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Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR

Indonesian Journal of Biotechnology · Vol. 11 No. 2 (2006)

DOI
10.22146/ijbiotech.7569
ISSN
2089-2241, 0853-8654
Terbit
2006-12-01
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detectingdengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primersthat conserve for several Flavivirus. The results showed that in 12 suspect patients, 100% were positive for Flavivirusand there are some genotypic variation among them, that indicated by several RT-PCR products higher than 511 bp,the expected product for RT-PCR. Further assay was performed to clarify the presence and serotypes of dengue virususing multiplex nested PCR. Serotyping results indicated that 83,3% of samples can be confirmed for dengue virus.Among the dengue virus positive 16,7 % are dengue-2, 16.7 % are dengue-3, and the most common 50% are dengue-4,whereas dengue-1 were not found among the patients. The combination of RT-PCR and multiplex nested PCR assaycan be used for rapid analysis dengue samples in early phase which is potentially useful for clinical, epidemiologyand also evolutionary studies.Key words: Flavivirus, dengue virus, serotype, RT-PCR, multiplex nested PCR

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Michael KannAfiliasi tidak diketahui7
Hevi WihadmadyatamiAfiliasi tidak diketahui4
Warih Pulung NugrahaniAfiliasi tidak diketahui3
Jaka WidadaUniversitas Gadjah Mada3
Krisna Noli AndrianAfiliasi tidak diketahui3
Farida J. RachmawatyAfiliasi tidak diketahui2
Marsetyawan H.N.E. SoesatyoAfiliasi tidak diketahui2
Diana SavitriAfiliasi tidak diketahui2
Irhamna PutriAfiliasi tidak diketahui2
Medania PurwaningrumAfiliasi tidak diketahui2
A. PurnomoadiAfiliasi tidak diketahui2
Nenny Sri MulyaniAfiliasi tidak diketahui2
Titis WidowatiAfiliasi tidak diketahui2
Purnomo HadiAfiliasi tidak diketahui2
Abdul Rahman SiregarAfiliasi tidak diketahui2
Abu Tholib AmanAfiliasi tidak diketahui2
Yati SoenartoAfiliasi tidak diketahui2
Diani MentariAfiliasi tidak diketahui1
Nastiti WijayantiUniversitas Gadjah Mada1
Rinawati RinawatiAfiliasi tidak diketahui1
E SuharyantoAfiliasi tidak diketahui1
Muhammad AdnanAfiliasi tidak diketahui1
Matin NuhamunadaAfiliasi tidak diketahui1
Lisna HidayatiAfiliasi tidak diketahui1
Apon Zaenal MustopaAfiliasi tidak diketahui1
Huda Salahudin DarusmanAfiliasi tidak diketahui1
Muh. Chaeril IkramullahAfiliasi tidak diketahui1
Ai HertatiAfiliasi tidak diketahui1
Rifqiyah Nur UmamiAfiliasi tidak diketahui1
Lita Tri RatnaAfiliasi tidak diketahui1
Shasmita IrawanAfiliasi tidak diketahui1
Moh Egy Rahman FirdausAfiliasi tidak diketahui1
Erni MartaniAfiliasi tidak diketahui1
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Camelia HerdiniAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR</dc:title>
	<dc:creator>Wijayanti, Nastiti</dc:creator>
	<dc:creator>Nirwati, Hera</dc:creator>
	<dc:creator>Wibawa, Tri</dc:creator>
	<dc:creator>Haryanto, Aris</dc:creator>
	<dc:creator>Sutaryo, S.</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detectingdengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primersthat conserve for several Flavivirus. The results showed that in 12 suspect patients, 100% were positive for Flavivirusand there are some genotypic variation among them, that indicated by several RT-PCR products higher than 511 bp,the expected product for RT-PCR. Further assay was performed to clarify the presence and serotypes of dengue virususing multiplex nested PCR. Serotyping results indicated that 83,3% of samples can be confirmed for dengue virus.Among the dengue virus positive 16,7 % are dengue-2, 16.7 % are dengue-3, and the most common 50% are dengue-4,whereas dengue-1 were not found among the patients. The combination of RT-PCR and multiplex nested PCR assaycan be used for rapid analysis dengue samples in early phase which is potentially useful for clinical, epidemiologyand also evolutionary studies.Key words: Flavivirus, dengue virus, serotype, RT-PCR, multiplex nested PCR</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2006-12-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7569</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7569</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 11, No 2 (2006)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7569/5889</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>