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Comparative Analysis of Rice Transformation Using Agrobacterium tumefaciens and Rhyzobium leguminosarum

Indonesian Journal of Biotechnology · Vol. 15 No. 1 (2010)

DOI
10.22146/ijbiotech.7821
ISSN
2089-2241, 0853-8654
Terbit
2010-06-02
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

This study was aimed to study the effectiveness of Rhizobium transformation system compared to the most widely used Agrobacterium mediated transformation system on three rice cultivars, Ciherang (Indica), Nipponbare (Japonica), and Rojolele (Javanica). Six day old calli induced from immature embryos were inoculated with Rhizobium leguminosarum bv trifolii ANU845 and Agrobacterium tumefaciens LBA288 that harbored with vector pCAMBIA 5106. This plasmid contained a minimum set of transfer machinery genes and had a gusplus and an hptII gene driven by 35S CaMV promoter in the T-DNA. The results showed that the transformation frequencies (number of PCR positive plants per number of calli inoculated) ranging from 0 to 12.05 % depend on the genotype and transfer agent used. The highest transformation frequency (12.05%) was obtained in Ciherang transformed with R. leguminosarum. Most of the transgenic rice obtainedby Rhizobium transformation were normal in morphology and fertile similar to those obtained by Agrobacterium transformation. Integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis in T0 and T1 generations.Key words: Rhizobium leguminosarum, immature embryos, Agrobacterium tumefaciens

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  • IPB University
  • University of Darussalam Gontor
  • Nara Institute of Science and Technology
  • National Research and Innovation Agency
  • Universitas Nusa Bangsa
  • Universitas Islam Negeri Maulana Malik Ibrahim
  • Southeast Asian Regional Centre for Tropical Biology
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Rekan penulisOrganisasiArtikel bersama
Utut WidyastutiIPB University3
Edi SantosaIPB University2
Lutfy Ditya CahyantiUniversity of Darussalam Gontor2
Heni PurnamawatiIPB University2
Yadi SetiadiIPB University2
Aris TjahjoleksonoIPB University2
Niken TrisnaningrumUniversity of Darussalam Gontor1
Lulut Dwi SulistyaningsihIPB University1
Hamim HamimUniversitas Islam Negeri Maulana Malik Ibrahim1
Luluk SetyaningsihUniversitas Nusa Bangsa1
Sri Wilarso BudiIPB University1
Miftahudin MiftahudinIPB University1
Erina SulistianiSoutheast Asian Regional Centre for Tropical Biology1
Ence Darmo Jaya SupenaIPB University1
Sulastri SulastriLampung University1
Arkan SetiajiIPB University1
SuharsonoNational Research and Innovation Agency1
Radite TistamaIPB University1
Akiho YokotaNara Institute of Science and Technology1
Kinya AkashiNara Institute of Science and Technology1
SuwarnoDiponegoro University1
Hajrial AswidinnoorIPB University1
TRIKOESOEMANINGTYASIPB University1
Angelita Puji LestariIPB University1
Fadliah SalimIPB University1
Mohamad YaniIPB University1
Sri HartatiAfiliasi tidak diketahui1
Enny SudarmonowatiNational Research and Innovation Agency1
Kurnia SofyanAfiliasi tidak diketahui1
Desriani DesrianiIndonesian Institute of Sciences1
Fuad Al-AhwaniIPB University1
Pasmawati PasmawatiIPB University1
Miftahudin MiftahudinIPB University1
Musawira MusawiraIPB University1
Agus Nur HidayahAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Comparative Analysis of Rice Transformation Using Agrobacterium tumefaciens and Rhyzobium leguminosarum</dc:title>
	<dc:creator>Rahmawati, Syamsidah</dc:creator>
	<dc:creator>Jefferson, Osmat Azzam</dc:creator>
	<dc:creator>Sopandie, Didy</dc:creator>
	<dc:creator>Suharsono, S.</dc:creator>
	<dc:creator>Slamet-Loedin, Inez Hortense</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">This study was aimed to study the effectiveness of Rhizobium transformation system compared to the most widely used Agrobacterium mediated transformation system on three rice cultivars, Ciherang (Indica), Nipponbare (Japonica), and Rojolele (Javanica). Six day old calli induced from immature embryos were inoculated with Rhizobium leguminosarum bv trifolii ANU845 and Agrobacterium tumefaciens LBA288 that harbored with vector pCAMBIA 5106. This plasmid contained a minimum set of transfer machinery genes and had a gusplus and an hptII gene driven by 35S CaMV promoter in the T-DNA. The results showed that the transformation frequencies (number of PCR positive plants per number of calli inoculated) ranging from 0 to 12.05 % depend on the genotype and transfer agent used. The highest transformation frequency (12.05%) was obtained in Ciherang transformed with R. leguminosarum. Most of the transgenic rice obtainedby Rhizobium transformation were normal in morphology and fertile similar to those obtained by Agrobacterium transformation. Integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis in T0 and T1 generations.Key words : Rhizobium leguminosarum, immature embryos, Agrobacterium tumefaciens</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2010-06-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7821</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7821</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 15, No 1 (2010)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7821/6062</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
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