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T47D cells arrested at G2M and Hyperploidy Formation Induced by a Curcumin’s Analogue PGV-1

Indonesian Journal of Biotechnology · Vol. 12 No. 2 (2007)

DOI
10.22146/ijbiotech.7776
ISSN
2089-2241, 0853-8654
Terbit
2007-12-09
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

its chemical structure than curcumin. As a curcumin analogue, PGV-1 was considered to have anticanceractivities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cytotoxiceffects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progressionwas carried out using flowcytometry. Western blot analysis was used to analyze protein expression correspondingto cell cycle progression. The result showed that at the concentration of 2.5 μM PGV-1 inhibited cell cycleprogression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation inducedby PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 μM elevated the level of p21 CIP/KIPthrough p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated byPARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibitsthe growth of T47D cells targeted on microtubules.Keywords: PGV-1, G2/M arrest, apoptosis, p21

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  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Nara Institute of Science and Technology
  • Kementerian Kesehatan Republik Indonesia
  • Atma Jaya University Yogyakarta
  • Muhammadiyah University Purwokerto
  • Tanjungpura University
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Rekan penulisOrganisasiArtikel bersama
Ratna Asmah SusidartiUniversitas Gadjah Mada17
Adam HermawanUniversitas Gadjah Mada17
Riris Istighfari JenieUniversitas Gadjah Mada15
Muthi IkawatiUniversitas Gadjah Mada11
Rohmad Yudi UtomoUniversitas Gadjah Mada8
Dyaningtyas Dewi Pamungkas PutriUniversitas Gadjah Mada7
Faaza Aulia RahmanUniversitas Gadjah Mada5
Sari HaryantiKementerian Kesehatan Republik Indonesia5
Dhania NovitasariUniversitas Gadjah Mada4
Retno MurwantiUniversitas Gadjah Mada4
Desty Restia RahmawatiUniversitas Gadjah Mada4
Sri HandayaniIndonesian Institute of Sciences3
Ummi Maryam ZulfinUniversitas Gadjah Mada3
Anindyajati AnindyajatiUniversitas Gadjah Mada3
Larasati LarasatiUniversitas Gadjah Mada3
Erlina RivantiUniversitas Gadjah Mada3
Nunuk Aries NurulitaMuhammadiyah University Purwokerto3
M. MurwantokoAfiliasi tidak diketahui2
Sendy JunediAtma Jaya University Yogyakarta2
Langkah SembiringUniversitas Gadjah Mada2
Suwijiyo PramonoUniversitas Gadjah Mada2
Zalinar UdinIndonesian Institute of Sciences2
Yogi ErtantoUniversitas Gadjah Mada2
Lailatul QodriaUniversitas Gadjah Mada2
Gergorius Gena MaranUniversitas Gadjah Mada2
Aulia Nur SeptianiUniversitas Gadjah Mada2
Sofa FaridaUniversitas Gadjah Mada2
Beni LestariUniversitas Gadjah Mada2
Asri Mega PutriUniversitas Gadjah Mada2
Hilyatul FadliyahUniversitas Gadjah Mada2
Indri KusharyantiTanjungpura University2
Yonika Arum LarasatiUniversitas Gadjah Mada2
Raditya Prima IstiajiUniversitas Gadjah Mada2
Retno Sunarminingsih SudibyoUniversitas Gadjah Mada2
Sofia MubarikaUniversitas Gadjah Mada2

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">T47D cells arrested at G2M and Hyperploidy Formation Induced by a Curcumin’s Analogue PGV-1</dc:title>
	<dc:creator>Da’i, Muhammad</dc:creator>
	<dc:creator>Jenie, Umar Anggara</dc:creator>
	<dc:creator>AM, Supardjan</dc:creator>
	<dc:creator>Kawaichi, Masashi</dc:creator>
	<dc:creator>Meiyanto, Edy</dc:creator>
	<dc:description xml:lang="en-US">its chemical structure than curcumin. As a curcumin analogue, PGV-1 was considered to have anticanceractivities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cytotoxiceffects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progressionwas carried out using flowcytometry. Western blot analysis was used to analyze protein expression correspondingto cell cycle progression. The result showed that at the concentration of 2.5 μM PGV-1 inhibited cell cycleprogression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation inducedby PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 μM elevated the level of p21 CIP/KIPthrough p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated byPARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibitsthe growth of T47D cells targeted on microtubules.Keywords: PGV-1, G2/M arrest, apoptosis, p21</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:date>2007-12-09</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7776</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7776</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 12, No 2 (2007)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7776/6026</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>