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Detection of eae, bfpA, espA Genes on Diarrhoeagenic Strains of Escherichia coli Isolates

Indonesian Journal of Biotechnology · Vol. 11 No. 1 (2006) · hlm. 889–893

DOI
10.22146/ijbiotech.7563
ISSN
2089-2241, 0853-8654
Terbit
2006-06-01
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

The Enteropathogenic Escherichia coli (EPEC) is one of pathogenic strain of diarrheagenic E. coli group in children and infant that occurs in developing countries. The significant virulence factors in pathogenic EPEC are eaeA (E. coli attaching- effacing ), bfpA (bundle-forming pilus A) and espA (encoding secreted protein A) genes. The use of DNA probes to detect the virulence genes in E. coli in Indonesia is not common yet. In this experiment the gene fragments of eae, bfpA, and espA were used as probes to detect the EPEC among E. coli isolates from stool specimensin of diarrheic children attending Public Health Centers in Yogyakarta. The DNA samples were isolated from 49 diarrheagenic E. coli isolates. The DNA probes of eae, bfpA and espA were obtained by amplification of DNA fragment of EPEC O126 using PCR technique. Furthermore, those probes were used to identify the presence of those genes among E. coli isolates using hybridization technique. The results showed that 42 (85.7%) isolates were espA+, 25 isolates (51%) were eaeA+ (EPEC strains). Therefore among 25 isolates of EPEC, 20 isolates (80 %) among EPEC were bfpA+ (typical EPEC strains).

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  • Universitas Gadjah Mada
  • Sebelas Maret University
  • IPB University
  • Muhammadiyah University of Yogyakarta
  • Universitas Airlangga
  • Sriwijaya University
  • Riau University
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Rekan penulisOrganisasiArtikel bersama
Michael Haryadi WibowoUniversitas Gadjah Mada4
Wayan Tunas ArtamaUniversitas Gadjah Mada4
Arum SetiawanSriwijaya University2
Mammed SagiUniversitas Gadjah Mada2
Istriyati IstriyatiUniversitas Gadjah Mada2
Marsetyawan H. N. E. SoesatyoUniversitas Gadjah Mada2
Triyanto TriyantoUniversitas Gadjah Mada2
Nursyirwani NursyirwaniRiau University2
A. E. T. H. WahyuniUniversitas Gadjah Mada2
Jaka WidadaUniversitas Gadjah Mada2
Alimuddin AlimuddinIPB University2
Sebastian MarginoUniversitas Gadjah Mada2
Widodo WidodoIPB University2
Sri DarmawatiUniversitas Muhammadiyah Semarang2
Langkah SembiringUniversitas Gadjah Mada2
Tri UntariAfiliasi tidak diketahui2
Ririn Teguh ArdiansyahUniversitas Mandala Waluya1
Damar Tri BoewonoAfiliasi tidak diketahui1
Pinandi Sri PudyaniUniversitas Gadjah Mada1
Ika Dewi AnaUniversitas Gadjah Mada1
Tita Ratya UtariMuhammadiyah University of Yogyakarta1
Siti SunarintyasUniversitas Gadjah Mada1
Juni HandajaniUniversitas Gadjah Mada1
Yulita KristantiUniversitas Gadjah Mada1
Risya CilmiatySebelas Maret University1
Mandojo RukmoUniversitas Airlangga1
Afiono Agung PrasetyoSebelas Maret University1
Khilyat Ulin Nur ZainiSebelas Maret University1
Suhartono Taat PutraUniversitas Airlangga1
Banun KusumawardaniUniversitas Jember1
Djaswadi DasukiUniversitas Gadjah Mada1
Al Supartinah SantosoUniversitas Gadjah Mada1
Regina TC. TandelilinUniversitas Gadjah Mada1
Abdul Salam M. SofroYarsi University1
Widjijono WidjijonoUniversitas Gadjah Mada1
Nuryono NuryonoUniversitas Gadjah Mada1
Dwi Aji NugrohoMuhammadiyah University of Yogyakarta1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Detection of eae, bfpA, espA Genes on Diarrhoeagenic Strains of Escherichia coli Isolates</dc:title>
	<dc:creator>Harti, Agnes Sri</dc:creator>
	<dc:creator>Iravati, Susi</dc:creator>
	<dc:creator>Asmara, Widya</dc:creator>
	<dc:subject xml:lang="en-US">DNA probe, eae, bfpA, espA, EPEC</dc:subject>
	<dc:description xml:lang="en-US">The Enteropathogenic Escherichia coli (EPEC) is one of pathogenic strain of diarrheagenic E. coli group in children and infant that occurs in developing countries. The significant virulence factors in pathogenic EPEC are eaeA (E. coli attaching- effacing ), bfpA (bundle-forming pilus A) and espA (encoding secreted protein A) genes. The use of DNA probes to detect the virulence genes in E. coli in Indonesia is not common yet. In this experiment the gene fragments of eae, bfpA, and espA were used as probes to detect the EPEC among E. coli isolates from stool specimensin of diarrheic children attending Public Health Centers in Yogyakarta. The DNA samples were isolated from 49 diarrheagenic E. coli isolates. The DNA probes of eae, bfpA and espA were obtained by amplification of DNA fragment of EPEC O126 using PCR technique. Furthermore, those probes were used to identify the presence of those genes among E. coli isolates using hybridization technique. The results showed that 42 (85.7%) isolates were espA+,   25 isolates (51%) were eaeA+    (EPEC strains). Therefore among 25 isolates of EPEC, 20 isolates (80 %) among EPEC were bfpA+  (typical EPEC strains).</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2006-06-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7563</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7563</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 11, No 1 (2006); 889-893</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7563/5883</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>