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An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes

Indonesian Journal of Biotechnology · Vol. 15 No. 1 (2010)

DOI
10.22146/ijbiotech.7817
ISSN
2089-2241, 0853-8654
Terbit
2010-06-02
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

Actinomycetes have been the most prolific producer of various kinds of antifungal metabolites, and many of them are described as being produced by polyketide synthetases (pks). We present strain of Actinomycetes producing anticandida isolated from rhizosphere plant for amplification of Pks-I genes. The isolate was obtained from Wanagama I Forest UGM Yogyakarta. Gene of seven isolates, from total of 173 isolates, were amplified using degenerate primer to detect the presence of pks genes. One strain that is named Streptomyces sp. GMR-22 was partialy identified as anticandida producing actinomycete. The strain shown the strongest activity against Candida albicans. Based on bioautography assay, one spot active with Rf 0.57 was appeared as bright yellow by cerrium sulphate but it was and not visible on UV254 and 366 lights. Key words: pks genes, anticandida, Streptomyces sp GMR-22, rep-PCR, cajuput rhizosphere

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  • Universitas Gadjah Mada
  • Hasanuddin University
  • IPB University
  • Sriwijaya University
  • Riau University
  • Universitas Muhammadiyah Semarang
  • Jenderal Soedirman University
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Rekan penulisOrganisasiArtikel bersama
Michael Haryadi WibowoUniversitas Gadjah Mada4
Wayan Tunas ArtamaUniversitas Gadjah Mada4
Tri WibawaUniversitas Gadjah Mada4
Sri NuryatiIPB University3
Siti KabirunUniversitas Gadjah Mada3
Irfan Dwidya PrijambadaUniversitas Gadjah Mada3
N. NgadimanUniversitas Gadjah Mada3
Arum SetiawanSriwijaya University2
Mammed SagiUniversitas Gadjah Mada2
Istriyati IstriyatiUniversitas Gadjah Mada2
Nastiti WijayantiUniversitas Gadjah Mada2
Utut WidyastutiIPB University2
Endang SemiartiUniversitas Gadjah Mada2
Nur Bambang Priyo UtomoIPB University2
Marsetyawan H. N. E. SoesatyoUniversitas Gadjah Mada2
Triyanto TriyantoUniversitas Gadjah Mada2
Nursyirwani NursyirwaniRiau University2
A. E. T. H. WahyuniUniversitas Gadjah Mada2
Sebastian MarginoUniversitas Gadjah Mada2
Widodo WidodoIPB University2
Sri DarmawatiUniversitas Muhammadiyah Semarang2
Langkah SembiringUniversitas Gadjah Mada2
Donny WidiantoUniversitas Gadjah Mada2
Sri WedhastriUniversitas Gadjah Mada2
Tri UntariAfiliasi tidak diketahui2
Diani MentariJenderal Soedirman University1
Andi Aliah HidayaniHasanuddin University1
Dody Dh. TrijunoHasanuddin University1
Yushinta FujayaHasanuddin University1
Basse Siang ParawansaHasanuddin University1
Moh. Tauhid UmarHasanuddin University1
Irmawati IrmawatiHasanuddin University1
Aidah Ambo Ala HusainHasanuddin University1
Asmi Citra Malina A.R. TassakkaHasanuddin University1
Nadiarti NadiartiIndonesia Open University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes</dc:title>
	<dc:creator>Alimuddin, A.</dc:creator>
	<dc:creator>Asmara, Widya</dc:creator>
	<dc:creator>Widada, Jaka</dc:creator>
	<dc:creator>Mustofa, M.</dc:creator>
	<dc:creator>Nurjasmi, Reni</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">Actinomycetes have been the most prolific producer of various kinds of antifungal metabolites, and many of them are described as being produced by polyketide synthetases (pks). We present strain of Actinomycetes producing anticandida isolated from rhizosphere plant for amplification of Pks-I genes. The isolate was obtained from Wanagama I Forest UGM Yogyakarta. Gene of seven isolates, from total of 173 isolates, were amplified using degenerate primer to detect the presence of pks genes. One strain that is named Streptomyces sp. GMR-22 was partialy identified as anticandida producing actinomycete. The strain shown the strongest activity against Candida albicans. Based on bioautography assay, one spot active with Rf 0.57 was appeared as bright yellow by cerrium sulphate but it was and not visible on UV254 and 366 lights. Key words : pks genes, anticandida, Streptomyces sp GMR-22, rep-PCR, cajuput rhizosphere</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2010-06-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7817</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7817</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 15, No 1 (2010)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7817/6058</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
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