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Analysis of Carotenoids and the Identification of Mangrove Sediment Bacteria of Segara Anakan, Cilacap

ILMU KELAUTAN: Indonesian Journal of Marine Sciences · Vol. 23 No. 4 (2019) · hlm. 163–170

DOI
10.14710/ik.ijms.23.4.163-170
ISSN
2406-7598, 0853-7291
Terbit
2019-01-04
Penerbit
Marine Science Department Diponegoro University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The excessive consumption of artificial dyes can lead to negative effects on human body. Thus, the invention of natural dyes, such as carotenoids, is needed in order to reduce the negative impacts. Carotenoids are yellow, orange, and reddish yellow pigments produced by plants, animals, algae, and microorganisms. This study was aimed to analyze the carotenoid pigments of mangrove sediment bacteria in Segara Anakan, Cilacap, and to identify species of bacteria that can produce carotenoids. Carotenoids were analysed by using Thin Layer Chromatography (TLC) and UV-Vis spectrophotometer. Meanwhile molecular identification of bacteria was carried by 16S rDNA PCR and DNA sequence was analysed through a program called Basic Local Alignment Search Tool (BLAST). The kinship of bacteria was shown in Phylogenetic tree by using Bioedit and MEGA 5 software. Qualitative analysis by using TLC produced several pigments like: β-carotene, β-isorenieraten, lycopene, flavonoids, chlorophyll a, chlorophyll b and a feofitin with Rf value of 0.36-0.95. Quantitative analysis showed that bacteria KH (greenish yellow), KT (dark yellow) and KM (light yellow) produced carotenoids at 95.30 mg.g-1, 110.34 mg.g-1 and 25.349 mg.g-1. KH, KT and KM were suspected to be bacteria known as Streptomyces chartreusis, Bacillus megaterium and Streptomyces chromofuscus with similarity of 99%. Mangrove sediment bacteria had the potential of producing carotenoids as an alternative of eco-friendly natural dyes.

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TONNY BACHTIARAfiliasi tidak diketahui3
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Sumber metadata: OAI-PMH (metadata jurnal) http://ejournal.undip.ac.id/index.php/ijms/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Analysis of Carotenoids and the Identification of Mangrove Sediment Bacteria of Segara Anakan, Cilacap</dc:title>
	<dc:creator>Riyanti, Riyanti</dc:creator>
	<dc:creator>Rahmawati, Meris</dc:creator>
	<dc:creator>Hidayati, Nuning Vita</dc:creator>
	<dc:creator>Syakti, Agung Dhamar</dc:creator>
	<dc:creator>Radjasa, Ocky Karna</dc:creator>
	<dc:subject xml:lang="en-US">pigment; Streptomyces; Bacillus; natural dyes</dc:subject>
	<dc:description xml:lang="en-US">The excessive consumption of artificial dyes can lead to negative effects on human body. Thus, the invention of natural dyes, such as carotenoids, is needed in order to reduce the negative impacts. Carotenoids are yellow, orange, and reddish yellow pigments produced by plants, animals, algae, and microorganisms. This study was aimed to analyze the carotenoid pigments of mangrove sediment bacteria in Segara Anakan, Cilacap, and to identify species of bacteria that can produce carotenoids. Carotenoids were analysed by using Thin Layer Chromatography (TLC) and UV-Vis spectrophotometer. Meanwhile molecular identification of bacteria was carried by 16S rDNA PCR and DNA sequence was analysed through a program called Basic Local Alignment Search Tool (BLAST). The kinship of bacteria was shown in Phylogenetic tree by using Bioedit and MEGA 5 software. Qualitative analysis by using TLC produced several pigments like: β-carotene, β-isorenieraten, lycopene, flavonoids, chlorophyll a, chlorophyll b and a feofitin with Rf value of 0.36-0.95. Quantitative analysis showed that bacteria KH (greenish yellow), KT (dark yellow) and KM (light yellow) produced carotenoids at 95.30 mg.g-1, 110.34 mg.g-1 and 25.349 mg.g-1. KH, KT and KM were suspected to be bacteria known as Streptomyces chartreusis, Bacillus megaterium and Streptomyces chromofuscus with similarity of 99%. Mangrove sediment bacteria had the potential of producing carotenoids as an alternative of eco-friendly natural dyes.</dc:description>
	<dc:publisher xml:lang="en-US">Marine Science Department Diponegoro University</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2019-01-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ejournal.undip.ac.id/index.php/ijms/article/view/15266</dc:identifier>
	<dc:identifier>10.14710/ik.ijms.23.4.163-170</dc:identifier>
	<dc:source xml:lang="en-US">ILMU KELAUTAN: Indonesian Journal of Marine Sciences; Vol 23, No 4 (2018): Ilmu Kelautan; 163-170</dc:source>
	<dc:source>2406-7598</dc:source>
	<dc:source>0853-7291</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.undip.ac.id/index.php/ijms/article/view/15266/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 ILMU KELAUTAN: Indonesian Journal of Marine Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>