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Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent

ILMU KELAUTAN: Indonesian Journal of Marine Sciences · Vol. 28 No. 2 (2023) · hlm. 136–147

DOI
10.14710/ik.ijms.28.2.136-147
ISSN
2406-7598, 0853-7291
Terbit
2023-05-21
Penerbit
Marine Science Department Diponegoro University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Mangroves are complex, unique, and dynamic ecosystems that host organisms that have special physiological adaptations to fluctuations in salinity, temperature, and pH. Gastropods have an important role in the mangrove ecosystem for food cycles and webs by helping to provide nutrients and micro-habitats for microbes. Micro-fungi isolated from mangrove ecosystems are productive sources of enzymes and bioactive compounds such as antibacterial and antifungal. In this study a explored the enzymatic and anti-vibrio activity of fungi associated with the marine gastropod Cerithidea cingulata and Lottia scabra. collected in three mangrove forests in North Sulawesi, Indonesia. The fungi associated with the specimens were inoculated on malt extract agar (MEA) using the tapping method. Enzymatic and anti-vibrio assays were conducted applying the plug method. Positive effects of the fungal compounds on Vibrio were indicated by clear zones formed around the isolates. A total of 13 fungal species were isolated from C. cingulata (19MB-C5 and 19MBa-A4) and L. scabra (19MT-07 and 19MT-18). Most fungal isolates have moderate growth rate. The isolate with highest cellulolytic, proteolytic, and anti-vibriosis activity (Vibrio parahaemolyticus, V. harveyi, V. vulnificus, and V. alginolyticus), derived from C. cingulata 19MB-C5 was closely related to Aspergillus niger, as revealed by molecular analysis using the universal primers ITS1 and ITS4 (similarity value 99%). A. niger 19MB-C5-3 has potential as a bioremediation and anti-vibrio agent that can be applied in aquaculture.

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  • Diponegoro University
  • State University of Semarang
  • Tadulako University
  • Edinburgh Napier University
  • Satya Wacana Christian University
  • Politeknik Negeri Cilacap
  • Sekolah Tinggi Teknologi Migas
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  • Afiliasi tidak diketahui
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Agus SabdonoDiponegoro University12
Wilis Ari SetyatiDiponegoro University5
Khoeruddin WittriansyahPoliteknik Negeri Cilacap4
Mada Triandala SiberoDiponegoro University4
Vivi Endar HerawatiDiponegoro University3
Johannes HutabaratDiponegoro University3
Norma AfiatiDiponegoro University3
Ervia YudiatiDiponegoro University3
Slamet Budi PrayitnoDiponegoro University2
Tonny BachtiarDiponegoro University2
AB SusantoDiponegoro University2
Nenik KholilahDiponegoro University2
Ferry Fredy KarwurSatya Wacana Christian University2
Ambariyanto AmbariyantoDiponegoro University2
Retno MurwaniDiponegoro University2
Delianis PringgeniesDiponegoro University2
Nuril AzharDiponegoro University2
Dwi Haryo IsmunartiDiponegoro University2
Diah Permata WijayantiDiponegoro University2
Trida Ridho FarizState University of Semarang1
Purwanto PurwantoDiponegoro University1
Andhina Putri HeriyantiState University of Semarang1
B. SulaimanDiponegoro University1
Azis Nur BambangDiponegoro University1
Mohammad LutfiSekolah Tinggi Teknologi Migas1
Ebtihal Modather MohammedOmdurman Islamic University1
Rini WidyadmiAfiliasi tidak diketahui1
Amelia Cahya AnggelinaDiponegoro University1
Popi IL AyerAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://ejournal.undip.ac.id/index.php/ijms/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent</dc:title>
	<dc:creator>Trianto, Agus</dc:creator>
	<dc:creator>Radjasa, Ocky Karna</dc:creator>
	<dc:creator>Subagiyo, Subagiyo</dc:creator>
	<dc:creator>Isabella, Armeilia Ardiva</dc:creator>
	<dc:creator>Bahry, Muhammad Syaifudien</dc:creator>
	<dc:creator>Purnaweni, Hartuti</dc:creator>
	<dc:creator>Djamaludin, Rignolda</dc:creator>
	<dc:creator>Tjoa, Aiyen</dc:creator>
	<dc:creator>Singleton, Ian</dc:creator>
	<dc:creator>Evans, Darren Mark</dc:creator>
	<dc:creator>Diele, Karen</dc:creator>
	<dc:subject xml:lang="en-US">enzyme; fungi; mangrove; vibriosis</dc:subject>
	<dc:description xml:lang="en-US">Mangroves are complex, unique, and dynamic ecosystems that host organisms that have special physiological adaptations to fluctuations in salinity, temperature, and pH. Gastropods have an important role in the mangrove ecosystem for food cycles and webs by helping to provide nutrients and micro-habitats for microbes.  Micro-fungi isolated from mangrove ecosystems are productive sources of enzymes and bioactive compounds such as antibacterial and antifungal. In this study a explored the enzymatic and anti-vibrio activity of fungi associated with the marine gastropod Cerithidea cingulata and Lottia scabra. collected in three mangrove forests in North Sulawesi, Indonesia.  The fungi associated with the specimens were inoculated on malt extract agar (MEA) using the tapping method. Enzymatic and anti-vibrio assays were conducted applying the plug method. Positive effects of the fungal compounds on Vibrio  were indicated by  clear zones formed around the isolates.  A total of 13 fungal species were isolated from C. cingulata (19MB-C5  and 19MBa-A4) and L. scabra (19MT-07 and 19MT-18).  Most fungal isolates have moderate growth rate. The isolate with highest cellulolytic, proteolytic, and anti-vibriosis activity (Vibrio parahaemolyticus, V. harveyi, V. vulnificus, and V. alginolyticus), derived from C. cingulata 19MB-C5 was closely related to Aspergillus niger, as revealed by molecular analysis using the universal primers ITS1 and ITS4 (similarity value 99%).  A. niger 19MB-C5-3 has potential as a bioremediation and anti-vibrio agent that can be applied in aquaculture.</dc:description>
	<dc:publisher xml:lang="en-US">Marine Science Department Diponegoro University</dc:publisher>
	<dc:date>2023-05-21</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
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	<dc:identifier>https://ejournal.undip.ac.id/index.php/ijms/article/view/46390</dc:identifier>
	<dc:identifier>10.14710/ik.ijms.28.2.136-147</dc:identifier>
	<dc:source xml:lang="en-US">ILMU KELAUTAN: Indonesian Journal of Marine Sciences; Vol 28, No 2 (2023): Ilmu Kelautan; 136-147</dc:source>
	<dc:source>2406-7598</dc:source>
	<dc:source>0853-7291</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.undip.ac.id/index.php/ijms/article/view/46390/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 ILMU KELAUTAN: Indonesian Journal of Marine Sciences</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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