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Characterization of envelope-transmembrane Gene of Jembrana Disease Virus Tabanan 1995 Isolate

Indonesian Journal of Biotechnology · Vol. 15 No. 1 (2010)

DOI
10.22146/ijbiotech.7819
ISSN
2089-2241, 0853-8654
Terbit
2010-06-02
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral disease as in this case early diagnosis is a critical factor in containing disease outbreaks. Jembrana Disease Virus (JDV) is a lentivirus that causes an acute, severe disease syndrome in infected Bali cattle in Indonesia, resulting in heavy economic losses because of the high mortalities. The virus-host interaction and the modes of transmission are still unknown. The goal of the research was to designa probe candidate of Jembrana Disease Virus based on envelope-transmembrane (env-tm) gene to optimize Jembrana disease detection method. The DNA fragment derived from env-tm of JDV was used, cloned in pGEX-TM and expressed in E.coli DH 5α. Sequence analysis was conducted with BLAST programs from NCBI. Sequence analyses of the fragments of env-tm clone, indicated that it has a very closed genetic relation with 97,68% homology identity. Probe was designed based on the conserved region of env-tm using Geneious resulted in JT2 252 bp long. BLAST analyses showed that probes had high specifity to other strains of JDV in Indonesia.Key words: probe, env-tm, JDV, specifity, sensitivity.

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  • Universitas Trisakti
  • Bandung Institute of Technology
  • Tadulako University
  • Universitas Halu Oleo
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  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Firman AlamsyahAfiliasi tidak diketahui4
Muhammad AnsoriAfiliasi tidak diketahui3
M. MurwantokoAfiliasi tidak diketahui3
Rumiyati RumiyatiAfiliasi tidak diketahui3
Hanni GarminiaBandung Institute of Technology3
Parwadi MoenginUniversitas Trisakti2
Sita NurmasitahAfiliasi tidak diketahui2
Wahyuningsih WahyuningsihTadulako University2
Siti FathonahAfiliasi tidak diketahui2
Saptariana SaptarianaAfiliasi tidak diketahui2
Hera MaheshwariAfiliasi tidak diketahui2
LUTHFIRALDA SJAHFIRDIAfiliasi tidak diketahui2
DONDIN SAJUTHIAfiliasi tidak diketahui2
sarmin sarminUniversitas Halu Oleo2
Claude Mona AirinAfiliasi tidak diketahui2
Woro Anindito Sri TunjungAfiliasi tidak diketahui2
Alfi Rizqi AmaliaAfiliasi tidak diketahui2
Yekti Asih PurwestriAfiliasi tidak diketahui2
Vita MahardhikaAfiliasi tidak diketahui2
Sabarinsyah SabarinsyahAfiliasi tidak diketahui2
Joni FatUniversitas Trisakti1
Sally CahyatiUniversitas Trisakti1
Ririn SetyowatiAfiliasi tidak diketahui1
Nursanti Izzatullaili AdnaniAfiliasi tidak diketahui1
Rosidah RosidahAfiliasi tidak diketahui1
Octavianti ParamitaAfiliasi tidak diketahui1
Najla Khairunnisa SalsabilaAfiliasi tidak diketahui1
Niamatul Khoiriyah LaurenAfiliasi tidak diketahui1
Evi RohmiyatiAfiliasi tidak diketahui1
SaptarianaAfiliasi tidak diketahui1
Afifah Fitria DestianaAfiliasi tidak diketahui1
Farda Zulaiha AsyariAfiliasi tidak diketahui1
Afrilia Dwi WicaksonoAfiliasi tidak diketahui1
Atika Nur FauziyahAfiliasi tidak diketahui1
Diyah Ayu Sari Arum DasihAfiliasi tidak diketahui1
Erika YulianaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Characterization of envelope-transmembrane Gene of Jembrana Disease Virus Tabanan 1995 Isolate</dc:title>
	<dc:creator>Kusumawati, Asmarani</dc:creator>
	<dc:creator>Pratiwi, Rarastoeti</dc:creator>
	<dc:creator>Astuti, Pudji</dc:creator>
	<dc:creator>Hamid, Penny Humaidah</dc:creator>
	<dc:description xml:lang="en-US">The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral disease as in this case early diagnosis is a critical factor in containing disease outbreaks. Jembrana Disease Virus (JDV) is a lentivirus that causes an acute, severe disease syndrome in infected Bali cattle in Indonesia, resulting in heavy economic losses because of the high mortalities. The virus-host interaction and the modes of transmission are still unknown. The goal of the research was to designa probe candidate of Jembrana Disease Virus based on envelope-transmembrane (env-tm) gene to optimize Jembrana disease detection method. The DNA fragment derived from env-tm of JDV was used, cloned in pGEX-TM and expressed in E.coli DH 5α. Sequence analysis was conducted with BLAST programs from NCBI. Sequence analyses of the fragments of env-tm clone, indicated that it has a very closed genetic relation with 97,68% homology identity. Probe was designed based on the conserved region of env-tm using Geneious resulted in JT2 252 bp long. BLAST analyses showed that probes had high specifity to other strains of JDV in Indonesia.Key words : probe, env-tm, JDV, specifity, sensitivity.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:date>2010-06-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7819</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7819</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 15, No 1 (2010)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7819/6060</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>