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Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells

Indonesian Journal of Biotechnology · Vol. 10 No. 2 (2005) · hlm. 822–829

DOI
10.22146/ijbiotech.7553
ISSN
2089-2241, 0853-8654
Terbit
2005-12-15
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effects on the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somatic cells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved and serum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclear envelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. There was no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicating that either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasm environments in similar pattern. Although the fusion rate was not significantly different among the groups, the proportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%) was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos that developed to the morula and blastocyst stages were not significantly different among the groups. Results of these studies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluent somatic cells, showed similar developmental competence to the blastocyst stage.

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  • IPB University
  • National Research and Innovation Agency
  • Yamaguchi University
  • Institute of Agrobiological Sciences
  • Universitas Gadjah Mada
  • Kementerian Kesehatan Republik Indonesia
  • Sekolah Tinggi Agama Kristen Protestan Negeri Sentani
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Rekan penulisOrganisasiArtikel bersama
Raden Iis ArifiantiniIPB University8
Wahono Esthi PrasetyaningtyasIPB University6
Mohamad Agus SetiadiIPB University5
Kazuhiro KikuchiInstitute of Agrobiological Sciences4
Arief BoedionoIPB University3
Bambang PurwantaraIPB University3
Ekayanti Mulyawati KaiinNational Research and Innovation Agency3
I Ketut Mudite AdnyaneIPB University3
Vista BudiariatiUniversitas Gadjah Mada2
Dwi BudionoIPB University2
Mohamad Agus SetiadiIPB University2
Yusuf TLAfiliasi tidak diketahui2
Tatan KostamanNational Research and Innovation Agency2
Setiadi MaAfiliasi tidak diketahui2
Faisal Amri SatrioIPB University2
Aisyah Fidela SiregarIPB University2
Rizal GusdinarIPB University2
Muhammad ElmanavieanSekolah Tinggi Agama Kristen Protestan Negeri Sentani2
Arifin Budiman NugrahaIPB University2
Berry JuliandiIPB University1
Ratih RinendyaputriKementerian Kesehatan Republik Indonesia1
Feni Dwi Kartika GuloIPB University1
Cece SumantriIPB University1
Al Mukhlas FikriIPB University1
Noer Muhammad Dliyaul HaqIPB University1
Diah Nugrahani PristihadiIPB University1
Hastuti HastutiIPB University1
Maghfira MaghfiraTadulako University1
Tike SartikaNational Research and Innovation Agency1
Ekayanti Muyawati KaiinAfiliasi tidak diketahui1
Frilianty PutriIPB University1
A.R. SetiokoAfiliasi tidak diketahui1
Brilla Widya WitriIPB University1
Nurhidayat NurhidayatIPB University1
Kusdiantoro MohamadIPB University1
Adrian SitumeangUniversity of Indonesia1
Handina RakhmawatiIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells</dc:title>
	<dc:creator>Fahrudin, Mokhamad</dc:creator>
	<dc:creator>Karja, Ni Wayan Kurniani</dc:creator>
	<dc:creator>Suzuki, Tatsuyuki</dc:creator>
	<dc:subject xml:lang="en-US">nuclear transplantation technique-somatic cells-nucleus swelling</dc:subject>
	<dc:description xml:lang="en-US">This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effects on the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somatic cells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved and serum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclear envelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. There was no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicating that either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasm environments in similar pattern. Although the fusion rate was not significantly different among the groups, the proportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%) was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos that developed to the morula and blastocyst stages were not significantly different among the groups. Results of these studies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluent somatic cells, showed similar developmental competence to the blastocyst stage.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2005-12-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7553</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7553</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 10, No 2 (2005); 822-829</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7553/5873</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>