Radjasa, Ocky Karna</dc:creator>
	<dc:creator>Rondonuwu, Ferdy Semuel</dc:creator>
	<dc:description xml:lang="en-US">The search for carotenoids in nature has been extensively studied because of their applications in foods. One treasure of the biopigment source is symbiotic-microorganisms with marine biota. The advantages of symbiont bacteria are easy to culture and sensitize pigments. The use of symbiont bacteria helps to conserve fish, coral reefs, seagrass, and seaweed. Therefore, the bacteria keeps their existence in their ecosystems. In this study, bacterial symbionts were successfully isolated from brown algae Padina sp. The bacterial symbionts had yellow pigment associated with carotenoids. The pigments were characterized using High Performance Liquid Chromatography (HPLC) with a Photo Diode Array (PDA) detector. The carotenoid pigments in the bacterial symbionts were identified as dinoxanthin, lutein and neoxanthin. Molecular identification by using a 16S rRNA gene sequence method, reveals that the bacterial symbionts were closely related to Bacillus marisflavi with a homology of 99%.    Keywords :carotenoid pigments, brown algae, Padina, bacterial symbionts, 16S rRNA</dc:description>
	<dc:publisher xml:lang="en-US">Marine Science Department Diponegoro University</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2016-06-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
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	<dc:identifier>https://ejournal.undip.ac.id/index.php/ijms/article/view/11379</dc:identifier>
	<dc:identifier>10.14710/ik.ijms.21.2.58-64</dc:identifier>
	<dc:source xml:lang="en-US">ILMU KELAUTAN: Indonesian Journal of Marine Sciences; Vol 21, No 2 (2016): Ilmu Kelautan; 58-64</dc:source>
	<dc:source>2406-7598</dc:source>
	<dc:source>0853-7291</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.undip.ac.id/index.php/ijms/article/view/11379/pdf</dc:relation>
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